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Effects of Schizolobium parahyba Extract on Experimental Bothrops Venom-Induced Acute Kidney Injury
BACKGROUND: Venom-induced acute kidney injury (AKI) is a frequent complication of Bothrops snakebite with relevant morbidity and mortality. The aim of this study was to assess the effects of Schizolobium parahyba (SP) extract, a natural medicine with presumed anti-Bothrops venom effects, in an exper...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3925091/ https://www.ncbi.nlm.nih.gov/pubmed/24551041 http://dx.doi.org/10.1371/journal.pone.0086828 |
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author | Martines, Monique Silva Mendes, Mirian M. Shimizu, Maria H. M. Melo Rodrigues, Veridiana de Castro, Isac Filho, Sebastião R. Ferreira Malheiros, Denise M. A. C. Yu, Luis Burdmann, Emmanuel A. |
author_facet | Martines, Monique Silva Mendes, Mirian M. Shimizu, Maria H. M. Melo Rodrigues, Veridiana de Castro, Isac Filho, Sebastião R. Ferreira Malheiros, Denise M. A. C. Yu, Luis Burdmann, Emmanuel A. |
author_sort | Martines, Monique Silva |
collection | PubMed |
description | BACKGROUND: Venom-induced acute kidney injury (AKI) is a frequent complication of Bothrops snakebite with relevant morbidity and mortality. The aim of this study was to assess the effects of Schizolobium parahyba (SP) extract, a natural medicine with presumed anti-Bothrops venom effects, in an experimental model of Bothrops jararaca venom (BV)-induced AKI. METHODOLOGY: Groups of 8 to 10 rats received infusions of 0.9% saline (control, C), SP 2 mg/kg, BV 0.25 mg/kg and BV immediately followed by SP (treatment, T) in the doses already described. After the respective infusions, animals were assessed for their glomerular filtration rate (GFR, inulin clearance), renal blood flow (RBF, Doppler), blood pressure (BP, intra-arterial transducer), renal vascular resistance (RVR), urinary osmolality (UO, freezing point), urinary neutrophil gelatinase-associated lipocalin (NGAL, enzyme-linked immunosorbent assay [ELISA]), lactate dehydrogenase (LDH, kinetic method), hematocrit (Hct, microhematocrit), fibrinogen (Fi, Klauss modified) and blinded renal histology (acute tubular necrosis score). PRINCIPAL FINDINGS: BV caused significant decreases in GFR, RBF, UO, HcT and Fi; significant increases in RVR, NGAL and LDH; and acute tubular necrosis. SP did not prevent these changes; instead, it caused a significant decrease in GFR when used alone. CONCLUSION: SP administered simultaneously with BV, in an approximate 10∶1 concentration, did not prevent BV-induced AKI, hemolysis and fibrinogen consumption. SP used alone caused a decrease in GFR. |
format | Online Article Text |
id | pubmed-3925091 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-39250912014-02-18 Effects of Schizolobium parahyba Extract on Experimental Bothrops Venom-Induced Acute Kidney Injury Martines, Monique Silva Mendes, Mirian M. Shimizu, Maria H. M. Melo Rodrigues, Veridiana de Castro, Isac Filho, Sebastião R. Ferreira Malheiros, Denise M. A. C. Yu, Luis Burdmann, Emmanuel A. PLoS One Research Article BACKGROUND: Venom-induced acute kidney injury (AKI) is a frequent complication of Bothrops snakebite with relevant morbidity and mortality. The aim of this study was to assess the effects of Schizolobium parahyba (SP) extract, a natural medicine with presumed anti-Bothrops venom effects, in an experimental model of Bothrops jararaca venom (BV)-induced AKI. METHODOLOGY: Groups of 8 to 10 rats received infusions of 0.9% saline (control, C), SP 2 mg/kg, BV 0.25 mg/kg and BV immediately followed by SP (treatment, T) in the doses already described. After the respective infusions, animals were assessed for their glomerular filtration rate (GFR, inulin clearance), renal blood flow (RBF, Doppler), blood pressure (BP, intra-arterial transducer), renal vascular resistance (RVR), urinary osmolality (UO, freezing point), urinary neutrophil gelatinase-associated lipocalin (NGAL, enzyme-linked immunosorbent assay [ELISA]), lactate dehydrogenase (LDH, kinetic method), hematocrit (Hct, microhematocrit), fibrinogen (Fi, Klauss modified) and blinded renal histology (acute tubular necrosis score). PRINCIPAL FINDINGS: BV caused significant decreases in GFR, RBF, UO, HcT and Fi; significant increases in RVR, NGAL and LDH; and acute tubular necrosis. SP did not prevent these changes; instead, it caused a significant decrease in GFR when used alone. CONCLUSION: SP administered simultaneously with BV, in an approximate 10∶1 concentration, did not prevent BV-induced AKI, hemolysis and fibrinogen consumption. SP used alone caused a decrease in GFR. Public Library of Science 2014-02-14 /pmc/articles/PMC3925091/ /pubmed/24551041 http://dx.doi.org/10.1371/journal.pone.0086828 Text en © 2014 Martines et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Martines, Monique Silva Mendes, Mirian M. Shimizu, Maria H. M. Melo Rodrigues, Veridiana de Castro, Isac Filho, Sebastião R. Ferreira Malheiros, Denise M. A. C. Yu, Luis Burdmann, Emmanuel A. Effects of Schizolobium parahyba Extract on Experimental Bothrops Venom-Induced Acute Kidney Injury |
title | Effects of Schizolobium parahyba Extract on Experimental Bothrops Venom-Induced Acute Kidney Injury |
title_full | Effects of Schizolobium parahyba Extract on Experimental Bothrops Venom-Induced Acute Kidney Injury |
title_fullStr | Effects of Schizolobium parahyba Extract on Experimental Bothrops Venom-Induced Acute Kidney Injury |
title_full_unstemmed | Effects of Schizolobium parahyba Extract on Experimental Bothrops Venom-Induced Acute Kidney Injury |
title_short | Effects of Schizolobium parahyba Extract on Experimental Bothrops Venom-Induced Acute Kidney Injury |
title_sort | effects of schizolobium parahyba extract on experimental bothrops venom-induced acute kidney injury |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3925091/ https://www.ncbi.nlm.nih.gov/pubmed/24551041 http://dx.doi.org/10.1371/journal.pone.0086828 |
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