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Role of nanostructured gold surfaces on monocyte activation and Staphylococcus epidermidis biofilm formation

The role of material surface properties in the direct interaction with bacteria and the indirect route via host defense cells is not fully understood. Recently, it was suggested that nanostructured implant surfaces possess antimicrobial properties. In the current study, the adhesion and biofilm form...

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Autores principales: Svensson, Sara, Forsberg, Magnus, Hulander, Mats, Vazirisani, Forugh, Palmquist, Anders, Lausmaa, Jukka, Thomsen, Peter, Trobos, Margarita
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3925225/
https://www.ncbi.nlm.nih.gov/pubmed/24550671
http://dx.doi.org/10.2147/IJN.S51465
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author Svensson, Sara
Forsberg, Magnus
Hulander, Mats
Vazirisani, Forugh
Palmquist, Anders
Lausmaa, Jukka
Thomsen, Peter
Trobos, Margarita
author_facet Svensson, Sara
Forsberg, Magnus
Hulander, Mats
Vazirisani, Forugh
Palmquist, Anders
Lausmaa, Jukka
Thomsen, Peter
Trobos, Margarita
author_sort Svensson, Sara
collection PubMed
description The role of material surface properties in the direct interaction with bacteria and the indirect route via host defense cells is not fully understood. Recently, it was suggested that nanostructured implant surfaces possess antimicrobial properties. In the current study, the adhesion and biofilm formation of Staphylococcus epidermidis and human monocyte adhesion and activation were studied separately and in coculture in different in vitro models using smooth gold and well-defined nanostructured gold surfaces. Two polystyrene surfaces were used as controls in the monocyte experiments. Fluorescent viability staining demonstrated a reduction in the viability of S. epidermidis close to the nanostructured gold surface, whereas the smooth gold correlated with more live biofilm. The results were supported by scanning electron microscopy observations, showing higher biofilm tower formations and more mature biofilms on smooth gold compared with nanostructured gold. Unstimulated monocytes on the different substrates demonstrated low activation, reduced gene expression of pro- and anti-inflammatory cytokines, and low cytokine secretion. In contrast, stimulation with opsonized zymosan or opsonized live S. epidermidis for 1 hour significantly increased the production of reactive oxygen species, the gene expression of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), IL-6, and IL-10, as well as the secretion of TNF-α, demonstrating the ability of the cells to elicit a response and actively phagocytose prey. In addition, cells cultured on the smooth gold and the nanostructured gold displayed a different adhesion pattern and a more rapid oxidative burst than those cultured on polystyrene upon stimulation. We conclude that S. epidermidis decreased its viability initially when adhering to nanostructured surfaces compared with smooth gold surfaces, especially in the bacterial cell layers closest to the surface. In contrast, material surface properties neither strongly promoted nor attenuated the activity of monocytes when exposed to zymosan particles or S. epidermidis.
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spelling pubmed-39252252014-02-18 Role of nanostructured gold surfaces on monocyte activation and Staphylococcus epidermidis biofilm formation Svensson, Sara Forsberg, Magnus Hulander, Mats Vazirisani, Forugh Palmquist, Anders Lausmaa, Jukka Thomsen, Peter Trobos, Margarita Int J Nanomedicine Original Research The role of material surface properties in the direct interaction with bacteria and the indirect route via host defense cells is not fully understood. Recently, it was suggested that nanostructured implant surfaces possess antimicrobial properties. In the current study, the adhesion and biofilm formation of Staphylococcus epidermidis and human monocyte adhesion and activation were studied separately and in coculture in different in vitro models using smooth gold and well-defined nanostructured gold surfaces. Two polystyrene surfaces were used as controls in the monocyte experiments. Fluorescent viability staining demonstrated a reduction in the viability of S. epidermidis close to the nanostructured gold surface, whereas the smooth gold correlated with more live biofilm. The results were supported by scanning electron microscopy observations, showing higher biofilm tower formations and more mature biofilms on smooth gold compared with nanostructured gold. Unstimulated monocytes on the different substrates demonstrated low activation, reduced gene expression of pro- and anti-inflammatory cytokines, and low cytokine secretion. In contrast, stimulation with opsonized zymosan or opsonized live S. epidermidis for 1 hour significantly increased the production of reactive oxygen species, the gene expression of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), IL-6, and IL-10, as well as the secretion of TNF-α, demonstrating the ability of the cells to elicit a response and actively phagocytose prey. In addition, cells cultured on the smooth gold and the nanostructured gold displayed a different adhesion pattern and a more rapid oxidative burst than those cultured on polystyrene upon stimulation. We conclude that S. epidermidis decreased its viability initially when adhering to nanostructured surfaces compared with smooth gold surfaces, especially in the bacterial cell layers closest to the surface. In contrast, material surface properties neither strongly promoted nor attenuated the activity of monocytes when exposed to zymosan particles or S. epidermidis. Dove Medical Press 2014-02-07 /pmc/articles/PMC3925225/ /pubmed/24550671 http://dx.doi.org/10.2147/IJN.S51465 Text en © 2014 Svensson et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Svensson, Sara
Forsberg, Magnus
Hulander, Mats
Vazirisani, Forugh
Palmquist, Anders
Lausmaa, Jukka
Thomsen, Peter
Trobos, Margarita
Role of nanostructured gold surfaces on monocyte activation and Staphylococcus epidermidis biofilm formation
title Role of nanostructured gold surfaces on monocyte activation and Staphylococcus epidermidis biofilm formation
title_full Role of nanostructured gold surfaces on monocyte activation and Staphylococcus epidermidis biofilm formation
title_fullStr Role of nanostructured gold surfaces on monocyte activation and Staphylococcus epidermidis biofilm formation
title_full_unstemmed Role of nanostructured gold surfaces on monocyte activation and Staphylococcus epidermidis biofilm formation
title_short Role of nanostructured gold surfaces on monocyte activation and Staphylococcus epidermidis biofilm formation
title_sort role of nanostructured gold surfaces on monocyte activation and staphylococcus epidermidis biofilm formation
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3925225/
https://www.ncbi.nlm.nih.gov/pubmed/24550671
http://dx.doi.org/10.2147/IJN.S51465
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