Cargando…

RIVETS: A Mechanical System for In Vivo and In Vitro Electrophysiology and Imaging

A number of recent studies have provided compelling demonstrations that both mice and rats can be trained to perform a variety of behavioral tasks while restrained by mechanical elements mounted to the skull. The independent development of this technique by a number of laboratories has led to divers...

Descripción completa

Detalles Bibliográficos
Autores principales: Osborne, Jason E., Dudman, Joshua T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3925229/
https://www.ncbi.nlm.nih.gov/pubmed/24551206
http://dx.doi.org/10.1371/journal.pone.0089007
_version_ 1782303834503118848
author Osborne, Jason E.
Dudman, Joshua T.
author_facet Osborne, Jason E.
Dudman, Joshua T.
author_sort Osborne, Jason E.
collection PubMed
description A number of recent studies have provided compelling demonstrations that both mice and rats can be trained to perform a variety of behavioral tasks while restrained by mechanical elements mounted to the skull. The independent development of this technique by a number of laboratories has led to diverse solutions. We found that these solutions often used expensive materials and impeded future development and modification in the absence of engineering support. In order to address these issues, here we report on the development of a flexible single hardware design for electrophysiology and imaging both in brain tissue in vitro. Our hardware facilitates the rapid conversion of a single preparation between physiology and imaging system and the conversion of a given system between preparations. In addition, our use of rapid prototyping machines (“3D printers”) allows for the deployment of new designs within a day. Here, we present specifications for design and manufacturing as well as some data from our lab demonstrating the suitability of the design for physiology in behaving animals and imaging in vitro and in vivo.
format Online
Article
Text
id pubmed-3925229
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-39252292014-02-18 RIVETS: A Mechanical System for In Vivo and In Vitro Electrophysiology and Imaging Osborne, Jason E. Dudman, Joshua T. PLoS One Research Article A number of recent studies have provided compelling demonstrations that both mice and rats can be trained to perform a variety of behavioral tasks while restrained by mechanical elements mounted to the skull. The independent development of this technique by a number of laboratories has led to diverse solutions. We found that these solutions often used expensive materials and impeded future development and modification in the absence of engineering support. In order to address these issues, here we report on the development of a flexible single hardware design for electrophysiology and imaging both in brain tissue in vitro. Our hardware facilitates the rapid conversion of a single preparation between physiology and imaging system and the conversion of a given system between preparations. In addition, our use of rapid prototyping machines (“3D printers”) allows for the deployment of new designs within a day. Here, we present specifications for design and manufacturing as well as some data from our lab demonstrating the suitability of the design for physiology in behaving animals and imaging in vitro and in vivo. Public Library of Science 2014-02-14 /pmc/articles/PMC3925229/ /pubmed/24551206 http://dx.doi.org/10.1371/journal.pone.0089007 Text en © 2014 Osborne, Dudman http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Osborne, Jason E.
Dudman, Joshua T.
RIVETS: A Mechanical System for In Vivo and In Vitro Electrophysiology and Imaging
title RIVETS: A Mechanical System for In Vivo and In Vitro Electrophysiology and Imaging
title_full RIVETS: A Mechanical System for In Vivo and In Vitro Electrophysiology and Imaging
title_fullStr RIVETS: A Mechanical System for In Vivo and In Vitro Electrophysiology and Imaging
title_full_unstemmed RIVETS: A Mechanical System for In Vivo and In Vitro Electrophysiology and Imaging
title_short RIVETS: A Mechanical System for In Vivo and In Vitro Electrophysiology and Imaging
title_sort rivets: a mechanical system for in vivo and in vitro electrophysiology and imaging
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3925229/
https://www.ncbi.nlm.nih.gov/pubmed/24551206
http://dx.doi.org/10.1371/journal.pone.0089007
work_keys_str_mv AT osbornejasone rivetsamechanicalsystemforinvivoandinvitroelectrophysiologyandimaging
AT dudmanjoshuat rivetsamechanicalsystemforinvivoandinvitroelectrophysiologyandimaging