Cargando…

Dysbiosis of Salivary Microbiota in Inflammatory Bowel Disease and Its Association With Oral Immunological Biomarkers

Analysis of microbiota in various biological and environmental samples under a variety of conditions has recently become more practical due to remarkable advances in next-generation sequencing. Changes leading to specific biological states including some of the more complex diseases can now be chara...

Descripción completa

Detalles Bibliográficos
Autores principales: Said, Heba S., Suda, Wataru, Nakagome, Shigeki, Chinen, Hiroshi, Oshima, Kenshiro, Kim, Sangwan, Kimura, Ryosuke, Iraha, Atsushi, Ishida, Hajime, Fujita, Jiro, Mano, Shuhei, Morita, Hidetoshi, Dohi, Taeko, Oota, Hiroki, Hattori, Masahira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3925391/
https://www.ncbi.nlm.nih.gov/pubmed/24013298
http://dx.doi.org/10.1093/dnares/dst037
_version_ 1782303854576009216
author Said, Heba S.
Suda, Wataru
Nakagome, Shigeki
Chinen, Hiroshi
Oshima, Kenshiro
Kim, Sangwan
Kimura, Ryosuke
Iraha, Atsushi
Ishida, Hajime
Fujita, Jiro
Mano, Shuhei
Morita, Hidetoshi
Dohi, Taeko
Oota, Hiroki
Hattori, Masahira
author_facet Said, Heba S.
Suda, Wataru
Nakagome, Shigeki
Chinen, Hiroshi
Oshima, Kenshiro
Kim, Sangwan
Kimura, Ryosuke
Iraha, Atsushi
Ishida, Hajime
Fujita, Jiro
Mano, Shuhei
Morita, Hidetoshi
Dohi, Taeko
Oota, Hiroki
Hattori, Masahira
author_sort Said, Heba S.
collection PubMed
description Analysis of microbiota in various biological and environmental samples under a variety of conditions has recently become more practical due to remarkable advances in next-generation sequencing. Changes leading to specific biological states including some of the more complex diseases can now be characterized with relative ease. It is known that gut microbiota is involved in the pathogenesis of inflammatory bowel disease (IBD), mainly Crohn's disease and ulcerative colitis, exhibiting symptoms in the gastrointestinal tract. Recent studies also showed increased frequency of oral manifestations among IBD patients, indicating aberrations in the oral microbiota. Based on these observations, we analyzed the composition of salivary microbiota of 35 IBD patients by 454 pyrosequencing of the bacterial 16S rRNA gene and compared it with that of 24 healthy controls (HCs). The results showed that Bacteroidetes was significantly increased with a concurrent decrease in Proteobacteria in the salivary microbiota of IBD patients. The dominant genera, Streptococcus, Prevotella, Neisseria, Haemophilus, Veillonella, and Gemella, were found to largely contribute to dysbiosis (dysbacteriosis) observed in the salivary microbiota of IBD patients. Analysis of immunological biomarkers in the saliva of IBD patients showed elevated levels of many inflammatory cytokines and immunoglobulin A, and a lower lysozyme level. A strong correlation was shown between lysozyme and IL-1β levels and the relative abundance of Streptococcus, Prevotella, Haemophilus and Veillonella. Our data demonstrate that dysbiosis of salivary microbiota is associated with inflammatory responses in IBD patients, suggesting that it is possibly linked to dysbiosis of their gut microbiota.
format Online
Article
Text
id pubmed-3925391
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Oxford University Press
record_format MEDLINE/PubMed
spelling pubmed-39253912014-02-21 Dysbiosis of Salivary Microbiota in Inflammatory Bowel Disease and Its Association With Oral Immunological Biomarkers Said, Heba S. Suda, Wataru Nakagome, Shigeki Chinen, Hiroshi Oshima, Kenshiro Kim, Sangwan Kimura, Ryosuke Iraha, Atsushi Ishida, Hajime Fujita, Jiro Mano, Shuhei Morita, Hidetoshi Dohi, Taeko Oota, Hiroki Hattori, Masahira DNA Res Full Papers Analysis of microbiota in various biological and environmental samples under a variety of conditions has recently become more practical due to remarkable advances in next-generation sequencing. Changes leading to specific biological states including some of the more complex diseases can now be characterized with relative ease. It is known that gut microbiota is involved in the pathogenesis of inflammatory bowel disease (IBD), mainly Crohn's disease and ulcerative colitis, exhibiting symptoms in the gastrointestinal tract. Recent studies also showed increased frequency of oral manifestations among IBD patients, indicating aberrations in the oral microbiota. Based on these observations, we analyzed the composition of salivary microbiota of 35 IBD patients by 454 pyrosequencing of the bacterial 16S rRNA gene and compared it with that of 24 healthy controls (HCs). The results showed that Bacteroidetes was significantly increased with a concurrent decrease in Proteobacteria in the salivary microbiota of IBD patients. The dominant genera, Streptococcus, Prevotella, Neisseria, Haemophilus, Veillonella, and Gemella, were found to largely contribute to dysbiosis (dysbacteriosis) observed in the salivary microbiota of IBD patients. Analysis of immunological biomarkers in the saliva of IBD patients showed elevated levels of many inflammatory cytokines and immunoglobulin A, and a lower lysozyme level. A strong correlation was shown between lysozyme and IL-1β levels and the relative abundance of Streptococcus, Prevotella, Haemophilus and Veillonella. Our data demonstrate that dysbiosis of salivary microbiota is associated with inflammatory responses in IBD patients, suggesting that it is possibly linked to dysbiosis of their gut microbiota. Oxford University Press 2014-02 2013-09-07 /pmc/articles/PMC3925391/ /pubmed/24013298 http://dx.doi.org/10.1093/dnares/dst037 Text en © The Author 2013. Published by Oxford University Press on behalf of Kazusa DNA Research Institute. http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Full Papers
Said, Heba S.
Suda, Wataru
Nakagome, Shigeki
Chinen, Hiroshi
Oshima, Kenshiro
Kim, Sangwan
Kimura, Ryosuke
Iraha, Atsushi
Ishida, Hajime
Fujita, Jiro
Mano, Shuhei
Morita, Hidetoshi
Dohi, Taeko
Oota, Hiroki
Hattori, Masahira
Dysbiosis of Salivary Microbiota in Inflammatory Bowel Disease and Its Association With Oral Immunological Biomarkers
title Dysbiosis of Salivary Microbiota in Inflammatory Bowel Disease and Its Association With Oral Immunological Biomarkers
title_full Dysbiosis of Salivary Microbiota in Inflammatory Bowel Disease and Its Association With Oral Immunological Biomarkers
title_fullStr Dysbiosis of Salivary Microbiota in Inflammatory Bowel Disease and Its Association With Oral Immunological Biomarkers
title_full_unstemmed Dysbiosis of Salivary Microbiota in Inflammatory Bowel Disease and Its Association With Oral Immunological Biomarkers
title_short Dysbiosis of Salivary Microbiota in Inflammatory Bowel Disease and Its Association With Oral Immunological Biomarkers
title_sort dysbiosis of salivary microbiota in inflammatory bowel disease and its association with oral immunological biomarkers
topic Full Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3925391/
https://www.ncbi.nlm.nih.gov/pubmed/24013298
http://dx.doi.org/10.1093/dnares/dst037
work_keys_str_mv AT saidhebas dysbiosisofsalivarymicrobiotaininflammatoryboweldiseaseanditsassociationwithoralimmunologicalbiomarkers
AT sudawataru dysbiosisofsalivarymicrobiotaininflammatoryboweldiseaseanditsassociationwithoralimmunologicalbiomarkers
AT nakagomeshigeki dysbiosisofsalivarymicrobiotaininflammatoryboweldiseaseanditsassociationwithoralimmunologicalbiomarkers
AT chinenhiroshi dysbiosisofsalivarymicrobiotaininflammatoryboweldiseaseanditsassociationwithoralimmunologicalbiomarkers
AT oshimakenshiro dysbiosisofsalivarymicrobiotaininflammatoryboweldiseaseanditsassociationwithoralimmunologicalbiomarkers
AT kimsangwan dysbiosisofsalivarymicrobiotaininflammatoryboweldiseaseanditsassociationwithoralimmunologicalbiomarkers
AT kimuraryosuke dysbiosisofsalivarymicrobiotaininflammatoryboweldiseaseanditsassociationwithoralimmunologicalbiomarkers
AT irahaatsushi dysbiosisofsalivarymicrobiotaininflammatoryboweldiseaseanditsassociationwithoralimmunologicalbiomarkers
AT ishidahajime dysbiosisofsalivarymicrobiotaininflammatoryboweldiseaseanditsassociationwithoralimmunologicalbiomarkers
AT fujitajiro dysbiosisofsalivarymicrobiotaininflammatoryboweldiseaseanditsassociationwithoralimmunologicalbiomarkers
AT manoshuhei dysbiosisofsalivarymicrobiotaininflammatoryboweldiseaseanditsassociationwithoralimmunologicalbiomarkers
AT moritahidetoshi dysbiosisofsalivarymicrobiotaininflammatoryboweldiseaseanditsassociationwithoralimmunologicalbiomarkers
AT dohitaeko dysbiosisofsalivarymicrobiotaininflammatoryboweldiseaseanditsassociationwithoralimmunologicalbiomarkers
AT ootahiroki dysbiosisofsalivarymicrobiotaininflammatoryboweldiseaseanditsassociationwithoralimmunologicalbiomarkers
AT hattorimasahira dysbiosisofsalivarymicrobiotaininflammatoryboweldiseaseanditsassociationwithoralimmunologicalbiomarkers