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Assembly and analysis of a male sterile rubber tree mitochondrial genome reveals DNA rearrangement events and a novel transcript

BACKGROUND: The rubber tree, Hevea brasiliensis, is an important plant species that is commercially grown to produce latex rubber in many countries. The rubber tree variety BPM 24 exhibits cytoplasmic male sterility, inherited from the variety GT 1. RESULTS: We constructed the rubber tree mitochondr...

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Autores principales: Shearman, Jeremy R, Sangsrakru, Duangjai, Ruang-areerate, Panthita, Sonthirod, Chutima, Uthaipaisanwong, Pichahpuk, Yoocha, Thippawan, Poopear, Supannee, Theerawattanasuk, Kanikar, Tragoonrung, Somvong, Tangphatsornruang, Sithichoke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3925788/
https://www.ncbi.nlm.nih.gov/pubmed/24512148
http://dx.doi.org/10.1186/1471-2229-14-45
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author Shearman, Jeremy R
Sangsrakru, Duangjai
Ruang-areerate, Panthita
Sonthirod, Chutima
Uthaipaisanwong, Pichahpuk
Yoocha, Thippawan
Poopear, Supannee
Theerawattanasuk, Kanikar
Tragoonrung, Somvong
Tangphatsornruang, Sithichoke
author_facet Shearman, Jeremy R
Sangsrakru, Duangjai
Ruang-areerate, Panthita
Sonthirod, Chutima
Uthaipaisanwong, Pichahpuk
Yoocha, Thippawan
Poopear, Supannee
Theerawattanasuk, Kanikar
Tragoonrung, Somvong
Tangphatsornruang, Sithichoke
author_sort Shearman, Jeremy R
collection PubMed
description BACKGROUND: The rubber tree, Hevea brasiliensis, is an important plant species that is commercially grown to produce latex rubber in many countries. The rubber tree variety BPM 24 exhibits cytoplasmic male sterility, inherited from the variety GT 1. RESULTS: We constructed the rubber tree mitochondrial genome of a cytoplasmic male sterile variety, BPM 24, using 454 sequencing, including 8 kb paired-end libraries, plus Illumina paired-end sequencing. We annotated this mitochondrial genome with the aid of Illumina RNA-seq data and performed comparative analysis. We then compared the sequence of BPM 24 to the contigs of the published rubber tree, variety RRIM 600, and identified a rearrangement that is unique to BPM 24 resulting in a novel transcript containing a portion of atp9. CONCLUSIONS: The novel transcript is consistent with changes that cause cytoplasmic male sterility through a slight reduction to ATP production efficiency. The exhaustive nature of the search rules out alternative causes and supports previous findings of novel transcripts causing cytoplasmic male sterility.
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spelling pubmed-39257882014-02-18 Assembly and analysis of a male sterile rubber tree mitochondrial genome reveals DNA rearrangement events and a novel transcript Shearman, Jeremy R Sangsrakru, Duangjai Ruang-areerate, Panthita Sonthirod, Chutima Uthaipaisanwong, Pichahpuk Yoocha, Thippawan Poopear, Supannee Theerawattanasuk, Kanikar Tragoonrung, Somvong Tangphatsornruang, Sithichoke BMC Plant Biol Research Article BACKGROUND: The rubber tree, Hevea brasiliensis, is an important plant species that is commercially grown to produce latex rubber in many countries. The rubber tree variety BPM 24 exhibits cytoplasmic male sterility, inherited from the variety GT 1. RESULTS: We constructed the rubber tree mitochondrial genome of a cytoplasmic male sterile variety, BPM 24, using 454 sequencing, including 8 kb paired-end libraries, plus Illumina paired-end sequencing. We annotated this mitochondrial genome with the aid of Illumina RNA-seq data and performed comparative analysis. We then compared the sequence of BPM 24 to the contigs of the published rubber tree, variety RRIM 600, and identified a rearrangement that is unique to BPM 24 resulting in a novel transcript containing a portion of atp9. CONCLUSIONS: The novel transcript is consistent with changes that cause cytoplasmic male sterility through a slight reduction to ATP production efficiency. The exhaustive nature of the search rules out alternative causes and supports previous findings of novel transcripts causing cytoplasmic male sterility. BioMed Central 2014-02-10 /pmc/articles/PMC3925788/ /pubmed/24512148 http://dx.doi.org/10.1186/1471-2229-14-45 Text en Copyright © 2014 Shearman et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Shearman, Jeremy R
Sangsrakru, Duangjai
Ruang-areerate, Panthita
Sonthirod, Chutima
Uthaipaisanwong, Pichahpuk
Yoocha, Thippawan
Poopear, Supannee
Theerawattanasuk, Kanikar
Tragoonrung, Somvong
Tangphatsornruang, Sithichoke
Assembly and analysis of a male sterile rubber tree mitochondrial genome reveals DNA rearrangement events and a novel transcript
title Assembly and analysis of a male sterile rubber tree mitochondrial genome reveals DNA rearrangement events and a novel transcript
title_full Assembly and analysis of a male sterile rubber tree mitochondrial genome reveals DNA rearrangement events and a novel transcript
title_fullStr Assembly and analysis of a male sterile rubber tree mitochondrial genome reveals DNA rearrangement events and a novel transcript
title_full_unstemmed Assembly and analysis of a male sterile rubber tree mitochondrial genome reveals DNA rearrangement events and a novel transcript
title_short Assembly and analysis of a male sterile rubber tree mitochondrial genome reveals DNA rearrangement events and a novel transcript
title_sort assembly and analysis of a male sterile rubber tree mitochondrial genome reveals dna rearrangement events and a novel transcript
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3925788/
https://www.ncbi.nlm.nih.gov/pubmed/24512148
http://dx.doi.org/10.1186/1471-2229-14-45
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