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Assembly and analysis of a male sterile rubber tree mitochondrial genome reveals DNA rearrangement events and a novel transcript
BACKGROUND: The rubber tree, Hevea brasiliensis, is an important plant species that is commercially grown to produce latex rubber in many countries. The rubber tree variety BPM 24 exhibits cytoplasmic male sterility, inherited from the variety GT 1. RESULTS: We constructed the rubber tree mitochondr...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3925788/ https://www.ncbi.nlm.nih.gov/pubmed/24512148 http://dx.doi.org/10.1186/1471-2229-14-45 |
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author | Shearman, Jeremy R Sangsrakru, Duangjai Ruang-areerate, Panthita Sonthirod, Chutima Uthaipaisanwong, Pichahpuk Yoocha, Thippawan Poopear, Supannee Theerawattanasuk, Kanikar Tragoonrung, Somvong Tangphatsornruang, Sithichoke |
author_facet | Shearman, Jeremy R Sangsrakru, Duangjai Ruang-areerate, Panthita Sonthirod, Chutima Uthaipaisanwong, Pichahpuk Yoocha, Thippawan Poopear, Supannee Theerawattanasuk, Kanikar Tragoonrung, Somvong Tangphatsornruang, Sithichoke |
author_sort | Shearman, Jeremy R |
collection | PubMed |
description | BACKGROUND: The rubber tree, Hevea brasiliensis, is an important plant species that is commercially grown to produce latex rubber in many countries. The rubber tree variety BPM 24 exhibits cytoplasmic male sterility, inherited from the variety GT 1. RESULTS: We constructed the rubber tree mitochondrial genome of a cytoplasmic male sterile variety, BPM 24, using 454 sequencing, including 8 kb paired-end libraries, plus Illumina paired-end sequencing. We annotated this mitochondrial genome with the aid of Illumina RNA-seq data and performed comparative analysis. We then compared the sequence of BPM 24 to the contigs of the published rubber tree, variety RRIM 600, and identified a rearrangement that is unique to BPM 24 resulting in a novel transcript containing a portion of atp9. CONCLUSIONS: The novel transcript is consistent with changes that cause cytoplasmic male sterility through a slight reduction to ATP production efficiency. The exhaustive nature of the search rules out alternative causes and supports previous findings of novel transcripts causing cytoplasmic male sterility. |
format | Online Article Text |
id | pubmed-3925788 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-39257882014-02-18 Assembly and analysis of a male sterile rubber tree mitochondrial genome reveals DNA rearrangement events and a novel transcript Shearman, Jeremy R Sangsrakru, Duangjai Ruang-areerate, Panthita Sonthirod, Chutima Uthaipaisanwong, Pichahpuk Yoocha, Thippawan Poopear, Supannee Theerawattanasuk, Kanikar Tragoonrung, Somvong Tangphatsornruang, Sithichoke BMC Plant Biol Research Article BACKGROUND: The rubber tree, Hevea brasiliensis, is an important plant species that is commercially grown to produce latex rubber in many countries. The rubber tree variety BPM 24 exhibits cytoplasmic male sterility, inherited from the variety GT 1. RESULTS: We constructed the rubber tree mitochondrial genome of a cytoplasmic male sterile variety, BPM 24, using 454 sequencing, including 8 kb paired-end libraries, plus Illumina paired-end sequencing. We annotated this mitochondrial genome with the aid of Illumina RNA-seq data and performed comparative analysis. We then compared the sequence of BPM 24 to the contigs of the published rubber tree, variety RRIM 600, and identified a rearrangement that is unique to BPM 24 resulting in a novel transcript containing a portion of atp9. CONCLUSIONS: The novel transcript is consistent with changes that cause cytoplasmic male sterility through a slight reduction to ATP production efficiency. The exhaustive nature of the search rules out alternative causes and supports previous findings of novel transcripts causing cytoplasmic male sterility. BioMed Central 2014-02-10 /pmc/articles/PMC3925788/ /pubmed/24512148 http://dx.doi.org/10.1186/1471-2229-14-45 Text en Copyright © 2014 Shearman et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Shearman, Jeremy R Sangsrakru, Duangjai Ruang-areerate, Panthita Sonthirod, Chutima Uthaipaisanwong, Pichahpuk Yoocha, Thippawan Poopear, Supannee Theerawattanasuk, Kanikar Tragoonrung, Somvong Tangphatsornruang, Sithichoke Assembly and analysis of a male sterile rubber tree mitochondrial genome reveals DNA rearrangement events and a novel transcript |
title | Assembly and analysis of a male sterile rubber tree mitochondrial genome reveals DNA rearrangement events and a novel transcript |
title_full | Assembly and analysis of a male sterile rubber tree mitochondrial genome reveals DNA rearrangement events and a novel transcript |
title_fullStr | Assembly and analysis of a male sterile rubber tree mitochondrial genome reveals DNA rearrangement events and a novel transcript |
title_full_unstemmed | Assembly and analysis of a male sterile rubber tree mitochondrial genome reveals DNA rearrangement events and a novel transcript |
title_short | Assembly and analysis of a male sterile rubber tree mitochondrial genome reveals DNA rearrangement events and a novel transcript |
title_sort | assembly and analysis of a male sterile rubber tree mitochondrial genome reveals dna rearrangement events and a novel transcript |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3925788/ https://www.ncbi.nlm.nih.gov/pubmed/24512148 http://dx.doi.org/10.1186/1471-2229-14-45 |
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