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Determination of Platycodin D and Platycodin D3 in Rat Plasma Using Liquid Chromatography-Tandem Mass Spectrometry

Platycodon grandiflorum has long been used as a traditional oriental medicine for respiratory disorder. Platycodin D (PD) is known as the main component isolated from the root of PG. A simple and rapid liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has been developed and validated...

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Autores principales: Kim, Tae-Hyun, Lee, Byung Eui, Kim, Eun Joo, Choi, Yong Seok, Lee, Keun-Sung, Kim, Hak Rim, Kim, Hyung-Gun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3926248/
https://www.ncbi.nlm.nih.gov/pubmed/24592150
http://dx.doi.org/10.1155/2014/231293
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author Kim, Tae-Hyun
Lee, Byung Eui
Kim, Eun Joo
Choi, Yong Seok
Lee, Keun-Sung
Kim, Hak Rim
Kim, Hyung-Gun
author_facet Kim, Tae-Hyun
Lee, Byung Eui
Kim, Eun Joo
Choi, Yong Seok
Lee, Keun-Sung
Kim, Hak Rim
Kim, Hyung-Gun
author_sort Kim, Tae-Hyun
collection PubMed
description Platycodon grandiflorum has long been used as a traditional oriental medicine for respiratory disorder. Platycodin D (PD) is known as the main component isolated from the root of PG. A simple and rapid liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has been developed and validated for the quantitation of PD in rat plasma. Quantitation was performed on a triple quadrupole mass spectrometer employing electrospray ionization and multiple reaction monitoring in positive ion mode. The total chromatographic run time was 4.0 min, and the calibration curves of PD were linear over the concentration range of 50–10,000 ng/mL in rat plasma. The coefficient of variation and relative error at five QC levels were 1.0 to 8.8% and 0.7 to 8.7%, respectively. After a single oral administration of 500 mg/kg and a single intravenous administration of 25 mg/kg of 3% PD extract (a PG extract including 3% of PD), platycodin D and platycodin D3 were detected and pharmacokinetic parameters were estimated. The oral bioavailability of platycodin D and platycodin D3 was 0.29% and 1.35% in rats at 500 mg/kg of 3% PD extract of PG, respectively. The present method can be applied to pharmacokinetic analysis of platycodins and platycosides of the PG.
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spelling pubmed-39262482014-03-03 Determination of Platycodin D and Platycodin D3 in Rat Plasma Using Liquid Chromatography-Tandem Mass Spectrometry Kim, Tae-Hyun Lee, Byung Eui Kim, Eun Joo Choi, Yong Seok Lee, Keun-Sung Kim, Hak Rim Kim, Hyung-Gun ScientificWorldJournal Research Article Platycodon grandiflorum has long been used as a traditional oriental medicine for respiratory disorder. Platycodin D (PD) is known as the main component isolated from the root of PG. A simple and rapid liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has been developed and validated for the quantitation of PD in rat plasma. Quantitation was performed on a triple quadrupole mass spectrometer employing electrospray ionization and multiple reaction monitoring in positive ion mode. The total chromatographic run time was 4.0 min, and the calibration curves of PD were linear over the concentration range of 50–10,000 ng/mL in rat plasma. The coefficient of variation and relative error at five QC levels were 1.0 to 8.8% and 0.7 to 8.7%, respectively. After a single oral administration of 500 mg/kg and a single intravenous administration of 25 mg/kg of 3% PD extract (a PG extract including 3% of PD), platycodin D and platycodin D3 were detected and pharmacokinetic parameters were estimated. The oral bioavailability of platycodin D and platycodin D3 was 0.29% and 1.35% in rats at 500 mg/kg of 3% PD extract of PG, respectively. The present method can be applied to pharmacokinetic analysis of platycodins and platycosides of the PG. Hindawi Publishing Corporation 2014-01-30 /pmc/articles/PMC3926248/ /pubmed/24592150 http://dx.doi.org/10.1155/2014/231293 Text en Copyright © 2014 Tae-Hyun Kim et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Kim, Tae-Hyun
Lee, Byung Eui
Kim, Eun Joo
Choi, Yong Seok
Lee, Keun-Sung
Kim, Hak Rim
Kim, Hyung-Gun
Determination of Platycodin D and Platycodin D3 in Rat Plasma Using Liquid Chromatography-Tandem Mass Spectrometry
title Determination of Platycodin D and Platycodin D3 in Rat Plasma Using Liquid Chromatography-Tandem Mass Spectrometry
title_full Determination of Platycodin D and Platycodin D3 in Rat Plasma Using Liquid Chromatography-Tandem Mass Spectrometry
title_fullStr Determination of Platycodin D and Platycodin D3 in Rat Plasma Using Liquid Chromatography-Tandem Mass Spectrometry
title_full_unstemmed Determination of Platycodin D and Platycodin D3 in Rat Plasma Using Liquid Chromatography-Tandem Mass Spectrometry
title_short Determination of Platycodin D and Platycodin D3 in Rat Plasma Using Liquid Chromatography-Tandem Mass Spectrometry
title_sort determination of platycodin d and platycodin d3 in rat plasma using liquid chromatography-tandem mass spectrometry
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3926248/
https://www.ncbi.nlm.nih.gov/pubmed/24592150
http://dx.doi.org/10.1155/2014/231293
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