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The role of stem cells in osteoarthritis: An experimental study in rabbits
INTRODUCTION: Osteoarthritis (OA) is a progressively debilitating disease that affects mostly cartilage, with associated changes in the bone. The increasing incidence of OA and an ageing population, coupled with insufficient therapeutic choices, has led to focus on the potential of stem cells as a n...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
British Editorial Society of Bone and Joint Surgery
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3926293/ https://www.ncbi.nlm.nih.gov/pubmed/24526748 http://dx.doi.org/10.1302/2046-3758.32.2000187 |
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author | Singh, A. Goel, S. C. Gupta, K. K. Kumar, M. Arun, G. R. Patil, H. Kumaraswamy, V. Jha, S. |
author_facet | Singh, A. Goel, S. C. Gupta, K. K. Kumar, M. Arun, G. R. Patil, H. Kumaraswamy, V. Jha, S. |
author_sort | Singh, A. |
collection | PubMed |
description | INTRODUCTION: Osteoarthritis (OA) is a progressively debilitating disease that affects mostly cartilage, with associated changes in the bone. The increasing incidence of OA and an ageing population, coupled with insufficient therapeutic choices, has led to focus on the potential of stem cells as a novel strategy for cartilage repair. METHODS: In this study, we used scaffold-free mesenchymal stem cells (MSCs) obtained from bone marrow in an experimental animal model of OA by direct intra-articular injection. MSCs were isolated from 2.8 kg white New Zealand rabbits. There were ten in the study group and ten in the control group. OA was induced by unilateral transection of the anterior cruciate ligament of the knee joint. At 12 weeks post-operatively, a single dose of 1 million cells suspended in 1 ml of medium was delivered to the injured knee by direct intra-articular injection. The control group received 1 ml of medium without cells. The knees were examined at 16 and 20 weeks following surgery. Repair was investigated radiologically, grossly and histologically using haematoxylin and eosin, Safranin-O and toluidine blue staining. RESULTS: Radiological assessment confirmed development of OA changes after 12 weeks. Rabbits receiving MSCs showed a lower degree of cartilage degeneration, osteophyte formation, and subchondral sclerosis than the control group at 20 weeks post-operatively. The quality of cartilage was significantly better in the cell-treated group compared with the control group after 20 weeks. CONCLUSIONS: Bone marrow-derived MSCs could be promising cell sources for the treatment of OA. Neither stem cell culture nor scaffolds are absolutely necessary for a favourable outcome. Cite this article: Bone Joint Res 2014;3:32–7. |
format | Online Article Text |
id | pubmed-3926293 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | British Editorial Society of Bone and Joint Surgery |
record_format | MEDLINE/PubMed |
spelling | pubmed-39262932014-02-26 The role of stem cells in osteoarthritis: An experimental study in rabbits Singh, A. Goel, S. C. Gupta, K. K. Kumar, M. Arun, G. R. Patil, H. Kumaraswamy, V. Jha, S. Bone Joint Res Research INTRODUCTION: Osteoarthritis (OA) is a progressively debilitating disease that affects mostly cartilage, with associated changes in the bone. The increasing incidence of OA and an ageing population, coupled with insufficient therapeutic choices, has led to focus on the potential of stem cells as a novel strategy for cartilage repair. METHODS: In this study, we used scaffold-free mesenchymal stem cells (MSCs) obtained from bone marrow in an experimental animal model of OA by direct intra-articular injection. MSCs were isolated from 2.8 kg white New Zealand rabbits. There were ten in the study group and ten in the control group. OA was induced by unilateral transection of the anterior cruciate ligament of the knee joint. At 12 weeks post-operatively, a single dose of 1 million cells suspended in 1 ml of medium was delivered to the injured knee by direct intra-articular injection. The control group received 1 ml of medium without cells. The knees were examined at 16 and 20 weeks following surgery. Repair was investigated radiologically, grossly and histologically using haematoxylin and eosin, Safranin-O and toluidine blue staining. RESULTS: Radiological assessment confirmed development of OA changes after 12 weeks. Rabbits receiving MSCs showed a lower degree of cartilage degeneration, osteophyte formation, and subchondral sclerosis than the control group at 20 weeks post-operatively. The quality of cartilage was significantly better in the cell-treated group compared with the control group after 20 weeks. CONCLUSIONS: Bone marrow-derived MSCs could be promising cell sources for the treatment of OA. Neither stem cell culture nor scaffolds are absolutely necessary for a favourable outcome. Cite this article: Bone Joint Res 2014;3:32–7. British Editorial Society of Bone and Joint Surgery 2014-02-01 /pmc/articles/PMC3926293/ /pubmed/24526748 http://dx.doi.org/10.1302/2046-3758.32.2000187 Text en ©2014 The British Editorial Society of Bone & Joint Surgery ©2014 The British Editorial Society of Bone & Joint Surgery. This is an open-access article distributed under the terms of the Creative Commons Attributions licence, which permits unrestricted use, distribution, and reproduction in any medium, but not for commercial gain, provided the original author and source are credited. |
spellingShingle | Research Singh, A. Goel, S. C. Gupta, K. K. Kumar, M. Arun, G. R. Patil, H. Kumaraswamy, V. Jha, S. The role of stem cells in osteoarthritis: An experimental study in rabbits |
title | The role of stem cells in osteoarthritis: An experimental study in rabbits |
title_full | The role of stem cells in osteoarthritis: An experimental study in rabbits |
title_fullStr | The role of stem cells in osteoarthritis: An experimental study in rabbits |
title_full_unstemmed | The role of stem cells in osteoarthritis: An experimental study in rabbits |
title_short | The role of stem cells in osteoarthritis: An experimental study in rabbits |
title_sort | role of stem cells in osteoarthritis: an experimental study in rabbits |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3926293/ https://www.ncbi.nlm.nih.gov/pubmed/24526748 http://dx.doi.org/10.1302/2046-3758.32.2000187 |
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