Bienzymatic Biosensor for Rapid Detection of Aspartame by Flow Injection Analysis

A rapid, simple and stable biosensor for aspartame detection was developed. Alcohol oxidase (AOX), carboxyl esterase (CaE) and bovine serum albumin (BSA) were immobilised with glutaraldehyde (GA) onto screen-printed electrodes modified with cobalt-phthalocyanine (CoPC). The biosensor response was fa...

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Detalles Bibliográficos
Autores principales: Radulescu, Maria-Cristina, Bucur, Bogdan, Bucur, Madalina-Petruta, Radu, Gabriel Lucian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3926600/
https://www.ncbi.nlm.nih.gov/pubmed/24412899
http://dx.doi.org/10.3390/s140101028
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author Radulescu, Maria-Cristina
Bucur, Bogdan
Bucur, Madalina-Petruta
Radu, Gabriel Lucian
author_facet Radulescu, Maria-Cristina
Bucur, Bogdan
Bucur, Madalina-Petruta
Radu, Gabriel Lucian
author_sort Radulescu, Maria-Cristina
collection PubMed
description A rapid, simple and stable biosensor for aspartame detection was developed. Alcohol oxidase (AOX), carboxyl esterase (CaE) and bovine serum albumin (BSA) were immobilised with glutaraldehyde (GA) onto screen-printed electrodes modified with cobalt-phthalocyanine (CoPC). The biosensor response was fast. The sample throughput using a flow injection analysis (FIA) system was 40 h(−1) with an RSD of 2.7%. The detection limits for both batch and FIA measurements were 0.1 μM for methanol and 0.2 μM for aspartame, respectively. The enzymatic biosensor was successfully applied for aspartame determination in different sample matrices/commercial products (liquid and solid samples) without any pre-treatment step prior to measurement.
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spelling pubmed-39266002014-02-18 Bienzymatic Biosensor for Rapid Detection of Aspartame by Flow Injection Analysis Radulescu, Maria-Cristina Bucur, Bogdan Bucur, Madalina-Petruta Radu, Gabriel Lucian Sensors (Basel) Article A rapid, simple and stable biosensor for aspartame detection was developed. Alcohol oxidase (AOX), carboxyl esterase (CaE) and bovine serum albumin (BSA) were immobilised with glutaraldehyde (GA) onto screen-printed electrodes modified with cobalt-phthalocyanine (CoPC). The biosensor response was fast. The sample throughput using a flow injection analysis (FIA) system was 40 h(−1) with an RSD of 2.7%. The detection limits for both batch and FIA measurements were 0.1 μM for methanol and 0.2 μM for aspartame, respectively. The enzymatic biosensor was successfully applied for aspartame determination in different sample matrices/commercial products (liquid and solid samples) without any pre-treatment step prior to measurement. Molecular Diversity Preservation International (MDPI) 2014-01-09 /pmc/articles/PMC3926600/ /pubmed/24412899 http://dx.doi.org/10.3390/s140101028 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Radulescu, Maria-Cristina
Bucur, Bogdan
Bucur, Madalina-Petruta
Radu, Gabriel Lucian
Bienzymatic Biosensor for Rapid Detection of Aspartame by Flow Injection Analysis
title Bienzymatic Biosensor for Rapid Detection of Aspartame by Flow Injection Analysis
title_full Bienzymatic Biosensor for Rapid Detection of Aspartame by Flow Injection Analysis
title_fullStr Bienzymatic Biosensor for Rapid Detection of Aspartame by Flow Injection Analysis
title_full_unstemmed Bienzymatic Biosensor for Rapid Detection of Aspartame by Flow Injection Analysis
title_short Bienzymatic Biosensor for Rapid Detection of Aspartame by Flow Injection Analysis
title_sort bienzymatic biosensor for rapid detection of aspartame by flow injection analysis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3926600/
https://www.ncbi.nlm.nih.gov/pubmed/24412899
http://dx.doi.org/10.3390/s140101028
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