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Phosphoglycerate dehydrogenase is dispensable for breast tumor maintenance and growth

Cancer cells rely on aerobic glycolysis to maintain cell growth and proliferation via the Warburg effect. Phosphoglycerate dehydrogenase (PHDGH) catalyzes the first step of the serine biosynthetic pathway downstream of glycolysis, which is a metabolic gatekeeper both for macromolecular biosynthesis...

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Autores principales: Chen, Jinyun, Chung, Franklin, Yang, Guizhi, Pu, Minying, Gao, Hui, Jiang, Wei, Yin, Hong, Capka, Vladimir, Kasibhatla, Shailaja, Laffitte, Bryan, Jaeger, Savina, Pagliarini, Raymond, Chen, Yaoyu, Zhou, Wenlai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3926844/
https://www.ncbi.nlm.nih.gov/pubmed/24318446
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author Chen, Jinyun
Chung, Franklin
Yang, Guizhi
Pu, Minying
Gao, Hui
Jiang, Wei
Yin, Hong
Capka, Vladimir
Kasibhatla, Shailaja
Laffitte, Bryan
Jaeger, Savina
Pagliarini, Raymond
Chen, Yaoyu
Zhou, Wenlai
author_facet Chen, Jinyun
Chung, Franklin
Yang, Guizhi
Pu, Minying
Gao, Hui
Jiang, Wei
Yin, Hong
Capka, Vladimir
Kasibhatla, Shailaja
Laffitte, Bryan
Jaeger, Savina
Pagliarini, Raymond
Chen, Yaoyu
Zhou, Wenlai
author_sort Chen, Jinyun
collection PubMed
description Cancer cells rely on aerobic glycolysis to maintain cell growth and proliferation via the Warburg effect. Phosphoglycerate dehydrogenase (PHDGH) catalyzes the first step of the serine biosynthetic pathway downstream of glycolysis, which is a metabolic gatekeeper both for macromolecular biosynthesis and serine-dependent DNA synthesis. Here, we report that PHDGH is overexpressed in many ER-negative human breast cancer cell lines. PHGDH knockdown in these cells leads to a reduction of serine synthesis and impairment of cancer cell proliferation. However, PHGDH knockdown does not affect tumor maintenance and growth in established breast cancer xenograft models, suggesting that PHGDH-dependent cancer cell growth may be context-dependent. Our findings suggest that other mechanisms or pathways may bypass exclusive dependence on PHGDH in established human breast cancer xenografts, indicating that PHGDH is dispensable for the growth and maintenance of tumors in vivo.
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spelling pubmed-39268442014-02-18 Phosphoglycerate dehydrogenase is dispensable for breast tumor maintenance and growth Chen, Jinyun Chung, Franklin Yang, Guizhi Pu, Minying Gao, Hui Jiang, Wei Yin, Hong Capka, Vladimir Kasibhatla, Shailaja Laffitte, Bryan Jaeger, Savina Pagliarini, Raymond Chen, Yaoyu Zhou, Wenlai Oncotarget Research Paper Cancer cells rely on aerobic glycolysis to maintain cell growth and proliferation via the Warburg effect. Phosphoglycerate dehydrogenase (PHDGH) catalyzes the first step of the serine biosynthetic pathway downstream of glycolysis, which is a metabolic gatekeeper both for macromolecular biosynthesis and serine-dependent DNA synthesis. Here, we report that PHDGH is overexpressed in many ER-negative human breast cancer cell lines. PHGDH knockdown in these cells leads to a reduction of serine synthesis and impairment of cancer cell proliferation. However, PHGDH knockdown does not affect tumor maintenance and growth in established breast cancer xenograft models, suggesting that PHGDH-dependent cancer cell growth may be context-dependent. Our findings suggest that other mechanisms or pathways may bypass exclusive dependence on PHGDH in established human breast cancer xenografts, indicating that PHGDH is dispensable for the growth and maintenance of tumors in vivo. Impact Journals LLC 2013-11-26 /pmc/articles/PMC3926844/ /pubmed/24318446 Text en Copyright: © 2013 Chen et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Chen, Jinyun
Chung, Franklin
Yang, Guizhi
Pu, Minying
Gao, Hui
Jiang, Wei
Yin, Hong
Capka, Vladimir
Kasibhatla, Shailaja
Laffitte, Bryan
Jaeger, Savina
Pagliarini, Raymond
Chen, Yaoyu
Zhou, Wenlai
Phosphoglycerate dehydrogenase is dispensable for breast tumor maintenance and growth
title Phosphoglycerate dehydrogenase is dispensable for breast tumor maintenance and growth
title_full Phosphoglycerate dehydrogenase is dispensable for breast tumor maintenance and growth
title_fullStr Phosphoglycerate dehydrogenase is dispensable for breast tumor maintenance and growth
title_full_unstemmed Phosphoglycerate dehydrogenase is dispensable for breast tumor maintenance and growth
title_short Phosphoglycerate dehydrogenase is dispensable for breast tumor maintenance and growth
title_sort phosphoglycerate dehydrogenase is dispensable for breast tumor maintenance and growth
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3926844/
https://www.ncbi.nlm.nih.gov/pubmed/24318446
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