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Phosphoglycerate dehydrogenase is dispensable for breast tumor maintenance and growth
Cancer cells rely on aerobic glycolysis to maintain cell growth and proliferation via the Warburg effect. Phosphoglycerate dehydrogenase (PHDGH) catalyzes the first step of the serine biosynthetic pathway downstream of glycolysis, which is a metabolic gatekeeper both for macromolecular biosynthesis...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3926844/ https://www.ncbi.nlm.nih.gov/pubmed/24318446 |
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author | Chen, Jinyun Chung, Franklin Yang, Guizhi Pu, Minying Gao, Hui Jiang, Wei Yin, Hong Capka, Vladimir Kasibhatla, Shailaja Laffitte, Bryan Jaeger, Savina Pagliarini, Raymond Chen, Yaoyu Zhou, Wenlai |
author_facet | Chen, Jinyun Chung, Franklin Yang, Guizhi Pu, Minying Gao, Hui Jiang, Wei Yin, Hong Capka, Vladimir Kasibhatla, Shailaja Laffitte, Bryan Jaeger, Savina Pagliarini, Raymond Chen, Yaoyu Zhou, Wenlai |
author_sort | Chen, Jinyun |
collection | PubMed |
description | Cancer cells rely on aerobic glycolysis to maintain cell growth and proliferation via the Warburg effect. Phosphoglycerate dehydrogenase (PHDGH) catalyzes the first step of the serine biosynthetic pathway downstream of glycolysis, which is a metabolic gatekeeper both for macromolecular biosynthesis and serine-dependent DNA synthesis. Here, we report that PHDGH is overexpressed in many ER-negative human breast cancer cell lines. PHGDH knockdown in these cells leads to a reduction of serine synthesis and impairment of cancer cell proliferation. However, PHGDH knockdown does not affect tumor maintenance and growth in established breast cancer xenograft models, suggesting that PHGDH-dependent cancer cell growth may be context-dependent. Our findings suggest that other mechanisms or pathways may bypass exclusive dependence on PHGDH in established human breast cancer xenografts, indicating that PHGDH is dispensable for the growth and maintenance of tumors in vivo. |
format | Online Article Text |
id | pubmed-3926844 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-39268442014-02-18 Phosphoglycerate dehydrogenase is dispensable for breast tumor maintenance and growth Chen, Jinyun Chung, Franklin Yang, Guizhi Pu, Minying Gao, Hui Jiang, Wei Yin, Hong Capka, Vladimir Kasibhatla, Shailaja Laffitte, Bryan Jaeger, Savina Pagliarini, Raymond Chen, Yaoyu Zhou, Wenlai Oncotarget Research Paper Cancer cells rely on aerobic glycolysis to maintain cell growth and proliferation via the Warburg effect. Phosphoglycerate dehydrogenase (PHDGH) catalyzes the first step of the serine biosynthetic pathway downstream of glycolysis, which is a metabolic gatekeeper both for macromolecular biosynthesis and serine-dependent DNA synthesis. Here, we report that PHDGH is overexpressed in many ER-negative human breast cancer cell lines. PHGDH knockdown in these cells leads to a reduction of serine synthesis and impairment of cancer cell proliferation. However, PHGDH knockdown does not affect tumor maintenance and growth in established breast cancer xenograft models, suggesting that PHGDH-dependent cancer cell growth may be context-dependent. Our findings suggest that other mechanisms or pathways may bypass exclusive dependence on PHGDH in established human breast cancer xenografts, indicating that PHGDH is dispensable for the growth and maintenance of tumors in vivo. Impact Journals LLC 2013-11-26 /pmc/articles/PMC3926844/ /pubmed/24318446 Text en Copyright: © 2013 Chen et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Chen, Jinyun Chung, Franklin Yang, Guizhi Pu, Minying Gao, Hui Jiang, Wei Yin, Hong Capka, Vladimir Kasibhatla, Shailaja Laffitte, Bryan Jaeger, Savina Pagliarini, Raymond Chen, Yaoyu Zhou, Wenlai Phosphoglycerate dehydrogenase is dispensable for breast tumor maintenance and growth |
title | Phosphoglycerate dehydrogenase is dispensable for breast tumor maintenance and growth |
title_full | Phosphoglycerate dehydrogenase is dispensable for breast tumor maintenance and growth |
title_fullStr | Phosphoglycerate dehydrogenase is dispensable for breast tumor maintenance and growth |
title_full_unstemmed | Phosphoglycerate dehydrogenase is dispensable for breast tumor maintenance and growth |
title_short | Phosphoglycerate dehydrogenase is dispensable for breast tumor maintenance and growth |
title_sort | phosphoglycerate dehydrogenase is dispensable for breast tumor maintenance and growth |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3926844/ https://www.ncbi.nlm.nih.gov/pubmed/24318446 |
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