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The strength of SMAD1/5 activity determines the mode of stem cell division in the developing spinal cord

The different modes of stem cell division are tightly regulated to balance growth and differentiation during organ development and homeostasis. However, the mechanisms controlling such events are not fully understood. We have developed markers that provide the single cell resolution necessary to ide...

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Detalles Bibliográficos
Autores principales: Le Dréau, Gwenvael, Saade, Murielle, Gutiérrez-Vallejo, Irene, Martí, Elisa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3926951/
https://www.ncbi.nlm.nih.gov/pubmed/24515346
http://dx.doi.org/10.1083/jcb.201307031
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author Le Dréau, Gwenvael
Saade, Murielle
Gutiérrez-Vallejo, Irene
Martí, Elisa
author_facet Le Dréau, Gwenvael
Saade, Murielle
Gutiérrez-Vallejo, Irene
Martí, Elisa
author_sort Le Dréau, Gwenvael
collection PubMed
description The different modes of stem cell division are tightly regulated to balance growth and differentiation during organ development and homeostasis. However, the mechanisms controlling such events are not fully understood. We have developed markers that provide the single cell resolution necessary to identify the three modes of division occurring in a developing nervous system: self-expanding, self-renewing, and self-consuming. Characterizing these three modes of division during interneuron generation in the developing chick spinal cord, we demonstrated that they correlate to different levels of activity of the canonical bone morphogenetic protein effectors SMAD1/5. Functional in vivo experiments showed that the premature neuronal differentiation and changes in cell cycle parameters caused by SMAD1/5 inhibition were preceded by a reduction of self-expanding divisions in favor of self-consuming divisions. Conversely, SMAD1/5 gain of function promoted self-expanding divisions. Together, these results lead us to propose that the strength of SMAD1/5 activity dictates the mode of stem cell division during spinal interneuron generation.
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spelling pubmed-39269512014-08-17 The strength of SMAD1/5 activity determines the mode of stem cell division in the developing spinal cord Le Dréau, Gwenvael Saade, Murielle Gutiérrez-Vallejo, Irene Martí, Elisa J Cell Biol Research Articles The different modes of stem cell division are tightly regulated to balance growth and differentiation during organ development and homeostasis. However, the mechanisms controlling such events are not fully understood. We have developed markers that provide the single cell resolution necessary to identify the three modes of division occurring in a developing nervous system: self-expanding, self-renewing, and self-consuming. Characterizing these three modes of division during interneuron generation in the developing chick spinal cord, we demonstrated that they correlate to different levels of activity of the canonical bone morphogenetic protein effectors SMAD1/5. Functional in vivo experiments showed that the premature neuronal differentiation and changes in cell cycle parameters caused by SMAD1/5 inhibition were preceded by a reduction of self-expanding divisions in favor of self-consuming divisions. Conversely, SMAD1/5 gain of function promoted self-expanding divisions. Together, these results lead us to propose that the strength of SMAD1/5 activity dictates the mode of stem cell division during spinal interneuron generation. The Rockefeller University Press 2014-02-17 /pmc/articles/PMC3926951/ /pubmed/24515346 http://dx.doi.org/10.1083/jcb.201307031 Text en © 2014 Le Dréau et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Le Dréau, Gwenvael
Saade, Murielle
Gutiérrez-Vallejo, Irene
Martí, Elisa
The strength of SMAD1/5 activity determines the mode of stem cell division in the developing spinal cord
title The strength of SMAD1/5 activity determines the mode of stem cell division in the developing spinal cord
title_full The strength of SMAD1/5 activity determines the mode of stem cell division in the developing spinal cord
title_fullStr The strength of SMAD1/5 activity determines the mode of stem cell division in the developing spinal cord
title_full_unstemmed The strength of SMAD1/5 activity determines the mode of stem cell division in the developing spinal cord
title_short The strength of SMAD1/5 activity determines the mode of stem cell division in the developing spinal cord
title_sort strength of smad1/5 activity determines the mode of stem cell division in the developing spinal cord
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3926951/
https://www.ncbi.nlm.nih.gov/pubmed/24515346
http://dx.doi.org/10.1083/jcb.201307031
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