Development of a quantitative Correlative Light Electron Microscopy technique to study GLUT4 trafficking

Correlative Light Electron Microscopy (CLEM) combines advantages of light microscopy and electron microscopy in one experiment to deliver information above and beyond the capability of either modality alone. There are many different CLEM techniques, each having its own special advantages but also it...

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Detalles Bibliográficos
Autores principales: Hodgson, Lorna, Tavaré, Jeremy, Verkade, Paul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2014
Materias:
Special Issue: New/Emerging Techniques in Biological Microscopy
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3927059/
https://www.ncbi.nlm.nih.gov/pubmed/24390248
http://dx.doi.org/10.1007/s00709-013-0597-5
Descripción
Sumario:Correlative Light Electron Microscopy (CLEM) combines advantages of light microscopy and electron microscopy in one experiment to deliver information above and beyond the capability of either modality alone. There are many different CLEM techniques, each having its own special advantages but also its technical challenges. It is however the biological question that (should) drive(s) the development and application of a specific CLEM technique in order to provide the answer. Here we describe the development of a CLEM technique that is based on the Tokuyasu cryo immuno-gold labelling technique that has allowed us to quantitatively study GLUT4 trafficking.

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