Development of a quantitative Correlative Light Electron Microscopy technique to study GLUT4 trafficking

Correlative Light Electron Microscopy (CLEM) combines advantages of light microscopy and electron microscopy in one experiment to deliver information above and beyond the capability of either modality alone. There are many different CLEM techniques, each having its own special advantages but also it...

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Detalles Bibliográficos
Autores principales: Hodgson, Lorna, Tavaré, Jeremy, Verkade, Paul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2014
Materias:
Special Issue: New/Emerging Techniques in Biological Microscopy
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3927059/
https://www.ncbi.nlm.nih.gov/pubmed/24390248
http://dx.doi.org/10.1007/s00709-013-0597-5
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author Hodgson, Lorna
Tavaré, Jeremy
Verkade, Paul
author_facet Hodgson, Lorna
Tavaré, Jeremy
Verkade, Paul
author_sort Hodgson, Lorna
collection PubMed
description Correlative Light Electron Microscopy (CLEM) combines advantages of light microscopy and electron microscopy in one experiment to deliver information above and beyond the capability of either modality alone. There are many different CLEM techniques, each having its own special advantages but also its technical challenges. It is however the biological question that (should) drive(s) the development and application of a specific CLEM technique in order to provide the answer. Here we describe the development of a CLEM technique that is based on the Tokuyasu cryo immuno-gold labelling technique that has allowed us to quantitatively study GLUT4 trafficking.
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spelling pubmed-39270592014-02-21 Development of a quantitative Correlative Light Electron Microscopy technique to study GLUT4 trafficking Hodgson, Lorna Tavaré, Jeremy Verkade, Paul Protoplasma Special Issue: New/Emerging Techniques in Biological Microscopy Correlative Light Electron Microscopy (CLEM) combines advantages of light microscopy and electron microscopy in one experiment to deliver information above and beyond the capability of either modality alone. There are many different CLEM techniques, each having its own special advantages but also its technical challenges. It is however the biological question that (should) drive(s) the development and application of a specific CLEM technique in order to provide the answer. Here we describe the development of a CLEM technique that is based on the Tokuyasu cryo immuno-gold labelling technique that has allowed us to quantitatively study GLUT4 trafficking. Springer Vienna 2014-01-04 2014 /pmc/articles/PMC3927059/ /pubmed/24390248 http://dx.doi.org/10.1007/s00709-013-0597-5 Text en © The Author(s) 2014 https://creativecommons.org/licenses/by/2.0/ Open Access This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Special Issue: New/Emerging Techniques in Biological Microscopy
Hodgson, Lorna
Tavaré, Jeremy
Verkade, Paul
Development of a quantitative Correlative Light Electron Microscopy technique to study GLUT4 trafficking
title Development of a quantitative Correlative Light Electron Microscopy technique to study GLUT4 trafficking
title_full Development of a quantitative Correlative Light Electron Microscopy technique to study GLUT4 trafficking
title_fullStr Development of a quantitative Correlative Light Electron Microscopy technique to study GLUT4 trafficking
title_full_unstemmed Development of a quantitative Correlative Light Electron Microscopy technique to study GLUT4 trafficking
title_short Development of a quantitative Correlative Light Electron Microscopy technique to study GLUT4 trafficking
title_sort development of a quantitative correlative light electron microscopy technique to study glut4 trafficking
topic Special Issue: New/Emerging Techniques in Biological Microscopy
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3927059/
https://www.ncbi.nlm.nih.gov/pubmed/24390248
http://dx.doi.org/10.1007/s00709-013-0597-5
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