Molecular characterization of Toxoplasma gondii Type II in sheep abortion in Sardinia, Italy

During 2009–2010, 161 tissue samples (142 placentas, 16 brains, and 3 livers) from aborted ovine fetuses on Sardinia Island, Italy, were tested for toxoplasmosis. Organs that showed a positive result by nested polymerase chain reaction (PCR) targeting the ITS1 region for Toxoplasma gondii were also...

Descripción completa

Detalles Bibliográficos
Autores principales: Chessa, Giovanna, Chisu, Valentina, Porcu, Rosaura, Masala, Giovanna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: EDP Sciences 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3927306/
https://www.ncbi.nlm.nih.gov/pubmed/24534616
http://dx.doi.org/10.1051/parasite/2014007
Descripción
Sumario:During 2009–2010, 161 tissue samples (142 placentas, 16 brains, and 3 livers) from aborted ovine fetuses on Sardinia Island, Italy, were tested for toxoplasmosis. Organs that showed a positive result by nested polymerase chain reaction (PCR) targeting the ITS1 region for Toxoplasma gondii were also amplified with 11 genetic markers (SAG1, 5′-SAG2, 3′-SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and Apico) and then subjected to PCR/RFLP for genetic typing. T. gondii DNA was found in 5 placentas, 14 brains, and 2 livers by PCR analysis and all isolates displayed Type II alleles at all 11 loci with all 11 markers. The results indicate that the Type II T. gondii is associated with ovine abortion.

Ejemplares similares