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In vitro transfection of bone marrow-derived dendritic cells with TATp-liposomes
Dendritic cells (DC) are antigen-presenting cells uniquely capable of priming naïve T cells and cross-presenting antigens, and they determine the type of immune response elicited against an antigen. TAT peptide (TATp), is an amphipathic, arginine-rich, cationic peptide that promotes penetration and...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3928453/ https://www.ncbi.nlm.nih.gov/pubmed/24611012 http://dx.doi.org/10.2147/IJN.S53432 |
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author | Pappalardo, Juan Sebastián Langellotti, Cecilia A Di Giacomo, Sebastián Olivera, Valeria Quattrocchi, Valeria Zamorano, Patricia I Hartner, William C Levchenko, Tatyana S Torchilin, Vladimir P |
author_facet | Pappalardo, Juan Sebastián Langellotti, Cecilia A Di Giacomo, Sebastián Olivera, Valeria Quattrocchi, Valeria Zamorano, Patricia I Hartner, William C Levchenko, Tatyana S Torchilin, Vladimir P |
author_sort | Pappalardo, Juan Sebastián |
collection | PubMed |
description | Dendritic cells (DC) are antigen-presenting cells uniquely capable of priming naïve T cells and cross-presenting antigens, and they determine the type of immune response elicited against an antigen. TAT peptide (TATp), is an amphipathic, arginine-rich, cationic peptide that promotes penetration and translocation of various molecules and nanoparticles into cells. TATp-liposomes (TATp-L) used for DC transfection were prepared using TATp derivatized with a lipid-terminated polymer capable of anchoring in the liposomal membrane. Here, we show that the addition of TATp to DNA-loaded liposomes increased the uptake of DNA in DC. DNA-loaded TATp-L increased the in vitro transfection efficiency in DC cultures as evidenced by a higher expression of the enhanced green fluorescent protein and bovine herpes virus type 1 glycoprotein D (gD). The de novo synthesized gD protein was immunologically stimulating when transfections were performed with TATp-L, as indicated by the secretion of interleukin 6. |
format | Online Article Text |
id | pubmed-3928453 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-39284532014-03-07 In vitro transfection of bone marrow-derived dendritic cells with TATp-liposomes Pappalardo, Juan Sebastián Langellotti, Cecilia A Di Giacomo, Sebastián Olivera, Valeria Quattrocchi, Valeria Zamorano, Patricia I Hartner, William C Levchenko, Tatyana S Torchilin, Vladimir P Int J Nanomedicine Original Research Dendritic cells (DC) are antigen-presenting cells uniquely capable of priming naïve T cells and cross-presenting antigens, and they determine the type of immune response elicited against an antigen. TAT peptide (TATp), is an amphipathic, arginine-rich, cationic peptide that promotes penetration and translocation of various molecules and nanoparticles into cells. TATp-liposomes (TATp-L) used for DC transfection were prepared using TATp derivatized with a lipid-terminated polymer capable of anchoring in the liposomal membrane. Here, we show that the addition of TATp to DNA-loaded liposomes increased the uptake of DNA in DC. DNA-loaded TATp-L increased the in vitro transfection efficiency in DC cultures as evidenced by a higher expression of the enhanced green fluorescent protein and bovine herpes virus type 1 glycoprotein D (gD). The de novo synthesized gD protein was immunologically stimulating when transfections were performed with TATp-L, as indicated by the secretion of interleukin 6. Dove Medical Press 2014-02-13 /pmc/articles/PMC3928453/ /pubmed/24611012 http://dx.doi.org/10.2147/IJN.S53432 Text en © 2014 Pappalardo et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. |
spellingShingle | Original Research Pappalardo, Juan Sebastián Langellotti, Cecilia A Di Giacomo, Sebastián Olivera, Valeria Quattrocchi, Valeria Zamorano, Patricia I Hartner, William C Levchenko, Tatyana S Torchilin, Vladimir P In vitro transfection of bone marrow-derived dendritic cells with TATp-liposomes |
title | In vitro transfection of bone marrow-derived dendritic cells with TATp-liposomes |
title_full | In vitro transfection of bone marrow-derived dendritic cells with TATp-liposomes |
title_fullStr | In vitro transfection of bone marrow-derived dendritic cells with TATp-liposomes |
title_full_unstemmed | In vitro transfection of bone marrow-derived dendritic cells with TATp-liposomes |
title_short | In vitro transfection of bone marrow-derived dendritic cells with TATp-liposomes |
title_sort | in vitro transfection of bone marrow-derived dendritic cells with tatp-liposomes |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3928453/ https://www.ncbi.nlm.nih.gov/pubmed/24611012 http://dx.doi.org/10.2147/IJN.S53432 |
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