GLI1 upregulates C-JUN through a specific 130-kDa isoform

The Hedgehog pathway is molecularly linked to increased resistance to cisplatin and increased repair of platinum-DNA damage, through C-JUN. GLI1, which has five known isoforms, is a positive transcriptional regulator in Hedgehog. Southwestern blot assay, EMSA and ChIP assays indicate that only one o...

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Detalles Bibliográficos
Autores principales: AMABLE, LAUREN, GAVIN, ELAINE, KUDO, KENJI, MENG, ERHONG, ROCCONI, RODNEY P., SHEVDE, LALITA A., REED, EDDIE
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2013
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3928471/
https://www.ncbi.nlm.nih.gov/pubmed/24366538
http://dx.doi.org/10.3892/ijo.2013.2222
Descripción
Sumario:The Hedgehog pathway is molecularly linked to increased resistance to cisplatin and increased repair of platinum-DNA damage, through C-JUN. GLI1, which has five known isoforms, is a positive transcriptional regulator in Hedgehog. Southwestern blot assay, EMSA and ChIP assays indicate that only one of five isoforms of GLI1 may be responsible for the Hedgehog link with C-JUN and thus, increased platinum-DNA adduct repair. Cancer tissues express this 130-kDa isoform at levels 6-fold higher than non-malignant tissues; and this isoform exists in abundance in six of seven ovarian cancer cell lines examined.

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