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Chronic Ethanol Feeding Modulates Inflammatory Mediators, Activation of Nuclear Factor-κB, and Responsiveness to Endotoxin in Murine Kupffer Cells and Circulating Leukocytes

Chronic ethanol abuse is known to increase susceptibility to infections after injury, in part, by modification of macrophage function. Several intracellular signalling mechanisms are involved in the initiation of inflammatory responses, including the nuclear factor-κB (NF-κB) pathway. In this study,...

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Autores principales: Maraslioglu, Miriam, Oppermann, Elsie, Blattner, Carolin, Weber, Roxane, Henrich, Dirk, Jobin, Christian, Schleucher, Elke, Marzi, Ingo, Lehnert, Mark
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3928853/
https://www.ncbi.nlm.nih.gov/pubmed/24623963
http://dx.doi.org/10.1155/2014/808695
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author Maraslioglu, Miriam
Oppermann, Elsie
Blattner, Carolin
Weber, Roxane
Henrich, Dirk
Jobin, Christian
Schleucher, Elke
Marzi, Ingo
Lehnert, Mark
author_facet Maraslioglu, Miriam
Oppermann, Elsie
Blattner, Carolin
Weber, Roxane
Henrich, Dirk
Jobin, Christian
Schleucher, Elke
Marzi, Ingo
Lehnert, Mark
author_sort Maraslioglu, Miriam
collection PubMed
description Chronic ethanol abuse is known to increase susceptibility to infections after injury, in part, by modification of macrophage function. Several intracellular signalling mechanisms are involved in the initiation of inflammatory responses, including the nuclear factor-κB (NF-κB) pathway. In this study, we investigated the systemic and hepatic effect of chronic ethanol feeding on in vivo activation of NF-κB in NF-κB(EGFP) reporter gene mice. Specifically, the study focused on Kupffer cell proinflammatory cytokines IL-6 and TNF-α and activation of NF-κB after chronic ethanol feeding followed by in vitro stimulation with lipopolysaccharide (LPS). We found that chronic ethanol upregulated NF-κB activation and increased hepatic and systemic proinflammatory cytokine levels. Similarly, LPS-stimulated IL-1β release from whole blood was significantly enhanced in ethanol-fed mice. However, LPS significantly increased IL-6 and TNF-α levels. These results demonstrate that chronic ethanol feeding can improve the responsiveness of macrophage LPS-stimulated IL-6 and TNF-α production and indicate that this effect may result from ethanol-induced alterations in intracellular signalling through NF-κB. Furthermore, LPS and TNF-α stimulated the gene expression of different inflammatory mediators, in part, in a NF-κB-dependent manner.
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spelling pubmed-39288532014-03-12 Chronic Ethanol Feeding Modulates Inflammatory Mediators, Activation of Nuclear Factor-κB, and Responsiveness to Endotoxin in Murine Kupffer Cells and Circulating Leukocytes Maraslioglu, Miriam Oppermann, Elsie Blattner, Carolin Weber, Roxane Henrich, Dirk Jobin, Christian Schleucher, Elke Marzi, Ingo Lehnert, Mark Mediators Inflamm Research Article Chronic ethanol abuse is known to increase susceptibility to infections after injury, in part, by modification of macrophage function. Several intracellular signalling mechanisms are involved in the initiation of inflammatory responses, including the nuclear factor-κB (NF-κB) pathway. In this study, we investigated the systemic and hepatic effect of chronic ethanol feeding on in vivo activation of NF-κB in NF-κB(EGFP) reporter gene mice. Specifically, the study focused on Kupffer cell proinflammatory cytokines IL-6 and TNF-α and activation of NF-κB after chronic ethanol feeding followed by in vitro stimulation with lipopolysaccharide (LPS). We found that chronic ethanol upregulated NF-κB activation and increased hepatic and systemic proinflammatory cytokine levels. Similarly, LPS-stimulated IL-1β release from whole blood was significantly enhanced in ethanol-fed mice. However, LPS significantly increased IL-6 and TNF-α levels. These results demonstrate that chronic ethanol feeding can improve the responsiveness of macrophage LPS-stimulated IL-6 and TNF-α production and indicate that this effect may result from ethanol-induced alterations in intracellular signalling through NF-κB. Furthermore, LPS and TNF-α stimulated the gene expression of different inflammatory mediators, in part, in a NF-κB-dependent manner. Hindawi Publishing Corporation 2014 2014-01-29 /pmc/articles/PMC3928853/ /pubmed/24623963 http://dx.doi.org/10.1155/2014/808695 Text en Copyright © 2014 Miriam Maraslioglu et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Maraslioglu, Miriam
Oppermann, Elsie
Blattner, Carolin
Weber, Roxane
Henrich, Dirk
Jobin, Christian
Schleucher, Elke
Marzi, Ingo
Lehnert, Mark
Chronic Ethanol Feeding Modulates Inflammatory Mediators, Activation of Nuclear Factor-κB, and Responsiveness to Endotoxin in Murine Kupffer Cells and Circulating Leukocytes
title Chronic Ethanol Feeding Modulates Inflammatory Mediators, Activation of Nuclear Factor-κB, and Responsiveness to Endotoxin in Murine Kupffer Cells and Circulating Leukocytes
title_full Chronic Ethanol Feeding Modulates Inflammatory Mediators, Activation of Nuclear Factor-κB, and Responsiveness to Endotoxin in Murine Kupffer Cells and Circulating Leukocytes
title_fullStr Chronic Ethanol Feeding Modulates Inflammatory Mediators, Activation of Nuclear Factor-κB, and Responsiveness to Endotoxin in Murine Kupffer Cells and Circulating Leukocytes
title_full_unstemmed Chronic Ethanol Feeding Modulates Inflammatory Mediators, Activation of Nuclear Factor-κB, and Responsiveness to Endotoxin in Murine Kupffer Cells and Circulating Leukocytes
title_short Chronic Ethanol Feeding Modulates Inflammatory Mediators, Activation of Nuclear Factor-κB, and Responsiveness to Endotoxin in Murine Kupffer Cells and Circulating Leukocytes
title_sort chronic ethanol feeding modulates inflammatory mediators, activation of nuclear factor-κb, and responsiveness to endotoxin in murine kupffer cells and circulating leukocytes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3928853/
https://www.ncbi.nlm.nih.gov/pubmed/24623963
http://dx.doi.org/10.1155/2014/808695
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