Cargando…
Effect of Selenate on Viability and Selenomethionine Accumulation of Chlorella sorokiniana Grown in Batch Culture
The aim of this work was to study the effect of Se(+VI) on viability, cell morphology, and selenomethionine accumulation of the green alga Chlorella sorokiniana grown in batch cultures. Culture exposed to sublethal Se concentrations of 40 mg·L(−1) (212 μM) decreased growth rates for about 25% compar...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3928859/ https://www.ncbi.nlm.nih.gov/pubmed/24688385 http://dx.doi.org/10.1155/2014/401265 |
_version_ | 1782304324319182848 |
---|---|
author | Gojkovic, Živan Vílchez, Carlos Torronteras, Rafael Vigara, Javier Gómez-Jacinto, Veronica Janzer, Nora Gómez-Ariza, José-Luis Márová, Ivana Garbayo, Ines |
author_facet | Gojkovic, Živan Vílchez, Carlos Torronteras, Rafael Vigara, Javier Gómez-Jacinto, Veronica Janzer, Nora Gómez-Ariza, José-Luis Márová, Ivana Garbayo, Ines |
author_sort | Gojkovic, Živan |
collection | PubMed |
description | The aim of this work was to study the effect of Se(+VI) on viability, cell morphology, and selenomethionine accumulation of the green alga Chlorella sorokiniana grown in batch cultures. Culture exposed to sublethal Se concentrations of 40 mg·L(−1) (212 μM) decreased growth rates for about 25% compared to control. A selenate EC(50) value of 45 mg·L(−1) (238.2 μM) was determined. Results showed that chlorophyll and carotenoids contents were not affected by Se exposure, while oxygen evolution decreased by half. Ultrastructural studies revealed granular stroma, fingerprint-like appearance of thylakoids which did not compromise cell activity. Unlike control cultures, SDS PAGE electrophoresis of crude extracts from selenate-exposed cell cultures revealed appearance of a protein band identified as 53 kDa Rubisco large subunit of Chlorella sorokiniana, suggesting that selenate affects expression of the corresponding chloroplast gene as this subunit is encoded in the chloroplast DNA. Results revealed that the microalga was able to accumulate up to 140 mg·kg(−1) of SeMet in 120 h of cultivation. This paper shows that Chlorella sorokiniana biomass can be enriched in the high value aminoacid SeMet in batch cultures, while keeping photochemical viability and carbon dioxide fixation activity intact, if exposed to suitable sublethal concentrations of Se. |
format | Online Article Text |
id | pubmed-3928859 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-39288592014-03-31 Effect of Selenate on Viability and Selenomethionine Accumulation of Chlorella sorokiniana Grown in Batch Culture Gojkovic, Živan Vílchez, Carlos Torronteras, Rafael Vigara, Javier Gómez-Jacinto, Veronica Janzer, Nora Gómez-Ariza, José-Luis Márová, Ivana Garbayo, Ines ScientificWorldJournal Research Article The aim of this work was to study the effect of Se(+VI) on viability, cell morphology, and selenomethionine accumulation of the green alga Chlorella sorokiniana grown in batch cultures. Culture exposed to sublethal Se concentrations of 40 mg·L(−1) (212 μM) decreased growth rates for about 25% compared to control. A selenate EC(50) value of 45 mg·L(−1) (238.2 μM) was determined. Results showed that chlorophyll and carotenoids contents were not affected by Se exposure, while oxygen evolution decreased by half. Ultrastructural studies revealed granular stroma, fingerprint-like appearance of thylakoids which did not compromise cell activity. Unlike control cultures, SDS PAGE electrophoresis of crude extracts from selenate-exposed cell cultures revealed appearance of a protein band identified as 53 kDa Rubisco large subunit of Chlorella sorokiniana, suggesting that selenate affects expression of the corresponding chloroplast gene as this subunit is encoded in the chloroplast DNA. Results revealed that the microalga was able to accumulate up to 140 mg·kg(−1) of SeMet in 120 h of cultivation. This paper shows that Chlorella sorokiniana biomass can be enriched in the high value aminoacid SeMet in batch cultures, while keeping photochemical viability and carbon dioxide fixation activity intact, if exposed to suitable sublethal concentrations of Se. Hindawi Publishing Corporation 2014-01-29 /pmc/articles/PMC3928859/ /pubmed/24688385 http://dx.doi.org/10.1155/2014/401265 Text en Copyright © 2014 Živan Gojkovic et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Gojkovic, Živan Vílchez, Carlos Torronteras, Rafael Vigara, Javier Gómez-Jacinto, Veronica Janzer, Nora Gómez-Ariza, José-Luis Márová, Ivana Garbayo, Ines Effect of Selenate on Viability and Selenomethionine Accumulation of Chlorella sorokiniana Grown in Batch Culture |
title | Effect of Selenate on Viability and Selenomethionine Accumulation of Chlorella sorokiniana Grown in Batch Culture |
title_full | Effect of Selenate on Viability and Selenomethionine Accumulation of Chlorella sorokiniana Grown in Batch Culture |
title_fullStr | Effect of Selenate on Viability and Selenomethionine Accumulation of Chlorella sorokiniana Grown in Batch Culture |
title_full_unstemmed | Effect of Selenate on Viability and Selenomethionine Accumulation of Chlorella sorokiniana Grown in Batch Culture |
title_short | Effect of Selenate on Viability and Selenomethionine Accumulation of Chlorella sorokiniana Grown in Batch Culture |
title_sort | effect of selenate on viability and selenomethionine accumulation of chlorella sorokiniana grown in batch culture |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3928859/ https://www.ncbi.nlm.nih.gov/pubmed/24688385 http://dx.doi.org/10.1155/2014/401265 |
work_keys_str_mv | AT gojkoviczivan effectofselenateonviabilityandselenomethionineaccumulationofchlorellasorokinianagrowninbatchculture AT vilchezcarlos effectofselenateonviabilityandselenomethionineaccumulationofchlorellasorokinianagrowninbatchculture AT torronterasrafael effectofselenateonviabilityandselenomethionineaccumulationofchlorellasorokinianagrowninbatchculture AT vigarajavier effectofselenateonviabilityandselenomethionineaccumulationofchlorellasorokinianagrowninbatchculture AT gomezjacintoveronica effectofselenateonviabilityandselenomethionineaccumulationofchlorellasorokinianagrowninbatchculture AT janzernora effectofselenateonviabilityandselenomethionineaccumulationofchlorellasorokinianagrowninbatchculture AT gomezarizajoseluis effectofselenateonviabilityandselenomethionineaccumulationofchlorellasorokinianagrowninbatchculture AT marovaivana effectofselenateonviabilityandselenomethionineaccumulationofchlorellasorokinianagrowninbatchculture AT garbayoines effectofselenateonviabilityandselenomethionineaccumulationofchlorellasorokinianagrowninbatchculture |