Cargando…
Protective effects of hydrogen enriched saline on liver ischemia reperfusion injury by reducing oxidative stress and HMGB1 release
BACKGROUND: The nuclear protein high-mobility group box 1 (HMGB1) is a key trigger for the inflammatory reaction during liver ischemia reperfusion injury (IRI). Hydrogen treatment was recently associated with down-regulation of the expression of HMGB1 and pro-inflammatory cytokines during sepsis and...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3928909/ https://www.ncbi.nlm.nih.gov/pubmed/24410860 http://dx.doi.org/10.1186/1471-230X-14-12 |
_version_ | 1782304330499489792 |
---|---|
author | Liu, Yantao Yang, Liqun Tao, Kunming Vizcaychipi, Marcela P Lloyd, Dafydd M Sun, Xuejun Irwin, Michael G Ma, Daqing Yu, Weifeng |
author_facet | Liu, Yantao Yang, Liqun Tao, Kunming Vizcaychipi, Marcela P Lloyd, Dafydd M Sun, Xuejun Irwin, Michael G Ma, Daqing Yu, Weifeng |
author_sort | Liu, Yantao |
collection | PubMed |
description | BACKGROUND: The nuclear protein high-mobility group box 1 (HMGB1) is a key trigger for the inflammatory reaction during liver ischemia reperfusion injury (IRI). Hydrogen treatment was recently associated with down-regulation of the expression of HMGB1 and pro-inflammatory cytokines during sepsis and myocardial IRI, but it is not known whether hydrogen has an effect on HMGB1 in liver IRI. METHODS: A rat model of 60 minutes 70% partial liver ischemia reperfusion injury was used. Hydrogen enriched saline (2.5, 5 or 10 ml/kg) was injected intraperitoneally 10 minutes before hepatic reperfusion. Liver injury was assessed by serum alanine aminotransferase (ALT) enzyme levels and histological changes. We also measured malondialdehyde (MDA), hydroxynonenal (HNE) and 8-hydroxy-guanosine (8-OH-G) levels as markers of the peroxidation injury induced by reactive oxygen species (ROS). In addition, pro-inflammatory cytokines including TNF-α and IL-6, and high mobility group box B1 protein (HMGB1) were measured as markers of post ischemia-reperfusion inflammation. RESULTS: Hydrogen enriched saline treatment significantly attenuated the severity of liver injury induced by ischemia-reperfusion. The treatment group showed reduced serum ALT activity and markers of lipid peroxidation and post ischemia reperfusion histological changes were reduced. Hydrogen enriched saline treatment inhibited HMGB1 expression and release, reflecting a reduced local and systemic inflammatory response to hepatic ischemia reperfusion. CONCLUSION: These results suggest that, in our model, hydrogen enriched saline treatment is protective against liver ischemia-reperfusion injury. This effect may be mediated by both the anti-oxidative and anti-inflammatory effects of the solution. |
format | Online Article Text |
id | pubmed-3928909 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-39289092014-02-20 Protective effects of hydrogen enriched saline on liver ischemia reperfusion injury by reducing oxidative stress and HMGB1 release Liu, Yantao Yang, Liqun Tao, Kunming Vizcaychipi, Marcela P Lloyd, Dafydd M Sun, Xuejun Irwin, Michael G Ma, Daqing Yu, Weifeng BMC Gastroenterol Research Article BACKGROUND: The nuclear protein high-mobility group box 1 (HMGB1) is a key trigger for the inflammatory reaction during liver ischemia reperfusion injury (IRI). Hydrogen treatment was recently associated with down-regulation of the expression of HMGB1 and pro-inflammatory cytokines during sepsis and myocardial IRI, but it is not known whether hydrogen has an effect on HMGB1 in liver IRI. METHODS: A rat model of 60 minutes 70% partial liver ischemia reperfusion injury was used. Hydrogen enriched saline (2.5, 5 or 10 ml/kg) was injected intraperitoneally 10 minutes before hepatic reperfusion. Liver injury was assessed by serum alanine aminotransferase (ALT) enzyme levels and histological changes. We also measured malondialdehyde (MDA), hydroxynonenal (HNE) and 8-hydroxy-guanosine (8-OH-G) levels as markers of the peroxidation injury induced by reactive oxygen species (ROS). In addition, pro-inflammatory cytokines including TNF-α and IL-6, and high mobility group box B1 protein (HMGB1) were measured as markers of post ischemia-reperfusion inflammation. RESULTS: Hydrogen enriched saline treatment significantly attenuated the severity of liver injury induced by ischemia-reperfusion. The treatment group showed reduced serum ALT activity and markers of lipid peroxidation and post ischemia reperfusion histological changes were reduced. Hydrogen enriched saline treatment inhibited HMGB1 expression and release, reflecting a reduced local and systemic inflammatory response to hepatic ischemia reperfusion. CONCLUSION: These results suggest that, in our model, hydrogen enriched saline treatment is protective against liver ischemia-reperfusion injury. This effect may be mediated by both the anti-oxidative and anti-inflammatory effects of the solution. BioMed Central 2014-01-12 /pmc/articles/PMC3928909/ /pubmed/24410860 http://dx.doi.org/10.1186/1471-230X-14-12 Text en Copyright © 2014 Liu et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Liu, Yantao Yang, Liqun Tao, Kunming Vizcaychipi, Marcela P Lloyd, Dafydd M Sun, Xuejun Irwin, Michael G Ma, Daqing Yu, Weifeng Protective effects of hydrogen enriched saline on liver ischemia reperfusion injury by reducing oxidative stress and HMGB1 release |
title | Protective effects of hydrogen enriched saline on liver ischemia reperfusion injury by reducing oxidative stress and HMGB1 release |
title_full | Protective effects of hydrogen enriched saline on liver ischemia reperfusion injury by reducing oxidative stress and HMGB1 release |
title_fullStr | Protective effects of hydrogen enriched saline on liver ischemia reperfusion injury by reducing oxidative stress and HMGB1 release |
title_full_unstemmed | Protective effects of hydrogen enriched saline on liver ischemia reperfusion injury by reducing oxidative stress and HMGB1 release |
title_short | Protective effects of hydrogen enriched saline on liver ischemia reperfusion injury by reducing oxidative stress and HMGB1 release |
title_sort | protective effects of hydrogen enriched saline on liver ischemia reperfusion injury by reducing oxidative stress and hmgb1 release |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3928909/ https://www.ncbi.nlm.nih.gov/pubmed/24410860 http://dx.doi.org/10.1186/1471-230X-14-12 |
work_keys_str_mv | AT liuyantao protectiveeffectsofhydrogenenrichedsalineonliverischemiareperfusioninjurybyreducingoxidativestressandhmgb1release AT yangliqun protectiveeffectsofhydrogenenrichedsalineonliverischemiareperfusioninjurybyreducingoxidativestressandhmgb1release AT taokunming protectiveeffectsofhydrogenenrichedsalineonliverischemiareperfusioninjurybyreducingoxidativestressandhmgb1release AT vizcaychipimarcelap protectiveeffectsofhydrogenenrichedsalineonliverischemiareperfusioninjurybyreducingoxidativestressandhmgb1release AT lloyddafyddm protectiveeffectsofhydrogenenrichedsalineonliverischemiareperfusioninjurybyreducingoxidativestressandhmgb1release AT sunxuejun protectiveeffectsofhydrogenenrichedsalineonliverischemiareperfusioninjurybyreducingoxidativestressandhmgb1release AT irwinmichaelg protectiveeffectsofhydrogenenrichedsalineonliverischemiareperfusioninjurybyreducingoxidativestressandhmgb1release AT madaqing protectiveeffectsofhydrogenenrichedsalineonliverischemiareperfusioninjurybyreducingoxidativestressandhmgb1release AT yuweifeng protectiveeffectsofhydrogenenrichedsalineonliverischemiareperfusioninjurybyreducingoxidativestressandhmgb1release |