The effect of delay in collection and processing on RNA integrity in human placenta: Experiences from rural Africa()

This paper examines the relationship between time to processing and RNA quality in placentas collected from women in a field setting in rural Gambia. Placental samples were collected from the villages and transferred to the laboratory. RNA was extracted using Trizol and integrity assessed using the...

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Detalles Bibliográficos
Autores principales: Jobarteh, M.L., Moore, S.E., Kennedy, C., Gambling, L., McArdle, H.J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2014
Materias:
Technical Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3928992/
https://www.ncbi.nlm.nih.gov/pubmed/24125806
http://dx.doi.org/10.1016/j.placenta.2013.08.016
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author Jobarteh, M.L.
Moore, S.E.
Kennedy, C.
Gambling, L.
McArdle, H.J.
author_facet Jobarteh, M.L.
Moore, S.E.
Kennedy, C.
Gambling, L.
McArdle, H.J.
author_sort Jobarteh, M.L.
collection PubMed
description This paper examines the relationship between time to processing and RNA quality in placentas collected from women in a field setting in rural Gambia. Placental samples were collected from the villages and transferred to the laboratory. RNA was extracted using Trizol and integrity assessed using the RNA integrity number (RIN). Values were inversely correlated with delay in processing. Expression levels of candidate genes increased with decreasing RIN. Normalising to a housekeeper gene removed this artefact. We propose a cut-off point of 90 min from delivery, after which samples cannot be used for gene expression analysis.
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spelling pubmed-39289922014-03-12 The effect of delay in collection and processing on RNA integrity in human placenta: Experiences from rural Africa() Jobarteh, M.L. Moore, S.E. Kennedy, C. Gambling, L. McArdle, H.J. Placenta Technical Note This paper examines the relationship between time to processing and RNA quality in placentas collected from women in a field setting in rural Gambia. Placental samples were collected from the villages and transferred to the laboratory. RNA was extracted using Trizol and integrity assessed using the RNA integrity number (RIN). Values were inversely correlated with delay in processing. Expression levels of candidate genes increased with decreasing RIN. Normalising to a housekeeper gene removed this artefact. We propose a cut-off point of 90 min from delivery, after which samples cannot be used for gene expression analysis. Elsevier 2014-01 /pmc/articles/PMC3928992/ /pubmed/24125806 http://dx.doi.org/10.1016/j.placenta.2013.08.016 Text en © 2013 The Authors https://creativecommons.org/licenses/by-nc-nd/3.0/This is an open access article under the CC BY NC ND license (https://creativecommons.org/licenses/by-nc-nd/3.0/).
spellingShingle Technical Note
Jobarteh, M.L.
Moore, S.E.
Kennedy, C.
Gambling, L.
McArdle, H.J.
The effect of delay in collection and processing on RNA integrity in human placenta: Experiences from rural Africa()
title The effect of delay in collection and processing on RNA integrity in human placenta: Experiences from rural Africa()
title_full The effect of delay in collection and processing on RNA integrity in human placenta: Experiences from rural Africa()
title_fullStr The effect of delay in collection and processing on RNA integrity in human placenta: Experiences from rural Africa()
title_full_unstemmed The effect of delay in collection and processing on RNA integrity in human placenta: Experiences from rural Africa()
title_short The effect of delay in collection and processing on RNA integrity in human placenta: Experiences from rural Africa()
title_sort effect of delay in collection and processing on rna integrity in human placenta: experiences from rural africa()
topic Technical Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3928992/
https://www.ncbi.nlm.nih.gov/pubmed/24125806
http://dx.doi.org/10.1016/j.placenta.2013.08.016
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