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Potent Anti-Inflammatory and Antiproliferative Effects of Gambogic Acid in a Rat Model of Antigen-Induced Arthritis

Background. We have previously reported a continuous activation of caspase-1 and increased interleukin (IL)-1β levels in early rheumatoid arthritis (RA). These observations raised the hypothesis that drugs targeting the IL-1β pathway, in addition to tumour necrosis factor (TNF), may be particularly...

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Autores principales: Cascão, Rita, Vidal, Bruno, Raquel, Helena, Neves-Costa, Ana, Figueiredo, Nuno, Gupta, Vineet, Fonseca, João Eurico, Ferreira Moita, Luis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3929289/
https://www.ncbi.nlm.nih.gov/pubmed/24623960
http://dx.doi.org/10.1155/2014/195327
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author Cascão, Rita
Vidal, Bruno
Raquel, Helena
Neves-Costa, Ana
Figueiredo, Nuno
Gupta, Vineet
Fonseca, João Eurico
Ferreira Moita, Luis
author_facet Cascão, Rita
Vidal, Bruno
Raquel, Helena
Neves-Costa, Ana
Figueiredo, Nuno
Gupta, Vineet
Fonseca, João Eurico
Ferreira Moita, Luis
author_sort Cascão, Rita
collection PubMed
description Background. We have previously reported a continuous activation of caspase-1 and increased interleukin (IL)-1β levels in early rheumatoid arthritis (RA). These observations raised the hypothesis that drugs targeting the IL-1β pathway, in addition to tumour necrosis factor (TNF), may be particularly effective for early RA treatment. We have recently identified gambogic acid as a promising therapeutic candidate to simultaneously block IL-1β and TNF secretion. Our main goal here was to investigate whether gambogic acid administration was able to attenuate inflammation in antigen-induced arthritis (AIA) rats. Methods. Gambogic acid was administered to AIA rats in the early and late phases of arthritis. The inflammatory score, ankle perimeter, and body weight were evaluated during the period of treatment. Rats were sacrificed after 19 days of disease progression and paw samples were collected for histological and immunohistochemical evaluation. Results. We found that inflammation in joints was significantly suppressed following gambogic acid administration. Histological and immunohistochemical evaluation of treated rats revealed normal joint structures with complete abrogation of the inflammatory infiltrate and cellular proliferation. Conclusions. Our results suggest that gambogic acid has significant anti-inflammatory properties and can possibly constitute a prototype anti-inflammatory drug with therapeutic efficacy in the treatment of inflammatory diseases such as RA.
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spelling pubmed-39292892014-03-12 Potent Anti-Inflammatory and Antiproliferative Effects of Gambogic Acid in a Rat Model of Antigen-Induced Arthritis Cascão, Rita Vidal, Bruno Raquel, Helena Neves-Costa, Ana Figueiredo, Nuno Gupta, Vineet Fonseca, João Eurico Ferreira Moita, Luis Mediators Inflamm Research Article Background. We have previously reported a continuous activation of caspase-1 and increased interleukin (IL)-1β levels in early rheumatoid arthritis (RA). These observations raised the hypothesis that drugs targeting the IL-1β pathway, in addition to tumour necrosis factor (TNF), may be particularly effective for early RA treatment. We have recently identified gambogic acid as a promising therapeutic candidate to simultaneously block IL-1β and TNF secretion. Our main goal here was to investigate whether gambogic acid administration was able to attenuate inflammation in antigen-induced arthritis (AIA) rats. Methods. Gambogic acid was administered to AIA rats in the early and late phases of arthritis. The inflammatory score, ankle perimeter, and body weight were evaluated during the period of treatment. Rats were sacrificed after 19 days of disease progression and paw samples were collected for histological and immunohistochemical evaluation. Results. We found that inflammation in joints was significantly suppressed following gambogic acid administration. Histological and immunohistochemical evaluation of treated rats revealed normal joint structures with complete abrogation of the inflammatory infiltrate and cellular proliferation. Conclusions. Our results suggest that gambogic acid has significant anti-inflammatory properties and can possibly constitute a prototype anti-inflammatory drug with therapeutic efficacy in the treatment of inflammatory diseases such as RA. Hindawi Publishing Corporation 2014 2014-01-30 /pmc/articles/PMC3929289/ /pubmed/24623960 http://dx.doi.org/10.1155/2014/195327 Text en Copyright © 2014 Rita Cascão et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Cascão, Rita
Vidal, Bruno
Raquel, Helena
Neves-Costa, Ana
Figueiredo, Nuno
Gupta, Vineet
Fonseca, João Eurico
Ferreira Moita, Luis
Potent Anti-Inflammatory and Antiproliferative Effects of Gambogic Acid in a Rat Model of Antigen-Induced Arthritis
title Potent Anti-Inflammatory and Antiproliferative Effects of Gambogic Acid in a Rat Model of Antigen-Induced Arthritis
title_full Potent Anti-Inflammatory and Antiproliferative Effects of Gambogic Acid in a Rat Model of Antigen-Induced Arthritis
title_fullStr Potent Anti-Inflammatory and Antiproliferative Effects of Gambogic Acid in a Rat Model of Antigen-Induced Arthritis
title_full_unstemmed Potent Anti-Inflammatory and Antiproliferative Effects of Gambogic Acid in a Rat Model of Antigen-Induced Arthritis
title_short Potent Anti-Inflammatory and Antiproliferative Effects of Gambogic Acid in a Rat Model of Antigen-Induced Arthritis
title_sort potent anti-inflammatory and antiproliferative effects of gambogic acid in a rat model of antigen-induced arthritis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3929289/
https://www.ncbi.nlm.nih.gov/pubmed/24623960
http://dx.doi.org/10.1155/2014/195327
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