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Optimal Immobilization of β-Galactosidase onto κ-Carrageenan Gel Beads Using Response Surface Methodology and Its Applications
β-Galactosidase (β-gal) was immobilized by covalent binding on novel κ-carrageenan gel beads activated by two-step method; the gel beads were soaked in polyethyleneimine followed by glutaraldehyde. 2(2) full-factorial central composite experiment designs were employed to optimize the conditions for...
Autores principales: | Elnashar, Magdy M., Awad, Ghada E., Hassan, Mohamed E., Mohy Eldin, Mohamed S., Haroun, Bakry M., El-Diwany, Ahmed I. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2014
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3929382/ https://www.ncbi.nlm.nih.gov/pubmed/24672334 http://dx.doi.org/10.1155/2014/571682 |
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