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A DTC Niche Plexus Surrounds the Germline Stem Cell Pool in Caenorhabditis elegans
The mesenchymal distal tip cell (DTC) provides the niche for Caenorhabditis elegans germline stem cells (GSCs). The DTC has a complex cellular architecture: its cell body caps the distal gonadal end and contacts germ cells extensively, but it also includes multiple cellular processes that extend alo...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3929564/ https://www.ncbi.nlm.nih.gov/pubmed/24586318 http://dx.doi.org/10.1371/journal.pone.0088372 |
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author | Byrd, Dana T. Knobel, Karla Affeldt, Katharyn Crittenden, Sarah L. Kimble, Judith |
author_facet | Byrd, Dana T. Knobel, Karla Affeldt, Katharyn Crittenden, Sarah L. Kimble, Judith |
author_sort | Byrd, Dana T. |
collection | PubMed |
description | The mesenchymal distal tip cell (DTC) provides the niche for Caenorhabditis elegans germline stem cells (GSCs). The DTC has a complex cellular architecture: its cell body caps the distal gonadal end and contacts germ cells extensively, but it also includes multiple cellular processes that extend along the germline tube and intercalate between germ cells. Here we use the lag-2 DTC promoter to drive expression of myristoylated GFP, which highlights DTC membranes and permits a more detailed view of DTC architecture. We find that short processes intercalating between germ cells contact more germ cells than seen previously. We define this region of extensive niche contact with germ cells as the DTC plexus. The extent of the DTC plexus corresponds well with the previously determined extent of the GSC pool. Moreover, expression of a differentiation marker increases as germ cells move out of the plexus. Maintenance of this DTC plexus depends on the presence of undifferentiated germ cells, suggesting that germ cell state can influence niche architecture. The roles of this DTC architecture remain an open question. One idea is that the DTC plexus delivers Notch signaling to the cluster of germ cells comprising the GSC pool; another idea is that the plexus anchors GSCs at the distal end. |
format | Online Article Text |
id | pubmed-3929564 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-39295642014-02-25 A DTC Niche Plexus Surrounds the Germline Stem Cell Pool in Caenorhabditis elegans Byrd, Dana T. Knobel, Karla Affeldt, Katharyn Crittenden, Sarah L. Kimble, Judith PLoS One Research Article The mesenchymal distal tip cell (DTC) provides the niche for Caenorhabditis elegans germline stem cells (GSCs). The DTC has a complex cellular architecture: its cell body caps the distal gonadal end and contacts germ cells extensively, but it also includes multiple cellular processes that extend along the germline tube and intercalate between germ cells. Here we use the lag-2 DTC promoter to drive expression of myristoylated GFP, which highlights DTC membranes and permits a more detailed view of DTC architecture. We find that short processes intercalating between germ cells contact more germ cells than seen previously. We define this region of extensive niche contact with germ cells as the DTC plexus. The extent of the DTC plexus corresponds well with the previously determined extent of the GSC pool. Moreover, expression of a differentiation marker increases as germ cells move out of the plexus. Maintenance of this DTC plexus depends on the presence of undifferentiated germ cells, suggesting that germ cell state can influence niche architecture. The roles of this DTC architecture remain an open question. One idea is that the DTC plexus delivers Notch signaling to the cluster of germ cells comprising the GSC pool; another idea is that the plexus anchors GSCs at the distal end. Public Library of Science 2014-02-19 /pmc/articles/PMC3929564/ /pubmed/24586318 http://dx.doi.org/10.1371/journal.pone.0088372 Text en © 2014 Byrd et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Byrd, Dana T. Knobel, Karla Affeldt, Katharyn Crittenden, Sarah L. Kimble, Judith A DTC Niche Plexus Surrounds the Germline Stem Cell Pool in Caenorhabditis elegans |
title | A DTC Niche Plexus Surrounds the Germline Stem Cell Pool in Caenorhabditis elegans
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title_full | A DTC Niche Plexus Surrounds the Germline Stem Cell Pool in Caenorhabditis elegans
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title_fullStr | A DTC Niche Plexus Surrounds the Germline Stem Cell Pool in Caenorhabditis elegans
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title_full_unstemmed | A DTC Niche Plexus Surrounds the Germline Stem Cell Pool in Caenorhabditis elegans
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title_short | A DTC Niche Plexus Surrounds the Germline Stem Cell Pool in Caenorhabditis elegans
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title_sort | dtc niche plexus surrounds the germline stem cell pool in caenorhabditis elegans |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3929564/ https://www.ncbi.nlm.nih.gov/pubmed/24586318 http://dx.doi.org/10.1371/journal.pone.0088372 |
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