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Imaging Dynamic Molecular Signaling by the Cdc42 GTPase within the Developing CNS

Protein interactions underlie the complexity of neuronal function. Potential interactions between specific proteins in the brain are predicted from assays based on genetic interaction and/or biochemistry. Genetic interaction reveals endogenous, but not necessarily direct, interactions between the pr...

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Detalles Bibliográficos
Autores principales: Sharifai, Nima, Samarajeewa, Hasitha, Kamiyama, Daichi, Deng, Tzyy-Chyn, Boulina, Maria, Chiba, Akira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3929599/
https://www.ncbi.nlm.nih.gov/pubmed/24586421
http://dx.doi.org/10.1371/journal.pone.0088870
Descripción
Sumario:Protein interactions underlie the complexity of neuronal function. Potential interactions between specific proteins in the brain are predicted from assays based on genetic interaction and/or biochemistry. Genetic interaction reveals endogenous, but not necessarily direct, interactions between the proteins. Biochemistry-based assays, on the other hand, demonstrate direct interactions between proteins, but often outside their native environment or without a subcellular context. We aimed to achieve the best of both approaches by visualizing protein interaction directly within the brain of a live animal. Here, we show a proof-of-principle experiment in which the Cdc42 GTPase associates with its alleged partner WASp within neurons during the time and space that coincide with the newly developing CNS.