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Effects of Repeated Administration of Pilocarpine and Isoproterenol on Aquaporin-5 Expression in Rat Salivary Glands

Aquaporins are water channel proteins which enable rapid water movement across the plasma membrane. Aquaporin-5 (AQP5) is the major aquaporin and is expressed on the apical membrane of salivary gland acinar cells. We examined the effects of repeated administration of pilocarpine, a clinically useful...

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Autores principales: Susa, Taketo, Sawai, Nobuhiko, Aoki, Takeo, Iizuka-Kogo, Akiko, Kogo, Hiroshi, Negishi, Akihide, Yokoo, Satoshi, Takata, Kuniaki, Matsuzaki, Toshiyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japan Society of Histochemistry and Cytochemistry 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3929617/
https://www.ncbi.nlm.nih.gov/pubmed/24610966
http://dx.doi.org/10.1267/ahc.13037
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author Susa, Taketo
Sawai, Nobuhiko
Aoki, Takeo
Iizuka-Kogo, Akiko
Kogo, Hiroshi
Negishi, Akihide
Yokoo, Satoshi
Takata, Kuniaki
Matsuzaki, Toshiyuki
author_facet Susa, Taketo
Sawai, Nobuhiko
Aoki, Takeo
Iizuka-Kogo, Akiko
Kogo, Hiroshi
Negishi, Akihide
Yokoo, Satoshi
Takata, Kuniaki
Matsuzaki, Toshiyuki
author_sort Susa, Taketo
collection PubMed
description Aquaporins are water channel proteins which enable rapid water movement across the plasma membrane. Aquaporin-5 (AQP5) is the major aquaporin and is expressed on the apical membrane of salivary gland acinar cells. We examined the effects of repeated administration of pilocarpine, a clinically useful stimulant for salivary fluid secretion, and isoproterenol (IPR), a stimulant for salivary protein secretion, on the abundance of AQP5 protein in rat salivary glands by immunofluorescence microscopy and semi-quantitative immunoblotting. Unexpectedly AQP5 was decreased in pilocarpine-administered salivary glands, in which fluid secretion must be highly stimulated, implying that AQP5 might not be required for fluid secretion at least in pilocarpine-administered state. The abundance of AQP5, on the other hand, was found to be significantly increased in IPR-administered submandibular and parotid glands. To address the possible mechanism of the elevation of AQP5 abundance in IPR-administered animals, changes of AQP5 level in fasting animals, in which the exocytotic events are reduced, were examined. AQP5 was found to be decreased in fasting animals as expected. These results suggested that the elevation of cAMP and/or frequent exocytotic events could increase AQP5 protein. AQP5 expression seems to be easily changed by salivary stimulants, although these changes do not always reflect the ability in salivary fluid secretion.
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spelling pubmed-39296172014-04-01 Effects of Repeated Administration of Pilocarpine and Isoproterenol on Aquaporin-5 Expression in Rat Salivary Glands Susa, Taketo Sawai, Nobuhiko Aoki, Takeo Iizuka-Kogo, Akiko Kogo, Hiroshi Negishi, Akihide Yokoo, Satoshi Takata, Kuniaki Matsuzaki, Toshiyuki Acta Histochem Cytochem Regular Article Aquaporins are water channel proteins which enable rapid water movement across the plasma membrane. Aquaporin-5 (AQP5) is the major aquaporin and is expressed on the apical membrane of salivary gland acinar cells. We examined the effects of repeated administration of pilocarpine, a clinically useful stimulant for salivary fluid secretion, and isoproterenol (IPR), a stimulant for salivary protein secretion, on the abundance of AQP5 protein in rat salivary glands by immunofluorescence microscopy and semi-quantitative immunoblotting. Unexpectedly AQP5 was decreased in pilocarpine-administered salivary glands, in which fluid secretion must be highly stimulated, implying that AQP5 might not be required for fluid secretion at least in pilocarpine-administered state. The abundance of AQP5, on the other hand, was found to be significantly increased in IPR-administered submandibular and parotid glands. To address the possible mechanism of the elevation of AQP5 abundance in IPR-administered animals, changes of AQP5 level in fasting animals, in which the exocytotic events are reduced, were examined. AQP5 was found to be decreased in fasting animals as expected. These results suggested that the elevation of cAMP and/or frequent exocytotic events could increase AQP5 protein. AQP5 expression seems to be easily changed by salivary stimulants, although these changes do not always reflect the ability in salivary fluid secretion. Japan Society of Histochemistry and Cytochemistry 2013-12-28 2013-12-25 /pmc/articles/PMC3929617/ /pubmed/24610966 http://dx.doi.org/10.1267/ahc.13037 Text en © 2013 The Japan Society of Histochemistry and Cytochemistry This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Regular Article
Susa, Taketo
Sawai, Nobuhiko
Aoki, Takeo
Iizuka-Kogo, Akiko
Kogo, Hiroshi
Negishi, Akihide
Yokoo, Satoshi
Takata, Kuniaki
Matsuzaki, Toshiyuki
Effects of Repeated Administration of Pilocarpine and Isoproterenol on Aquaporin-5 Expression in Rat Salivary Glands
title Effects of Repeated Administration of Pilocarpine and Isoproterenol on Aquaporin-5 Expression in Rat Salivary Glands
title_full Effects of Repeated Administration of Pilocarpine and Isoproterenol on Aquaporin-5 Expression in Rat Salivary Glands
title_fullStr Effects of Repeated Administration of Pilocarpine and Isoproterenol on Aquaporin-5 Expression in Rat Salivary Glands
title_full_unstemmed Effects of Repeated Administration of Pilocarpine and Isoproterenol on Aquaporin-5 Expression in Rat Salivary Glands
title_short Effects of Repeated Administration of Pilocarpine and Isoproterenol on Aquaporin-5 Expression in Rat Salivary Glands
title_sort effects of repeated administration of pilocarpine and isoproterenol on aquaporin-5 expression in rat salivary glands
topic Regular Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3929617/
https://www.ncbi.nlm.nih.gov/pubmed/24610966
http://dx.doi.org/10.1267/ahc.13037
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