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Measuring Cytotoxicity by Bioluminescence Imaging Outperforms the Standard Chromium-51 Release Assay
The chromium-release assay developed in 1968 is still the most commonly used method to measure cytotoxicity by T cells and by natural killer cells. Target cells are loaded in vitro with radioactive chromium and lysis is determined by measuring chromium in the supernatant released by dying cells. Sin...
Autores principales: | Karimi, Mobin A., Lee, Eric, Bachmann, Michael H., Salicioni, Ana Maria, Behrens, Edward M., Kambayashi, Taku, Baldwin, Cynthia L. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3929704/ https://www.ncbi.nlm.nih.gov/pubmed/24586714 http://dx.doi.org/10.1371/journal.pone.0089357 |
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