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Growth/differentiation factor 15 promotes EGFR signalling, and regulates proliferation and migration in the hippocampus of neonatal and young adult mice

The activation of epidermal growth factor receptor (EGFR) affects multiple aspects of neural precursor behaviour, including proliferation and migration. Telencephalic precursors acquire EGF responsiveness and upregulate EGFR expression at late stages of development. The events regulating this proces...

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Autores principales: Carrillo-García, Carmen, Prochnow, Sebastian, Simeonova, Ina K., Strelau, Jens, Hölzl-Wenig, Gabriele, Mandl, Claudia, Unsicker, Klaus, von Bohlen und Halbach, Oliver, Ciccolini, Francesca
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Company of Biologists 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3930467/
https://www.ncbi.nlm.nih.gov/pubmed/24496615
http://dx.doi.org/10.1242/dev.096131
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author Carrillo-García, Carmen
Prochnow, Sebastian
Simeonova, Ina K.
Strelau, Jens
Hölzl-Wenig, Gabriele
Mandl, Claudia
Unsicker, Klaus
von Bohlen und Halbach, Oliver
Ciccolini, Francesca
author_facet Carrillo-García, Carmen
Prochnow, Sebastian
Simeonova, Ina K.
Strelau, Jens
Hölzl-Wenig, Gabriele
Mandl, Claudia
Unsicker, Klaus
von Bohlen und Halbach, Oliver
Ciccolini, Francesca
author_sort Carrillo-García, Carmen
collection PubMed
description The activation of epidermal growth factor receptor (EGFR) affects multiple aspects of neural precursor behaviour, including proliferation and migration. Telencephalic precursors acquire EGF responsiveness and upregulate EGFR expression at late stages of development. The events regulating this process and its significance are still unclear. We here show that in the developing and postnatal hippocampus (HP), growth/differentiation factor (GDF) 15 and EGFR are co-expressed in primitive precursors as well as in more differentiated cells. We also provide evidence that GDF15 promotes responsiveness to EGF and EGFR expression in hippocampal precursors through a mechanism that requires active CXC chemokine receptor (CXCR) 4. Besides EGFR expression, GDF15 ablation also leads to decreased proliferation and migration. In particular, lack of GDF15 impairs both processes in the cornu ammonis (CA) 1 and only proliferation in the dentate gyrus (DG). Importantly, migration and proliferation in the mutant HP were altered only perinatally, when EGFR expression was also affected. These data suggest that GDF15 regulates migration and proliferation by promoting EGFR signalling in the perinatal HP and represent a first description of a functional role for GDF15 in the developing telencephalon.
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spelling pubmed-39304672014-02-25 Growth/differentiation factor 15 promotes EGFR signalling, and regulates proliferation and migration in the hippocampus of neonatal and young adult mice Carrillo-García, Carmen Prochnow, Sebastian Simeonova, Ina K. Strelau, Jens Hölzl-Wenig, Gabriele Mandl, Claudia Unsicker, Klaus von Bohlen und Halbach, Oliver Ciccolini, Francesca Development Stem Cells and Regeneration The activation of epidermal growth factor receptor (EGFR) affects multiple aspects of neural precursor behaviour, including proliferation and migration. Telencephalic precursors acquire EGF responsiveness and upregulate EGFR expression at late stages of development. The events regulating this process and its significance are still unclear. We here show that in the developing and postnatal hippocampus (HP), growth/differentiation factor (GDF) 15 and EGFR are co-expressed in primitive precursors as well as in more differentiated cells. We also provide evidence that GDF15 promotes responsiveness to EGF and EGFR expression in hippocampal precursors through a mechanism that requires active CXC chemokine receptor (CXCR) 4. Besides EGFR expression, GDF15 ablation also leads to decreased proliferation and migration. In particular, lack of GDF15 impairs both processes in the cornu ammonis (CA) 1 and only proliferation in the dentate gyrus (DG). Importantly, migration and proliferation in the mutant HP were altered only perinatally, when EGFR expression was also affected. These data suggest that GDF15 regulates migration and proliferation by promoting EGFR signalling in the perinatal HP and represent a first description of a functional role for GDF15 in the developing telencephalon. Company of Biologists 2014-02-15 /pmc/articles/PMC3930467/ /pubmed/24496615 http://dx.doi.org/10.1242/dev.096131 Text en © 2014. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by-nc-sa/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
spellingShingle Stem Cells and Regeneration
Carrillo-García, Carmen
Prochnow, Sebastian
Simeonova, Ina K.
Strelau, Jens
Hölzl-Wenig, Gabriele
Mandl, Claudia
Unsicker, Klaus
von Bohlen und Halbach, Oliver
Ciccolini, Francesca
Growth/differentiation factor 15 promotes EGFR signalling, and regulates proliferation and migration in the hippocampus of neonatal and young adult mice
title Growth/differentiation factor 15 promotes EGFR signalling, and regulates proliferation and migration in the hippocampus of neonatal and young adult mice
title_full Growth/differentiation factor 15 promotes EGFR signalling, and regulates proliferation and migration in the hippocampus of neonatal and young adult mice
title_fullStr Growth/differentiation factor 15 promotes EGFR signalling, and regulates proliferation and migration in the hippocampus of neonatal and young adult mice
title_full_unstemmed Growth/differentiation factor 15 promotes EGFR signalling, and regulates proliferation and migration in the hippocampus of neonatal and young adult mice
title_short Growth/differentiation factor 15 promotes EGFR signalling, and regulates proliferation and migration in the hippocampus of neonatal and young adult mice
title_sort growth/differentiation factor 15 promotes egfr signalling, and regulates proliferation and migration in the hippocampus of neonatal and young adult mice
topic Stem Cells and Regeneration
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3930467/
https://www.ncbi.nlm.nih.gov/pubmed/24496615
http://dx.doi.org/10.1242/dev.096131
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