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A Compositional Look at the Human Gastrointestinal Microbiome and Immune Activation Parameters in HIV Infected Subjects
HIV progression is characterized by immune activation and microbial translocation. One factor that may be contributing to HIV progression could be a dysbiotic microbiome. We therefore hypothesized that the GI mucosal microbiome is altered in HIV patients and this alteration correlates with immune ac...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3930561/ https://www.ncbi.nlm.nih.gov/pubmed/24586144 http://dx.doi.org/10.1371/journal.ppat.1003829 |
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author | Mutlu, Ece A. Keshavarzian, Ali Losurdo, John Swanson, Garth Siewe, Basile Forsyth, Christopher French, Audrey DeMarais, Patricia Sun, Yan Koenig, Lars Cox, Stephen Engen, Phillip Chakradeo, Prachi Abbasi, Rawan Gorenz, Annika Burns, Charles Landay, Alan |
author_facet | Mutlu, Ece A. Keshavarzian, Ali Losurdo, John Swanson, Garth Siewe, Basile Forsyth, Christopher French, Audrey DeMarais, Patricia Sun, Yan Koenig, Lars Cox, Stephen Engen, Phillip Chakradeo, Prachi Abbasi, Rawan Gorenz, Annika Burns, Charles Landay, Alan |
author_sort | Mutlu, Ece A. |
collection | PubMed |
description | HIV progression is characterized by immune activation and microbial translocation. One factor that may be contributing to HIV progression could be a dysbiotic microbiome. We therefore hypothesized that the GI mucosal microbiome is altered in HIV patients and this alteration correlates with immune activation in HIV. 121 specimens were collected from 21 HIV positive and 22 control human subjects during colonoscopy. The composition of the lower gastrointestinal tract mucosal and luminal bacterial microbiome was characterized using 16S rDNA pyrosequencing and was correlated to clinical parameters as well as immune activation and circulating bacterial products in HIV patients on ART. The composition of the HIV microbiome was significantly different than that of controls; it was less diverse in the right colon and terminal ileum, and was characterized by loss of bacterial taxa that are typically considered commensals. In HIV samples, there was a gain of some pathogenic bacterial taxa. This is the first report characterizing the terminal ileal and colonic mucosal microbiome in HIV patients with next generation sequencing. Limitations include use of HIV-infected subjects on HAART therapy. |
format | Online Article Text |
id | pubmed-3930561 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-39305612014-02-25 A Compositional Look at the Human Gastrointestinal Microbiome and Immune Activation Parameters in HIV Infected Subjects Mutlu, Ece A. Keshavarzian, Ali Losurdo, John Swanson, Garth Siewe, Basile Forsyth, Christopher French, Audrey DeMarais, Patricia Sun, Yan Koenig, Lars Cox, Stephen Engen, Phillip Chakradeo, Prachi Abbasi, Rawan Gorenz, Annika Burns, Charles Landay, Alan PLoS Pathog Research Article HIV progression is characterized by immune activation and microbial translocation. One factor that may be contributing to HIV progression could be a dysbiotic microbiome. We therefore hypothesized that the GI mucosal microbiome is altered in HIV patients and this alteration correlates with immune activation in HIV. 121 specimens were collected from 21 HIV positive and 22 control human subjects during colonoscopy. The composition of the lower gastrointestinal tract mucosal and luminal bacterial microbiome was characterized using 16S rDNA pyrosequencing and was correlated to clinical parameters as well as immune activation and circulating bacterial products in HIV patients on ART. The composition of the HIV microbiome was significantly different than that of controls; it was less diverse in the right colon and terminal ileum, and was characterized by loss of bacterial taxa that are typically considered commensals. In HIV samples, there was a gain of some pathogenic bacterial taxa. This is the first report characterizing the terminal ileal and colonic mucosal microbiome in HIV patients with next generation sequencing. Limitations include use of HIV-infected subjects on HAART therapy. Public Library of Science 2014-02-20 /pmc/articles/PMC3930561/ /pubmed/24586144 http://dx.doi.org/10.1371/journal.ppat.1003829 Text en © 2014 Mutlu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Mutlu, Ece A. Keshavarzian, Ali Losurdo, John Swanson, Garth Siewe, Basile Forsyth, Christopher French, Audrey DeMarais, Patricia Sun, Yan Koenig, Lars Cox, Stephen Engen, Phillip Chakradeo, Prachi Abbasi, Rawan Gorenz, Annika Burns, Charles Landay, Alan A Compositional Look at the Human Gastrointestinal Microbiome and Immune Activation Parameters in HIV Infected Subjects |
title | A Compositional Look at the Human Gastrointestinal Microbiome and Immune Activation Parameters in HIV Infected Subjects |
title_full | A Compositional Look at the Human Gastrointestinal Microbiome and Immune Activation Parameters in HIV Infected Subjects |
title_fullStr | A Compositional Look at the Human Gastrointestinal Microbiome and Immune Activation Parameters in HIV Infected Subjects |
title_full_unstemmed | A Compositional Look at the Human Gastrointestinal Microbiome and Immune Activation Parameters in HIV Infected Subjects |
title_short | A Compositional Look at the Human Gastrointestinal Microbiome and Immune Activation Parameters in HIV Infected Subjects |
title_sort | compositional look at the human gastrointestinal microbiome and immune activation parameters in hiv infected subjects |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3930561/ https://www.ncbi.nlm.nih.gov/pubmed/24586144 http://dx.doi.org/10.1371/journal.ppat.1003829 |
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