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Importin 7 and Nup358 Promote Nuclear Import of the Protein Component of Human Telomerase
In actively dividing eukaryotic cells, chromosome ends (telomeres) are subject to progressive shortening, unless they are maintained by the action of telomerase, a dedicated enzyme that adds DNA sequence repeats to chromosomal 3′end. For its enzymatic function on telomeres, telomerase requires nucle...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3930611/ https://www.ncbi.nlm.nih.gov/pubmed/24586428 http://dx.doi.org/10.1371/journal.pone.0088887 |
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author | Frohnert, Cornelia Hutten, Saskia Wälde, Sarah Nath, Annegret Kehlenbach, Ralph H. |
author_facet | Frohnert, Cornelia Hutten, Saskia Wälde, Sarah Nath, Annegret Kehlenbach, Ralph H. |
author_sort | Frohnert, Cornelia |
collection | PubMed |
description | In actively dividing eukaryotic cells, chromosome ends (telomeres) are subject to progressive shortening, unless they are maintained by the action of telomerase, a dedicated enzyme that adds DNA sequence repeats to chromosomal 3′end. For its enzymatic function on telomeres, telomerase requires nuclear import of its protein component (hTERT in human cells) and assembly with the RNA component, TERC. We now confirm a major nuclear localization signal (NLS) in the N-terminal region of hTERT and describe a novel one in the C-terminal part. Using an siRNA approach to deplete several import receptors, we identify importin 7 as a soluble nuclear transport factor that is required for efficient import. At the level of the nuclear pore complex (NPC), Nup358, a nucleoporin that forms the cytoplasmic filaments of the NPC, plays an important role in nuclear import of hTERT. A structure-function analysis of Nup358 revealed that the zinc finger region of the nucleoporin is of particular importance for transport of hTERT. Together, our study sheds light on the nuclear import pathway of hTERT. |
format | Online Article Text |
id | pubmed-3930611 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-39306112014-02-25 Importin 7 and Nup358 Promote Nuclear Import of the Protein Component of Human Telomerase Frohnert, Cornelia Hutten, Saskia Wälde, Sarah Nath, Annegret Kehlenbach, Ralph H. PLoS One Research Article In actively dividing eukaryotic cells, chromosome ends (telomeres) are subject to progressive shortening, unless they are maintained by the action of telomerase, a dedicated enzyme that adds DNA sequence repeats to chromosomal 3′end. For its enzymatic function on telomeres, telomerase requires nuclear import of its protein component (hTERT in human cells) and assembly with the RNA component, TERC. We now confirm a major nuclear localization signal (NLS) in the N-terminal region of hTERT and describe a novel one in the C-terminal part. Using an siRNA approach to deplete several import receptors, we identify importin 7 as a soluble nuclear transport factor that is required for efficient import. At the level of the nuclear pore complex (NPC), Nup358, a nucleoporin that forms the cytoplasmic filaments of the NPC, plays an important role in nuclear import of hTERT. A structure-function analysis of Nup358 revealed that the zinc finger region of the nucleoporin is of particular importance for transport of hTERT. Together, our study sheds light on the nuclear import pathway of hTERT. Public Library of Science 2014-02-20 /pmc/articles/PMC3930611/ /pubmed/24586428 http://dx.doi.org/10.1371/journal.pone.0088887 Text en © 2014 Frohnert et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Frohnert, Cornelia Hutten, Saskia Wälde, Sarah Nath, Annegret Kehlenbach, Ralph H. Importin 7 and Nup358 Promote Nuclear Import of the Protein Component of Human Telomerase |
title | Importin 7 and Nup358 Promote Nuclear Import of the Protein Component of Human Telomerase |
title_full | Importin 7 and Nup358 Promote Nuclear Import of the Protein Component of Human Telomerase |
title_fullStr | Importin 7 and Nup358 Promote Nuclear Import of the Protein Component of Human Telomerase |
title_full_unstemmed | Importin 7 and Nup358 Promote Nuclear Import of the Protein Component of Human Telomerase |
title_short | Importin 7 and Nup358 Promote Nuclear Import of the Protein Component of Human Telomerase |
title_sort | importin 7 and nup358 promote nuclear import of the protein component of human telomerase |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3930611/ https://www.ncbi.nlm.nih.gov/pubmed/24586428 http://dx.doi.org/10.1371/journal.pone.0088887 |
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