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CTRP2 Overexpression Improves Insulin and Lipid Tolerance in Diet-Induced Obese Mice
CTRP2 is a secreted plasma protein of the C1q family that enhances glycogen deposition and fat oxidation in cultured myotubes. Its in vivo metabolic function, however, has not been established. We show here that acute and chronic metabolic perturbations induced by fasting or high-fat feeding up-regu...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3930646/ https://www.ncbi.nlm.nih.gov/pubmed/24586339 http://dx.doi.org/10.1371/journal.pone.0088535 |
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author | Peterson, Jonathan M. Seldin, Marcus M. Tan, Stefanie Y. Wong, G. William |
author_facet | Peterson, Jonathan M. Seldin, Marcus M. Tan, Stefanie Y. Wong, G. William |
author_sort | Peterson, Jonathan M. |
collection | PubMed |
description | CTRP2 is a secreted plasma protein of the C1q family that enhances glycogen deposition and fat oxidation in cultured myotubes. Its in vivo metabolic function, however, has not been established. We show here that acute and chronic metabolic perturbations induced by fasting or high-fat feeding up-regulated the mRNA expression of Ctrp2 in white adipose tissue without affecting its circulating plasma levels. We generated a transgenic mouse model with elevated circulating levels of CTRP2 to determine its metabolic function in vivo. When fed a low-fat diet, wild-type and CTRP2 transgenic mice exhibited no metabolic phenotypes. When challenged with a high-fat diet to induce obesity, wild-type and CTRP2 transgenic mice had similar weight gain, adiposity, food intake, metabolic rate, and energy expenditure. Fasting serum lipid and adipokine profiles were also similar between the two groups of mice. However, while glucose and insulin levels in the fasted state were comparable between wild-type and CTRP2 transgenic mice, insulin levels in the fed state were consistently lower in transgenic mice. Notably, CTRP2 transgenic mice had improved insulin tolerance and a greater capacity to handle acute lipid challenge relative to littermate controls. Our results highlight, for the first time, the in vivo role of CTRP2 in modulating whole-body metabolism. |
format | Online Article Text |
id | pubmed-3930646 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-39306462014-02-25 CTRP2 Overexpression Improves Insulin and Lipid Tolerance in Diet-Induced Obese Mice Peterson, Jonathan M. Seldin, Marcus M. Tan, Stefanie Y. Wong, G. William PLoS One Research Article CTRP2 is a secreted plasma protein of the C1q family that enhances glycogen deposition and fat oxidation in cultured myotubes. Its in vivo metabolic function, however, has not been established. We show here that acute and chronic metabolic perturbations induced by fasting or high-fat feeding up-regulated the mRNA expression of Ctrp2 in white adipose tissue without affecting its circulating plasma levels. We generated a transgenic mouse model with elevated circulating levels of CTRP2 to determine its metabolic function in vivo. When fed a low-fat diet, wild-type and CTRP2 transgenic mice exhibited no metabolic phenotypes. When challenged with a high-fat diet to induce obesity, wild-type and CTRP2 transgenic mice had similar weight gain, adiposity, food intake, metabolic rate, and energy expenditure. Fasting serum lipid and adipokine profiles were also similar between the two groups of mice. However, while glucose and insulin levels in the fasted state were comparable between wild-type and CTRP2 transgenic mice, insulin levels in the fed state were consistently lower in transgenic mice. Notably, CTRP2 transgenic mice had improved insulin tolerance and a greater capacity to handle acute lipid challenge relative to littermate controls. Our results highlight, for the first time, the in vivo role of CTRP2 in modulating whole-body metabolism. Public Library of Science 2014-02-20 /pmc/articles/PMC3930646/ /pubmed/24586339 http://dx.doi.org/10.1371/journal.pone.0088535 Text en © 2014 Peterson et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Peterson, Jonathan M. Seldin, Marcus M. Tan, Stefanie Y. Wong, G. William CTRP2 Overexpression Improves Insulin and Lipid Tolerance in Diet-Induced Obese Mice |
title | CTRP2 Overexpression Improves Insulin and Lipid Tolerance in Diet-Induced Obese Mice |
title_full | CTRP2 Overexpression Improves Insulin and Lipid Tolerance in Diet-Induced Obese Mice |
title_fullStr | CTRP2 Overexpression Improves Insulin and Lipid Tolerance in Diet-Induced Obese Mice |
title_full_unstemmed | CTRP2 Overexpression Improves Insulin and Lipid Tolerance in Diet-Induced Obese Mice |
title_short | CTRP2 Overexpression Improves Insulin and Lipid Tolerance in Diet-Induced Obese Mice |
title_sort | ctrp2 overexpression improves insulin and lipid tolerance in diet-induced obese mice |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3930646/ https://www.ncbi.nlm.nih.gov/pubmed/24586339 http://dx.doi.org/10.1371/journal.pone.0088535 |
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