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Relationship of CK8/18 expression pattern to breast cancer immunohistochemical subtyping in Egyptian patients
The immunohistochemical (IHC) subtyping of breast cancer can be a useful substitute for gene expression analysis. The aim of this study was to investigate the relationship of CK8/18 to the biology of breast carcinoma (BC) represented by its IHC subtypes. The IHC expression of CK8/18 was correlated w...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cancer Intelligence
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3931531/ https://www.ncbi.nlm.nih.gov/pubmed/24605136 http://dx.doi.org/10.3332/ecancer.2014.404 |
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author | Aiad, Hayam A Samaka, Rehab M Asaad, Nancy Y Kandil, Mona A Shehata, Mohamed A Miligy, Islam M |
author_facet | Aiad, Hayam A Samaka, Rehab M Asaad, Nancy Y Kandil, Mona A Shehata, Mohamed A Miligy, Islam M |
author_sort | Aiad, Hayam A |
collection | PubMed |
description | The immunohistochemical (IHC) subtyping of breast cancer can be a useful substitute for gene expression analysis. The aim of this study was to investigate the relationship of CK8/18 to the biology of breast carcinoma (BC) represented by its IHC subtypes. The IHC expression of CK8/18 was correlated with IHC subtypes of BC using ER, PR, HER2/neu, and Ki67 LI (with cutoff 14%). All cases showed CK 8/18 expression in tumour cells with varying degree of intensities; 49/70 cases (70%) showed diffuse cytoplasmic expression (loss of membranous pattern), while 21/70 cases (30%) showed membrano-cytoplasmic pattern. Adjacent non-neoplastic breast lobules showed membrano-cytoplasmic pattern in 58% of cases, which was significantly different from the pattern in invasive cancer (P = 0.002). A loss of membranous pattern in malignant tumours was significantly associated with higher tumour grade (P = 0.02), higher mitotic count (P = 0.03), and negative HER2/neu status (P = 0.04). CK 8/18 H score ranged between 1 and 290 with mean ± SD was 181 ± 70.54. Tumours with lower CK 8/18 H score were in the advanced stage group (P = 0.04). Low CK8/18 H score and loss of membranous pattern were significantly associated with triple negative (TN) subtype as compared with luminal subtype (P = 0.006 and P = 0.026, respectively). In addition, CK8/18 with lost membranous pattern was significantly associated with TN subtype compared with HER2/neu positive subtype (P = 0.001). However, there was no significant difference between luminal A and B subtypes regarding CK8/18 H score or pattern of expression. This study concluded that low CK8/18 H score and loss of membranous pattern of CK8/18 are associated with worse prognostic features and TN subtype. |
format | Online Article Text |
id | pubmed-3931531 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Cancer Intelligence |
record_format | MEDLINE/PubMed |
spelling | pubmed-39315312014-03-06 Relationship of CK8/18 expression pattern to breast cancer immunohistochemical subtyping in Egyptian patients Aiad, Hayam A Samaka, Rehab M Asaad, Nancy Y Kandil, Mona A Shehata, Mohamed A Miligy, Islam M Ecancermedicalscience Research The immunohistochemical (IHC) subtyping of breast cancer can be a useful substitute for gene expression analysis. The aim of this study was to investigate the relationship of CK8/18 to the biology of breast carcinoma (BC) represented by its IHC subtypes. The IHC expression of CK8/18 was correlated with IHC subtypes of BC using ER, PR, HER2/neu, and Ki67 LI (with cutoff 14%). All cases showed CK 8/18 expression in tumour cells with varying degree of intensities; 49/70 cases (70%) showed diffuse cytoplasmic expression (loss of membranous pattern), while 21/70 cases (30%) showed membrano-cytoplasmic pattern. Adjacent non-neoplastic breast lobules showed membrano-cytoplasmic pattern in 58% of cases, which was significantly different from the pattern in invasive cancer (P = 0.002). A loss of membranous pattern in malignant tumours was significantly associated with higher tumour grade (P = 0.02), higher mitotic count (P = 0.03), and negative HER2/neu status (P = 0.04). CK 8/18 H score ranged between 1 and 290 with mean ± SD was 181 ± 70.54. Tumours with lower CK 8/18 H score were in the advanced stage group (P = 0.04). Low CK8/18 H score and loss of membranous pattern were significantly associated with triple negative (TN) subtype as compared with luminal subtype (P = 0.006 and P = 0.026, respectively). In addition, CK8/18 with lost membranous pattern was significantly associated with TN subtype compared with HER2/neu positive subtype (P = 0.001). However, there was no significant difference between luminal A and B subtypes regarding CK8/18 H score or pattern of expression. This study concluded that low CK8/18 H score and loss of membranous pattern of CK8/18 are associated with worse prognostic features and TN subtype. Cancer Intelligence 2014-02-20 /pmc/articles/PMC3931531/ /pubmed/24605136 http://dx.doi.org/10.3332/ecancer.2014.404 Text en © the authors; licensee ecancermedicalscience. http://creativecommons.org/licenses/by/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Aiad, Hayam A Samaka, Rehab M Asaad, Nancy Y Kandil, Mona A Shehata, Mohamed A Miligy, Islam M Relationship of CK8/18 expression pattern to breast cancer immunohistochemical subtyping in Egyptian patients |
title | Relationship of CK8/18 expression pattern to breast cancer immunohistochemical subtyping in Egyptian patients |
title_full | Relationship of CK8/18 expression pattern to breast cancer immunohistochemical subtyping in Egyptian patients |
title_fullStr | Relationship of CK8/18 expression pattern to breast cancer immunohistochemical subtyping in Egyptian patients |
title_full_unstemmed | Relationship of CK8/18 expression pattern to breast cancer immunohistochemical subtyping in Egyptian patients |
title_short | Relationship of CK8/18 expression pattern to breast cancer immunohistochemical subtyping in Egyptian patients |
title_sort | relationship of ck8/18 expression pattern to breast cancer immunohistochemical subtyping in egyptian patients |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3931531/ https://www.ncbi.nlm.nih.gov/pubmed/24605136 http://dx.doi.org/10.3332/ecancer.2014.404 |
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