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Isolation and Molecular Characterization of a Novel Picornavirus from Baitfish in the USA

During both regulatory and routine surveillance sampling of baitfish from the states of Illinois, Minnesota, Montana, and Wisconsin, USA, isolates (n = 20) of a previously unknown picornavirus were obtained from kidney/spleen or entire viscera of fathead minnows (Pimephales promelas) and brassy minn...

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Autores principales: Phelps, Nicholas B. D., Mor, Sunil K., Armien, Anibal G., Batts, William, Goodwin, Andrew E., Hopper, Lacey, McCann, Rebekah, Ng, Terry Fei Fan, Puzach, Corey, Waltzek, Thomas B., Delwart, Eric, Winton, James, Goyal, Sagar M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3931614/
https://www.ncbi.nlm.nih.gov/pubmed/24586283
http://dx.doi.org/10.1371/journal.pone.0087593
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author Phelps, Nicholas B. D.
Mor, Sunil K.
Armien, Anibal G.
Batts, William
Goodwin, Andrew E.
Hopper, Lacey
McCann, Rebekah
Ng, Terry Fei Fan
Puzach, Corey
Waltzek, Thomas B.
Delwart, Eric
Winton, James
Goyal, Sagar M.
author_facet Phelps, Nicholas B. D.
Mor, Sunil K.
Armien, Anibal G.
Batts, William
Goodwin, Andrew E.
Hopper, Lacey
McCann, Rebekah
Ng, Terry Fei Fan
Puzach, Corey
Waltzek, Thomas B.
Delwart, Eric
Winton, James
Goyal, Sagar M.
author_sort Phelps, Nicholas B. D.
collection PubMed
description During both regulatory and routine surveillance sampling of baitfish from the states of Illinois, Minnesota, Montana, and Wisconsin, USA, isolates (n = 20) of a previously unknown picornavirus were obtained from kidney/spleen or entire viscera of fathead minnows (Pimephales promelas) and brassy minnows (Hybognathus hankinsoni). Following the appearance of a diffuse cytopathic effect, examination of cell culture supernatant by negative contrast electron microscopy revealed the presence of small, round virus particles (∼30–32 nm), with picornavirus-like morphology. Amplification and sequence analysis of viral RNA identified the agent as a novel member of the Picornaviridae family, tentatively named fathead minnow picornavirus (FHMPV). The full FHMPV genome consisted of 7834 nucleotides. Phylogenetic analysis based on 491 amino acid residues of the 3D gene showed 98.6% to 100% identity among the 20 isolates of FHMPV compared in this study while only 49.5% identity with its nearest neighbor, the bluegill picornavirus (BGPV) isolated from bluegill (Lepomis macrochirus). Based on complete polyprotein analysis, the FHMPV shared 58% (P1), 33% (P2) and 43% (P3) amino acid identities with BGPV and shared less than 40% amino acid identity with all other picornaviruses. Hence, we propose the creation of a new genus (Piscevirus) within the Picornaviridae family. The impact of FHMPV on the health of fish populations is unknown at present.
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spelling pubmed-39316142014-02-25 Isolation and Molecular Characterization of a Novel Picornavirus from Baitfish in the USA Phelps, Nicholas B. D. Mor, Sunil K. Armien, Anibal G. Batts, William Goodwin, Andrew E. Hopper, Lacey McCann, Rebekah Ng, Terry Fei Fan Puzach, Corey Waltzek, Thomas B. Delwart, Eric Winton, James Goyal, Sagar M. PLoS One Research Article During both regulatory and routine surveillance sampling of baitfish from the states of Illinois, Minnesota, Montana, and Wisconsin, USA, isolates (n = 20) of a previously unknown picornavirus were obtained from kidney/spleen or entire viscera of fathead minnows (Pimephales promelas) and brassy minnows (Hybognathus hankinsoni). Following the appearance of a diffuse cytopathic effect, examination of cell culture supernatant by negative contrast electron microscopy revealed the presence of small, round virus particles (∼30–32 nm), with picornavirus-like morphology. Amplification and sequence analysis of viral RNA identified the agent as a novel member of the Picornaviridae family, tentatively named fathead minnow picornavirus (FHMPV). The full FHMPV genome consisted of 7834 nucleotides. Phylogenetic analysis based on 491 amino acid residues of the 3D gene showed 98.6% to 100% identity among the 20 isolates of FHMPV compared in this study while only 49.5% identity with its nearest neighbor, the bluegill picornavirus (BGPV) isolated from bluegill (Lepomis macrochirus). Based on complete polyprotein analysis, the FHMPV shared 58% (P1), 33% (P2) and 43% (P3) amino acid identities with BGPV and shared less than 40% amino acid identity with all other picornaviruses. Hence, we propose the creation of a new genus (Piscevirus) within the Picornaviridae family. The impact of FHMPV on the health of fish populations is unknown at present. Public Library of Science 2014-02-21 /pmc/articles/PMC3931614/ /pubmed/24586283 http://dx.doi.org/10.1371/journal.pone.0087593 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.
spellingShingle Research Article
Phelps, Nicholas B. D.
Mor, Sunil K.
Armien, Anibal G.
Batts, William
Goodwin, Andrew E.
Hopper, Lacey
McCann, Rebekah
Ng, Terry Fei Fan
Puzach, Corey
Waltzek, Thomas B.
Delwart, Eric
Winton, James
Goyal, Sagar M.
Isolation and Molecular Characterization of a Novel Picornavirus from Baitfish in the USA
title Isolation and Molecular Characterization of a Novel Picornavirus from Baitfish in the USA
title_full Isolation and Molecular Characterization of a Novel Picornavirus from Baitfish in the USA
title_fullStr Isolation and Molecular Characterization of a Novel Picornavirus from Baitfish in the USA
title_full_unstemmed Isolation and Molecular Characterization of a Novel Picornavirus from Baitfish in the USA
title_short Isolation and Molecular Characterization of a Novel Picornavirus from Baitfish in the USA
title_sort isolation and molecular characterization of a novel picornavirus from baitfish in the usa
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3931614/
https://www.ncbi.nlm.nih.gov/pubmed/24586283
http://dx.doi.org/10.1371/journal.pone.0087593
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