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Isolation and Molecular Characterization of a Novel Picornavirus from Baitfish in the USA
During both regulatory and routine surveillance sampling of baitfish from the states of Illinois, Minnesota, Montana, and Wisconsin, USA, isolates (n = 20) of a previously unknown picornavirus were obtained from kidney/spleen or entire viscera of fathead minnows (Pimephales promelas) and brassy minn...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3931614/ https://www.ncbi.nlm.nih.gov/pubmed/24586283 http://dx.doi.org/10.1371/journal.pone.0087593 |
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author | Phelps, Nicholas B. D. Mor, Sunil K. Armien, Anibal G. Batts, William Goodwin, Andrew E. Hopper, Lacey McCann, Rebekah Ng, Terry Fei Fan Puzach, Corey Waltzek, Thomas B. Delwart, Eric Winton, James Goyal, Sagar M. |
author_facet | Phelps, Nicholas B. D. Mor, Sunil K. Armien, Anibal G. Batts, William Goodwin, Andrew E. Hopper, Lacey McCann, Rebekah Ng, Terry Fei Fan Puzach, Corey Waltzek, Thomas B. Delwart, Eric Winton, James Goyal, Sagar M. |
author_sort | Phelps, Nicholas B. D. |
collection | PubMed |
description | During both regulatory and routine surveillance sampling of baitfish from the states of Illinois, Minnesota, Montana, and Wisconsin, USA, isolates (n = 20) of a previously unknown picornavirus were obtained from kidney/spleen or entire viscera of fathead minnows (Pimephales promelas) and brassy minnows (Hybognathus hankinsoni). Following the appearance of a diffuse cytopathic effect, examination of cell culture supernatant by negative contrast electron microscopy revealed the presence of small, round virus particles (∼30–32 nm), with picornavirus-like morphology. Amplification and sequence analysis of viral RNA identified the agent as a novel member of the Picornaviridae family, tentatively named fathead minnow picornavirus (FHMPV). The full FHMPV genome consisted of 7834 nucleotides. Phylogenetic analysis based on 491 amino acid residues of the 3D gene showed 98.6% to 100% identity among the 20 isolates of FHMPV compared in this study while only 49.5% identity with its nearest neighbor, the bluegill picornavirus (BGPV) isolated from bluegill (Lepomis macrochirus). Based on complete polyprotein analysis, the FHMPV shared 58% (P1), 33% (P2) and 43% (P3) amino acid identities with BGPV and shared less than 40% amino acid identity with all other picornaviruses. Hence, we propose the creation of a new genus (Piscevirus) within the Picornaviridae family. The impact of FHMPV on the health of fish populations is unknown at present. |
format | Online Article Text |
id | pubmed-3931614 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-39316142014-02-25 Isolation and Molecular Characterization of a Novel Picornavirus from Baitfish in the USA Phelps, Nicholas B. D. Mor, Sunil K. Armien, Anibal G. Batts, William Goodwin, Andrew E. Hopper, Lacey McCann, Rebekah Ng, Terry Fei Fan Puzach, Corey Waltzek, Thomas B. Delwart, Eric Winton, James Goyal, Sagar M. PLoS One Research Article During both regulatory and routine surveillance sampling of baitfish from the states of Illinois, Minnesota, Montana, and Wisconsin, USA, isolates (n = 20) of a previously unknown picornavirus were obtained from kidney/spleen or entire viscera of fathead minnows (Pimephales promelas) and brassy minnows (Hybognathus hankinsoni). Following the appearance of a diffuse cytopathic effect, examination of cell culture supernatant by negative contrast electron microscopy revealed the presence of small, round virus particles (∼30–32 nm), with picornavirus-like morphology. Amplification and sequence analysis of viral RNA identified the agent as a novel member of the Picornaviridae family, tentatively named fathead minnow picornavirus (FHMPV). The full FHMPV genome consisted of 7834 nucleotides. Phylogenetic analysis based on 491 amino acid residues of the 3D gene showed 98.6% to 100% identity among the 20 isolates of FHMPV compared in this study while only 49.5% identity with its nearest neighbor, the bluegill picornavirus (BGPV) isolated from bluegill (Lepomis macrochirus). Based on complete polyprotein analysis, the FHMPV shared 58% (P1), 33% (P2) and 43% (P3) amino acid identities with BGPV and shared less than 40% amino acid identity with all other picornaviruses. Hence, we propose the creation of a new genus (Piscevirus) within the Picornaviridae family. The impact of FHMPV on the health of fish populations is unknown at present. Public Library of Science 2014-02-21 /pmc/articles/PMC3931614/ /pubmed/24586283 http://dx.doi.org/10.1371/journal.pone.0087593 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. |
spellingShingle | Research Article Phelps, Nicholas B. D. Mor, Sunil K. Armien, Anibal G. Batts, William Goodwin, Andrew E. Hopper, Lacey McCann, Rebekah Ng, Terry Fei Fan Puzach, Corey Waltzek, Thomas B. Delwart, Eric Winton, James Goyal, Sagar M. Isolation and Molecular Characterization of a Novel Picornavirus from Baitfish in the USA |
title | Isolation and Molecular Characterization of a Novel Picornavirus from Baitfish in the USA |
title_full | Isolation and Molecular Characterization of a Novel Picornavirus from Baitfish in the USA |
title_fullStr | Isolation and Molecular Characterization of a Novel Picornavirus from Baitfish in the USA |
title_full_unstemmed | Isolation and Molecular Characterization of a Novel Picornavirus from Baitfish in the USA |
title_short | Isolation and Molecular Characterization of a Novel Picornavirus from Baitfish in the USA |
title_sort | isolation and molecular characterization of a novel picornavirus from baitfish in the usa |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3931614/ https://www.ncbi.nlm.nih.gov/pubmed/24586283 http://dx.doi.org/10.1371/journal.pone.0087593 |
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