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Major Membrane Protein TDE2508 Regulates Adhesive Potency in Treponema denticola
The cultivation and genetic manipulation of Treponema denticola, a Gram-negative oral spirochaeta associated with periodontal diseases, is still challenging. In this study, we formulated a simple medium based on a commercially available one, and established a transformation method with high efficien...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3931704/ https://www.ncbi.nlm.nih.gov/pubmed/24586498 http://dx.doi.org/10.1371/journal.pone.0089051 |
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author | Abiko, Yuki Nagano, Keiji Yoshida, Yasuo Yoshimura, Fuminobu |
author_facet | Abiko, Yuki Nagano, Keiji Yoshida, Yasuo Yoshimura, Fuminobu |
author_sort | Abiko, Yuki |
collection | PubMed |
description | The cultivation and genetic manipulation of Treponema denticola, a Gram-negative oral spirochaeta associated with periodontal diseases, is still challenging. In this study, we formulated a simple medium based on a commercially available one, and established a transformation method with high efficiency. We then analyzed proteins in a membrane fraction in T. denticola and identified 16 major membrane-associated proteins, and characterized one of them, TDE2508, whose biological function was not yet known. Although this protein, which exhibited a complex conformation, was presumably localized in the outer membrane, we did not find conclusive evidence that it was exposed on the cell surface. Intriguingly, a TDE2508-deficient mutant exhibited significantly increased biofilm formation and adherent activity on human gingival epithelial cells. However, the protein deficiency did not alter autoaggregation, coaggregation with Porphyromonas gingivalis, hemagglutination, cell surface hydrophobicity, motility, or expression of Msp which was reported to be an adherent molecule in this bacteria. In conclusion, the major membrane protein TDE2508 regulates biofilm formation and the adhesive potency of T. denticola, although the underlying mechanism remains unclear. |
format | Online Article Text |
id | pubmed-3931704 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-39317042014-02-25 Major Membrane Protein TDE2508 Regulates Adhesive Potency in Treponema denticola Abiko, Yuki Nagano, Keiji Yoshida, Yasuo Yoshimura, Fuminobu PLoS One Research Article The cultivation and genetic manipulation of Treponema denticola, a Gram-negative oral spirochaeta associated with periodontal diseases, is still challenging. In this study, we formulated a simple medium based on a commercially available one, and established a transformation method with high efficiency. We then analyzed proteins in a membrane fraction in T. denticola and identified 16 major membrane-associated proteins, and characterized one of them, TDE2508, whose biological function was not yet known. Although this protein, which exhibited a complex conformation, was presumably localized in the outer membrane, we did not find conclusive evidence that it was exposed on the cell surface. Intriguingly, a TDE2508-deficient mutant exhibited significantly increased biofilm formation and adherent activity on human gingival epithelial cells. However, the protein deficiency did not alter autoaggregation, coaggregation with Porphyromonas gingivalis, hemagglutination, cell surface hydrophobicity, motility, or expression of Msp which was reported to be an adherent molecule in this bacteria. In conclusion, the major membrane protein TDE2508 regulates biofilm formation and the adhesive potency of T. denticola, although the underlying mechanism remains unclear. Public Library of Science 2014-02-21 /pmc/articles/PMC3931704/ /pubmed/24586498 http://dx.doi.org/10.1371/journal.pone.0089051 Text en © 2014 Abiko et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Abiko, Yuki Nagano, Keiji Yoshida, Yasuo Yoshimura, Fuminobu Major Membrane Protein TDE2508 Regulates Adhesive Potency in Treponema denticola |
title | Major Membrane Protein TDE2508 Regulates Adhesive Potency in Treponema denticola
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title_full | Major Membrane Protein TDE2508 Regulates Adhesive Potency in Treponema denticola
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title_fullStr | Major Membrane Protein TDE2508 Regulates Adhesive Potency in Treponema denticola
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title_full_unstemmed | Major Membrane Protein TDE2508 Regulates Adhesive Potency in Treponema denticola
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title_short | Major Membrane Protein TDE2508 Regulates Adhesive Potency in Treponema denticola
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title_sort | major membrane protein tde2508 regulates adhesive potency in treponema denticola |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3931704/ https://www.ncbi.nlm.nih.gov/pubmed/24586498 http://dx.doi.org/10.1371/journal.pone.0089051 |
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