Cargando…

A Multiplex Two-Color Real-Time PCR Method for Quality-Controlled Molecular Diagnostic Testing of FFPE Samples

BACKGROUND: Reverse transcription quantitative real-time PCR (RT-qPCR) tests support personalized cancer treatment through more clinically meaningful diagnosis. However, samples obtained through standard clinical pathology procedures are formalin-fixed, paraffin-embedded (FFPE) and yield small sampl...

Descripción completa

Detalles Bibliográficos
Autores principales:	Yeo, Jiyoun, Crawford, Erin L., Blomquist, Thomas M., Stanoszek, Lauren M., Dannemiller, Rachel E., Zyrek, Jill, De Las Casas, Luis E., Khuder, Sadik A., Willey, James C.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2014
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3931751/ https://www.ncbi.nlm.nih.gov/pubmed/24586747 http://dx.doi.org/10.1371/journal.pone.0089395

Ejemplares similares

Targeted RNA-Sequencing with Competitive Multiplex-PCR Amplicon Libraries
por: Blomquist, Thomas M., et al.
Publicado: (2013)

Correction: Targeted RNA-Sequencing with Competitive Multiplex-PCR Amplicon Libraries
por: Blomquist, Thomas M., et al.
Publicado: (2013)

A lung cancer risk classifier comprising genome maintenance genes measured in normal bronchial epithelial cells
por: Yeo, Jiyoun, et al.
Publicado: (2017)

Accurate Detection and Quantification of the Fish Viral Hemorrhagic Septicemia virus (VHSv) with a Two-Color Fluorometric Real-Time PCR Assay
por: Pierce, Lindsey R., et al.
Publicado: (2013)

Multiplex bisulfite PCR resequencing of clinical FFPE DNA
por: Korbie, Darren, et al.
Publicado: (2015)

Control for stochastic sampling variation and qualitative sequencing error in next generation sequencing
por: Blomquist, Thomas, et al.
Publicado: (2015)

A multiplex PCR predictor for aCGH success of FFPE samples
por: van Beers, E H, et al.
Publicado: (2006)

Technical advance in targeted NGS analysis enables identification of lung cancer risk-associated low frequency TP53, PIK3CA, and BRAF mutations in airway epithelial cells
por: Craig, Daniel J., et al.
Publicado: (2019)

Modified SureSelect(QXT) Target Enrichment Protocol for Illumina Multiplexed Sequencing of FFPE Samples
por: Rosa-Rosa, J. M., et al.
Publicado: (2018)

Choice of Illumination System & Fluorophore for Multiplex Immunofluorescence on FFPE Tissue Sections
por: Prost, Sandrine, et al.
Publicado: (2016)

ABCC5, ERCC2, XPA and XRCC1 transcript abundance levels correlate with cisplatin chemoresistance in non-small cell lung cancer cell lines
por: Weaver, David A, et al.
Publicado: (2005)

CEBPG transcription factor correlates with antioxidant and DNA repair genes in normal bronchial epithelial cells but not in individuals with bronchogenic carcinoma
por: Mullins, D'Anna N, et al.
Publicado: (2005)

Color-coded molecular beacons for multiplex PCR screening assays
por: Marras, Salvatore A. E., et al.
Publicado: (2019)

SimFFPE and FilterFFPE: improving structural variant calling in FFPE samples
por: Wei, Lanying, et al.
Publicado: (2021)

Using ddPCR to assess the DNA yield of FFPE samples
por: Lu, X.J. David, et al.
Publicado: (2018)

Deep oncopanel sequencing reveals within block position-dependent quality degradation in FFPE processed samples
por: Zhang, Yifan, et al.
Publicado: (2022)

Digital multiplexed analysis of circular RNAs in FFPE and fresh non‐small cell lung cancer specimens
por: Pedraz‐Valdunciel, Carlos, et al.
Publicado: (2022)

Targeted capture-based NGS is superior to multiplex PCR-based NGS for hereditary BRCA1 and BRCA2 gene analysis in FFPE tumor samples
por: Zakrzewski, Falk, et al.
Publicado: (2019)

Droplet digital PCR is an accurate method to assess methylation status on FFPE samples
por: Van Wesenbeeck, Liesbeth, et al.
Publicado: (2018)

Multiplexed characterization of cancer immune response in FFPE biopsies - initial performance of a new approach
por: Wang, Chichung, et al.
Publicado: (2013)

ChipCytometry for multiplexed detection of protein and mRNA markers on human FFPE tissue samples
por: Jarosch, Sebastian, et al.
Publicado: (2022)

RNAseq analysis of bronchial epithelial cells to identify COPD-associated genes and SNPs
por: Yeo, Jiyoun, et al.
Publicado: (2018)

Evaluation and validation of HPV real-time PCR assay for the detection of HPV DNA in oral cytobrush and FFPE samples
por: Harlé, Alexandre, et al.
Publicado: (2018)

Application of Real-Time PCR Assays for the Diagnosis of Histoplasmosis in Human FFPE Tissues Using Three Molecular Targets
por: López, Luisa F., et al.
Publicado: (2023)

Straightforward and sensitive RT-qPCR based gene expression analysis of FFPE samples
por: Zeka, Fjoralba, et al.
Publicado: (2016)

Cross-laboratory validation of the OncoScan® FFPE Assay, a multiplex tool for whole genome tumour profiling
por: Foster, Joseph M, et al.
Publicado: (2015)

Four-color multiplex real-time PCR assay prototype targeting azithromycin resistance mutations in Mycoplasma genitalium
por: Thellin, Olivier, et al.
Publicado: (2019)

Droplet digital PCR as an alternative to FISH for MYCN amplification detection in human neuroblastoma FFPE samples
por: Somasundaram, Dinesh Babu, et al.
Publicado: (2019)

SARS-CoV-2 RNA detection in Formalin-Fixed Paraffin-Embedded (FFPE) tissue by droplet digital PCR (ddPCR)
por: Majumdar, Ramanath, et al.
Publicado: (2022)

Detection of BRAF mutations in malignant melanoma and colorectal cancer by SensiScreen(®) FFPE BRAF qPCR assay
por: Sørensen, Anna Lahn, et al.
Publicado: (2023)

Combination of fluorescence color and melting temperature as a two-dimensional label for homogeneous multiplex PCR detection
por: Liao, Yiqun, et al.
Publicado: (2013)

The Use of Laser Microdissection in the Identification of Suitable Reference Genes for Normalization of Quantitative Real-Time PCR in Human FFPE Epithelial Ovarian Tissue Samples
por: Cai, Jing, et al.
Publicado: (2014)

High sensitivity sanger sequencing detection of BRAF mutations in metastatic melanoma FFPE tissue specimens
por: Cheng, Lauren Y., et al.
Publicado: (2021)

mRNA Capture Sequencing and RT-qPCR for the Detection of Pathognomonic, Novel, and Secondary Fusion Transcripts in FFPE Tissue: A Sarcoma Showcase
por: Decock, Anneleen, et al.
Publicado: (2022)

Determinants of RNA Quality from FFPE Samples
por: von Ahlfen, Silke, et al.
Publicado: (2007)

miRNA Profiles of Canine Intestinal Carcinomas, Lymphomas and Enteritis Analysed by Digital Droplet PCR from FFPE Material
por: Kehl, Alexandra, et al.
Publicado: (2023)

Prevalence of three Mycoplasma sp. by multiplex PCR in cattle with and without respiratory disease in central Mexico
por: Maya-Rodríguez, L. M., et al.
Publicado: (2022)

Fundamentals of multiplexing with digital PCR
por: Whale, Alexandra S., et al.
Publicado: (2016)

Multiplex Real-Time PCR Diagnostic of Relapsing Fevers in Africa
por: Elbir, Haitham, et al.
Publicado: (2013)

Multiplex real-time PCR for detection of respiratory tract infections
por: Brittain-Long, Robin, et al.
Publicado: (2008)

Cannot write session to /tmp/vufind_sessions/sess_lp73f9f24sm9jh11akr33bdtrm