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Involvement of Iron-Evoked Oxidative Stress in Smoking-Related Endothelial Dysfunction in Healthy Young Men
BACKGROUND: Oxidative stress and smoking contribute to endothelial dysfunction. Iron might also play a role in oxidative stress generation and endothelial dysfunction. However, the involvement of iron in smoking-induced endothelial dysfunction in healthy smokers remains unclear. Therefore, we examin...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3931785/ https://www.ncbi.nlm.nih.gov/pubmed/24586777 http://dx.doi.org/10.1371/journal.pone.0089433 |
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author | Fukami, Kei Yamagishi, Sho-ichi Iida, Shuji Matsuoka, Hidehiro Okuda, Seiya |
author_facet | Fukami, Kei Yamagishi, Sho-ichi Iida, Shuji Matsuoka, Hidehiro Okuda, Seiya |
author_sort | Fukami, Kei |
collection | PubMed |
description | BACKGROUND: Oxidative stress and smoking contribute to endothelial dysfunction. Iron might also play a role in oxidative stress generation and endothelial dysfunction. However, the involvement of iron in smoking-induced endothelial dysfunction in healthy smokers remains unclear. Therefore, we examined here whether (1) intravenous iron infusion impaired endothelial function evaluated by flow-mediated vasodilatation (FMD) in non-smokers, and (2) deferoxamine, a potent iron chelator, ameliorated endothelial dysfunction in healthy smokers. METHODS: Eight healthy young male non-smokers (23±4 years old) received intravenous injection of saccharated ferric oxide (0.7 mg/kg body weight), while 10 age-matched healthy male smokers received deferoxamine mesylate (8.3 mg/kg body weight). At baseline, 5 and 20 minutes after treatment with iron or deferoxamine, biochemical variables were measured, including serum iron and marondialdehyde (MDA), a marker of lipid oxidation, and endothelial function was simultaneously evaluated by FMD. RESULTS: Compared with non-smokers, FMD was significantly lower in smokers. Iron and MDA levels were significantly increased, whereas FMD was impaired by iron infusion in non-smokers. Conversely, deferoxamine treatment significantly decreased iron and MDA levels and restored the decreased FMD in smokers. Baseline serum iron and MDA levels in all 18 subjects (non-smokers and smokers) were correlated with each other. There was a significant inverse correlation between the changes in MDA values and FMD from baseline in 18 men. Endothelium-independent vasodilation by glyceryl trinitrate was unaltered by either treatment. CONCLUSIONS: Our present study suggests that iron-evoked oxidative stress might play a role in endothelial dysfunction in healthy smokers. |
format | Online Article Text |
id | pubmed-3931785 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-39317852014-02-25 Involvement of Iron-Evoked Oxidative Stress in Smoking-Related Endothelial Dysfunction in Healthy Young Men Fukami, Kei Yamagishi, Sho-ichi Iida, Shuji Matsuoka, Hidehiro Okuda, Seiya PLoS One Research Article BACKGROUND: Oxidative stress and smoking contribute to endothelial dysfunction. Iron might also play a role in oxidative stress generation and endothelial dysfunction. However, the involvement of iron in smoking-induced endothelial dysfunction in healthy smokers remains unclear. Therefore, we examined here whether (1) intravenous iron infusion impaired endothelial function evaluated by flow-mediated vasodilatation (FMD) in non-smokers, and (2) deferoxamine, a potent iron chelator, ameliorated endothelial dysfunction in healthy smokers. METHODS: Eight healthy young male non-smokers (23±4 years old) received intravenous injection of saccharated ferric oxide (0.7 mg/kg body weight), while 10 age-matched healthy male smokers received deferoxamine mesylate (8.3 mg/kg body weight). At baseline, 5 and 20 minutes after treatment with iron or deferoxamine, biochemical variables were measured, including serum iron and marondialdehyde (MDA), a marker of lipid oxidation, and endothelial function was simultaneously evaluated by FMD. RESULTS: Compared with non-smokers, FMD was significantly lower in smokers. Iron and MDA levels were significantly increased, whereas FMD was impaired by iron infusion in non-smokers. Conversely, deferoxamine treatment significantly decreased iron and MDA levels and restored the decreased FMD in smokers. Baseline serum iron and MDA levels in all 18 subjects (non-smokers and smokers) were correlated with each other. There was a significant inverse correlation between the changes in MDA values and FMD from baseline in 18 men. Endothelium-independent vasodilation by glyceryl trinitrate was unaltered by either treatment. CONCLUSIONS: Our present study suggests that iron-evoked oxidative stress might play a role in endothelial dysfunction in healthy smokers. Public Library of Science 2014-02-21 /pmc/articles/PMC3931785/ /pubmed/24586777 http://dx.doi.org/10.1371/journal.pone.0089433 Text en © 2014 Fukami et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Fukami, Kei Yamagishi, Sho-ichi Iida, Shuji Matsuoka, Hidehiro Okuda, Seiya Involvement of Iron-Evoked Oxidative Stress in Smoking-Related Endothelial Dysfunction in Healthy Young Men |
title | Involvement of Iron-Evoked Oxidative Stress in Smoking-Related Endothelial Dysfunction in Healthy Young Men |
title_full | Involvement of Iron-Evoked Oxidative Stress in Smoking-Related Endothelial Dysfunction in Healthy Young Men |
title_fullStr | Involvement of Iron-Evoked Oxidative Stress in Smoking-Related Endothelial Dysfunction in Healthy Young Men |
title_full_unstemmed | Involvement of Iron-Evoked Oxidative Stress in Smoking-Related Endothelial Dysfunction in Healthy Young Men |
title_short | Involvement of Iron-Evoked Oxidative Stress in Smoking-Related Endothelial Dysfunction in Healthy Young Men |
title_sort | involvement of iron-evoked oxidative stress in smoking-related endothelial dysfunction in healthy young men |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3931785/ https://www.ncbi.nlm.nih.gov/pubmed/24586777 http://dx.doi.org/10.1371/journal.pone.0089433 |
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