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Comparison of Reverse Transcription Quantitative Real-Time PCR, Flow Cytometry, and Immunohistochemistry for Detection of Monoclonality in Lymphomas

In healthy humans, 60–70% of the B lymphocytes produce kappa light chains, while the remaining cells produce lambda light chains. Malignant transformation and clonal expansion of B lymphocytes lead to an altered kappa : lambda expression ratio, which is an important diagnostic criteria of lymphomas....

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Autores principales: Ståhlberg, Anders, Åman, Pierre, Strömbom, Linda, Zoric, Neven, Diez, Alfredo, Nilsson, Olle, Kubista, Mikael, Ridell, Börje
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3932212/
https://www.ncbi.nlm.nih.gov/pubmed/24649374
http://dx.doi.org/10.1155/2014/796210
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author Ståhlberg, Anders
Åman, Pierre
Strömbom, Linda
Zoric, Neven
Diez, Alfredo
Nilsson, Olle
Kubista, Mikael
Ridell, Börje
author_facet Ståhlberg, Anders
Åman, Pierre
Strömbom, Linda
Zoric, Neven
Diez, Alfredo
Nilsson, Olle
Kubista, Mikael
Ridell, Börje
author_sort Ståhlberg, Anders
collection PubMed
description In healthy humans, 60–70% of the B lymphocytes produce kappa light chains, while the remaining cells produce lambda light chains. Malignant transformation and clonal expansion of B lymphocytes lead to an altered kappa : lambda expression ratio, which is an important diagnostic criteria of lymphomas. Here, we compared three methods for clonality determination of suspected B cell lymphomas. Tumor biopsies from 55 patients with B cell malignancies, 5 B-lymphoid tumor cell lines, and 20 biopsies from patients with lymphadenitis were analyzed by immunohistochemistry, flow cytometry, and reverse transcription quantitative real-time PCR. Clonality was determined by immunohistochemistry in 52/53 cases, flow cytometry in 30/39 cases, and reverse transcription quantitative real-time PCR in 33/55 cases. In conclusion, immunohistochemistry was superior to flow cytometry and reverse transcription quantitative real-time PCR for clonality identification. Flow cytometry and reverse transcription quantitative real-time PCR analysis has complementary values. In a considerable number of cases tumor cells produced both kappa and lambda light chain transcripts, but only one type of light chain peptide was produced.
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spelling pubmed-39322122014-03-19 Comparison of Reverse Transcription Quantitative Real-Time PCR, Flow Cytometry, and Immunohistochemistry for Detection of Monoclonality in Lymphomas Ståhlberg, Anders Åman, Pierre Strömbom, Linda Zoric, Neven Diez, Alfredo Nilsson, Olle Kubista, Mikael Ridell, Börje ISRN Oncol Research Article In healthy humans, 60–70% of the B lymphocytes produce kappa light chains, while the remaining cells produce lambda light chains. Malignant transformation and clonal expansion of B lymphocytes lead to an altered kappa : lambda expression ratio, which is an important diagnostic criteria of lymphomas. Here, we compared three methods for clonality determination of suspected B cell lymphomas. Tumor biopsies from 55 patients with B cell malignancies, 5 B-lymphoid tumor cell lines, and 20 biopsies from patients with lymphadenitis were analyzed by immunohistochemistry, flow cytometry, and reverse transcription quantitative real-time PCR. Clonality was determined by immunohistochemistry in 52/53 cases, flow cytometry in 30/39 cases, and reverse transcription quantitative real-time PCR in 33/55 cases. In conclusion, immunohistochemistry was superior to flow cytometry and reverse transcription quantitative real-time PCR for clonality identification. Flow cytometry and reverse transcription quantitative real-time PCR analysis has complementary values. In a considerable number of cases tumor cells produced both kappa and lambda light chain transcripts, but only one type of light chain peptide was produced. Hindawi Publishing Corporation 2014-02-04 /pmc/articles/PMC3932212/ /pubmed/24649374 http://dx.doi.org/10.1155/2014/796210 Text en Copyright © 2014 Anders Ståhlberg et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Ståhlberg, Anders
Åman, Pierre
Strömbom, Linda
Zoric, Neven
Diez, Alfredo
Nilsson, Olle
Kubista, Mikael
Ridell, Börje
Comparison of Reverse Transcription Quantitative Real-Time PCR, Flow Cytometry, and Immunohistochemistry for Detection of Monoclonality in Lymphomas
title Comparison of Reverse Transcription Quantitative Real-Time PCR, Flow Cytometry, and Immunohistochemistry for Detection of Monoclonality in Lymphomas
title_full Comparison of Reverse Transcription Quantitative Real-Time PCR, Flow Cytometry, and Immunohistochemistry for Detection of Monoclonality in Lymphomas
title_fullStr Comparison of Reverse Transcription Quantitative Real-Time PCR, Flow Cytometry, and Immunohistochemistry for Detection of Monoclonality in Lymphomas
title_full_unstemmed Comparison of Reverse Transcription Quantitative Real-Time PCR, Flow Cytometry, and Immunohistochemistry for Detection of Monoclonality in Lymphomas
title_short Comparison of Reverse Transcription Quantitative Real-Time PCR, Flow Cytometry, and Immunohistochemistry for Detection of Monoclonality in Lymphomas
title_sort comparison of reverse transcription quantitative real-time pcr, flow cytometry, and immunohistochemistry for detection of monoclonality in lymphomas
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3932212/
https://www.ncbi.nlm.nih.gov/pubmed/24649374
http://dx.doi.org/10.1155/2014/796210
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