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Mass Spectrometric Screening of Ovarian Cancer with Serum Glycans
Changes of glycosylation pattern in serum proteins have been linked to various diseases including cancer, suggesting possible development of novel biomarkers based on the glycomic analysis. In this study, N-linked glycans from human serum were quantitatively profiled by matrix-assisted laser desorpt...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3932261/ https://www.ncbi.nlm.nih.gov/pubmed/24648610 http://dx.doi.org/10.1155/2014/634289 |
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author | Kim, Jae-Han Park, Chang Won Um, Dalho Baek, Ki Hwang Jo, Yohahn An, Hyunjoo Kim, Yangsun Kim, Tae Jin |
author_facet | Kim, Jae-Han Park, Chang Won Um, Dalho Baek, Ki Hwang Jo, Yohahn An, Hyunjoo Kim, Yangsun Kim, Tae Jin |
author_sort | Kim, Jae-Han |
collection | PubMed |
description | Changes of glycosylation pattern in serum proteins have been linked to various diseases including cancer, suggesting possible development of novel biomarkers based on the glycomic analysis. In this study, N-linked glycans from human serum were quantitatively profiled by matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) and compared between healthy controls and ovarian cancer patients. A training set consisting of 40 healthy controls and 40 ovarian cancer cases demonstrated an inverse correlation between P value of ANOVA and area under the curve (AUC) of each candidate biomarker peak from MALDI-TOF MS, providing standards for the classification. A multibiomarker panel composed of 15 MALDI-TOF MS peaks resulted in AUC of 0.89, 80~90% sensitivity, and 70~83% specificity in the training set. The performance of the biomarker panel was validated in a separate blind test set composed of 23 healthy controls and 37 ovarian cancer patients, leading to 81~84% sensitivity and 83% specificity with cut-off values determined by the training set. Sensitivity of CA-125, the most widely used ovarian cancer marker, was 74% in the training set and 78% in the test set, respectively. These results indicate that MALDI-TOF MS-mediated serum N-glycan analysis could provide critical information for the screening of ovarian cancer. |
format | Online Article Text |
id | pubmed-3932261 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-39322612014-03-19 Mass Spectrometric Screening of Ovarian Cancer with Serum Glycans Kim, Jae-Han Park, Chang Won Um, Dalho Baek, Ki Hwang Jo, Yohahn An, Hyunjoo Kim, Yangsun Kim, Tae Jin Dis Markers Research Article Changes of glycosylation pattern in serum proteins have been linked to various diseases including cancer, suggesting possible development of novel biomarkers based on the glycomic analysis. In this study, N-linked glycans from human serum were quantitatively profiled by matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) and compared between healthy controls and ovarian cancer patients. A training set consisting of 40 healthy controls and 40 ovarian cancer cases demonstrated an inverse correlation between P value of ANOVA and area under the curve (AUC) of each candidate biomarker peak from MALDI-TOF MS, providing standards for the classification. A multibiomarker panel composed of 15 MALDI-TOF MS peaks resulted in AUC of 0.89, 80~90% sensitivity, and 70~83% specificity in the training set. The performance of the biomarker panel was validated in a separate blind test set composed of 23 healthy controls and 37 ovarian cancer patients, leading to 81~84% sensitivity and 83% specificity with cut-off values determined by the training set. Sensitivity of CA-125, the most widely used ovarian cancer marker, was 74% in the training set and 78% in the test set, respectively. These results indicate that MALDI-TOF MS-mediated serum N-glycan analysis could provide critical information for the screening of ovarian cancer. Hindawi Publishing Corporation 2014 2014-02-04 /pmc/articles/PMC3932261/ /pubmed/24648610 http://dx.doi.org/10.1155/2014/634289 Text en Copyright © 2014 Jae-Han Kim et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Kim, Jae-Han Park, Chang Won Um, Dalho Baek, Ki Hwang Jo, Yohahn An, Hyunjoo Kim, Yangsun Kim, Tae Jin Mass Spectrometric Screening of Ovarian Cancer with Serum Glycans |
title | Mass Spectrometric Screening of Ovarian Cancer with Serum Glycans |
title_full | Mass Spectrometric Screening of Ovarian Cancer with Serum Glycans |
title_fullStr | Mass Spectrometric Screening of Ovarian Cancer with Serum Glycans |
title_full_unstemmed | Mass Spectrometric Screening of Ovarian Cancer with Serum Glycans |
title_short | Mass Spectrometric Screening of Ovarian Cancer with Serum Glycans |
title_sort | mass spectrometric screening of ovarian cancer with serum glycans |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3932261/ https://www.ncbi.nlm.nih.gov/pubmed/24648610 http://dx.doi.org/10.1155/2014/634289 |
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