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Bacteriophages use an expanded genetic code on evolutionary paths to higher fitness

Bioengineering advances have made it possible to fundamentally alter the genetic codes of organisms. However, the evolutionary consequences of expanding an organism's genetic code with a non-canonical amino acid are poorly understood. Here we show that bacteriophages evolved on a host that inco...

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Detalles Bibliográficos
Autores principales: Hammerling, Michael J., Ellefson, Jared W., Boutz, Daniel R., Marcotte, Edward M., Ellington, Andrew D., Barrick, Jeffrey E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3932624/
https://www.ncbi.nlm.nih.gov/pubmed/24487692
http://dx.doi.org/10.1038/nchembio.1450
Descripción
Sumario:Bioengineering advances have made it possible to fundamentally alter the genetic codes of organisms. However, the evolutionary consequences of expanding an organism's genetic code with a non-canonical amino acid are poorly understood. Here we show that bacteriophages evolved on a host that incorporates 3-iodotyrosine at the amber stop codon acquired neutral and beneficial mutations to this new amino acid in their proteins, demonstrating that an expanded genetic code increases evolvability.