Bacteriophages use an expanded genetic code on evolutionary paths to higher fitness

Bioengineering advances have made it possible to fundamentally alter the genetic codes of organisms. However, the evolutionary consequences of expanding an organism's genetic code with a non-canonical amino acid are poorly understood. Here we show that bacteriophages evolved on a host that inco...

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Autores principales: Hammerling, Michael J., Ellefson, Jared W., Boutz, Daniel R., Marcotte, Edward M., Ellington, Andrew D., Barrick, Jeffrey E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3932624/
https://www.ncbi.nlm.nih.gov/pubmed/24487692
http://dx.doi.org/10.1038/nchembio.1450
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author Hammerling, Michael J.
Ellefson, Jared W.
Boutz, Daniel R.
Marcotte, Edward M.
Ellington, Andrew D.
Barrick, Jeffrey E.
author_facet Hammerling, Michael J.
Ellefson, Jared W.
Boutz, Daniel R.
Marcotte, Edward M.
Ellington, Andrew D.
Barrick, Jeffrey E.
author_sort Hammerling, Michael J.
collection PubMed
description Bioengineering advances have made it possible to fundamentally alter the genetic codes of organisms. However, the evolutionary consequences of expanding an organism's genetic code with a non-canonical amino acid are poorly understood. Here we show that bacteriophages evolved on a host that incorporates 3-iodotyrosine at the amber stop codon acquired neutral and beneficial mutations to this new amino acid in their proteins, demonstrating that an expanded genetic code increases evolvability.
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spelling pubmed-39326242014-09-01 Bacteriophages use an expanded genetic code on evolutionary paths to higher fitness Hammerling, Michael J. Ellefson, Jared W. Boutz, Daniel R. Marcotte, Edward M. Ellington, Andrew D. Barrick, Jeffrey E. Nat Chem Biol Article Bioengineering advances have made it possible to fundamentally alter the genetic codes of organisms. However, the evolutionary consequences of expanding an organism's genetic code with a non-canonical amino acid are poorly understood. Here we show that bacteriophages evolved on a host that incorporates 3-iodotyrosine at the amber stop codon acquired neutral and beneficial mutations to this new amino acid in their proteins, demonstrating that an expanded genetic code increases evolvability. 2014-02-02 2014-03 /pmc/articles/PMC3932624/ /pubmed/24487692 http://dx.doi.org/10.1038/nchembio.1450 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Hammerling, Michael J.
Ellefson, Jared W.
Boutz, Daniel R.
Marcotte, Edward M.
Ellington, Andrew D.
Barrick, Jeffrey E.
Bacteriophages use an expanded genetic code on evolutionary paths to higher fitness
title Bacteriophages use an expanded genetic code on evolutionary paths to higher fitness
title_full Bacteriophages use an expanded genetic code on evolutionary paths to higher fitness
title_fullStr Bacteriophages use an expanded genetic code on evolutionary paths to higher fitness
title_full_unstemmed Bacteriophages use an expanded genetic code on evolutionary paths to higher fitness
title_short Bacteriophages use an expanded genetic code on evolutionary paths to higher fitness
title_sort bacteriophages use an expanded genetic code on evolutionary paths to higher fitness
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3932624/
https://www.ncbi.nlm.nih.gov/pubmed/24487692
http://dx.doi.org/10.1038/nchembio.1450
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