Quantitative Field Testing Heterodera glycines from Metagenomic DNA Samples Isolated Directly from Soil under Agronomic Production

A quantitative PCR procedure targeting the Heterodera glycines ortholog of the Caenorhabditis elegans uncoordinated-78 gene was developed. The procedure estimated the quantity of H. glycines from metagenomic DNA samples isolated directly from field soil under agronomic production. The estimation of...

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Detalles Bibliográficos
Autores principales: Li, Yan, Lawrence, Gary W., Lu, Shien, Balbalian, Clarissa, Klink, Vincent P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3933691/
https://www.ncbi.nlm.nih.gov/pubmed/24587100
http://dx.doi.org/10.1371/journal.pone.0089887
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author Li, Yan
Lawrence, Gary W.
Lu, Shien
Balbalian, Clarissa
Klink, Vincent P.
author_facet Li, Yan
Lawrence, Gary W.
Lu, Shien
Balbalian, Clarissa
Klink, Vincent P.
author_sort Li, Yan
collection PubMed
description A quantitative PCR procedure targeting the Heterodera glycines ortholog of the Caenorhabditis elegans uncoordinated-78 gene was developed. The procedure estimated the quantity of H. glycines from metagenomic DNA samples isolated directly from field soil under agronomic production. The estimation of H. glycines quantity was determined in soil samples having other soil dwelling plant parasitic nematodes including Hoplolaimus, predatory nematodes including Mononchus, free-living nematodes and biomass. The methodology provides a framework for molecular diagnostics of nematodes from metagenomic DNA isolated directly from field soil.
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spelling pubmed-39336912014-02-25 Quantitative Field Testing Heterodera glycines from Metagenomic DNA Samples Isolated Directly from Soil under Agronomic Production Li, Yan Lawrence, Gary W. Lu, Shien Balbalian, Clarissa Klink, Vincent P. PLoS One Research Article A quantitative PCR procedure targeting the Heterodera glycines ortholog of the Caenorhabditis elegans uncoordinated-78 gene was developed. The procedure estimated the quantity of H. glycines from metagenomic DNA samples isolated directly from field soil under agronomic production. The estimation of H. glycines quantity was determined in soil samples having other soil dwelling plant parasitic nematodes including Hoplolaimus, predatory nematodes including Mononchus, free-living nematodes and biomass. The methodology provides a framework for molecular diagnostics of nematodes from metagenomic DNA isolated directly from field soil. Public Library of Science 2014-02-24 /pmc/articles/PMC3933691/ /pubmed/24587100 http://dx.doi.org/10.1371/journal.pone.0089887 Text en © 2014 Li et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Li, Yan
Lawrence, Gary W.
Lu, Shien
Balbalian, Clarissa
Klink, Vincent P.
Quantitative Field Testing Heterodera glycines from Metagenomic DNA Samples Isolated Directly from Soil under Agronomic Production
title Quantitative Field Testing Heterodera glycines from Metagenomic DNA Samples Isolated Directly from Soil under Agronomic Production
title_full Quantitative Field Testing Heterodera glycines from Metagenomic DNA Samples Isolated Directly from Soil under Agronomic Production
title_fullStr Quantitative Field Testing Heterodera glycines from Metagenomic DNA Samples Isolated Directly from Soil under Agronomic Production
title_full_unstemmed Quantitative Field Testing Heterodera glycines from Metagenomic DNA Samples Isolated Directly from Soil under Agronomic Production
title_short Quantitative Field Testing Heterodera glycines from Metagenomic DNA Samples Isolated Directly from Soil under Agronomic Production
title_sort quantitative field testing heterodera glycines from metagenomic dna samples isolated directly from soil under agronomic production
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3933691/
https://www.ncbi.nlm.nih.gov/pubmed/24587100
http://dx.doi.org/10.1371/journal.pone.0089887
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