Development of a DNA Sensor Based on Alkanethiol Self-Assembled Monolayer-Modified Electrodes

An electrochemical DNA biosensor based on recognition of double or single stranded DNA (ds-DNA/ss-DNA) immobilised on a self-assembled modified gold electrode is presented for denaturalisation and hybridisation detection. DNA is covalently bond on a self assembled 3-mercaptopropionic acid monolayer...

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Autores principales: Loaiza, Óscar A., Campuzano, Susana, López-Berlanga, María, Pedrero, María, Pingarrón, José M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2005
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3933879/
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author Loaiza, Óscar A.
Campuzano, Susana
López-Berlanga, María
Pedrero, María
Pingarrón, José M.
author_facet Loaiza, Óscar A.
Campuzano, Susana
López-Berlanga, María
Pedrero, María
Pingarrón, José M.
author_sort Loaiza, Óscar A.
collection PubMed
description An electrochemical DNA biosensor based on recognition of double or single stranded DNA (ds-DNA/ss-DNA) immobilised on a self-assembled modified gold electrode is presented for denaturalisation and hybridisation detection. DNA is covalently bond on a self assembled 3-mercaptopropionic acid monolayer by using water soluble N-3-(dimethylaminopropyl)-N prime;ethylcarbodiimide hydrochloride (EDC) and N-hydroxisulfosuccinimide (NHSS) as linkers. The interaction between the immobilised DNA and methylene blue (MB) is investigated using square wave voltammetry (SWV). The increase or diminution of peak currents of the MB upon the hybridisation or denaturalisation event at the modified electrode surface is studied.
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spelling pubmed-39338792014-02-25 Development of a DNA Sensor Based on Alkanethiol Self-Assembled Monolayer-Modified Electrodes Loaiza, Óscar A. Campuzano, Susana López-Berlanga, María Pedrero, María Pingarrón, José M. Sensors (Basel) Article An electrochemical DNA biosensor based on recognition of double or single stranded DNA (ds-DNA/ss-DNA) immobilised on a self-assembled modified gold electrode is presented for denaturalisation and hybridisation detection. DNA is covalently bond on a self assembled 3-mercaptopropionic acid monolayer by using water soluble N-3-(dimethylaminopropyl)-N prime;ethylcarbodiimide hydrochloride (EDC) and N-hydroxisulfosuccinimide (NHSS) as linkers. The interaction between the immobilised DNA and methylene blue (MB) is investigated using square wave voltammetry (SWV). The increase or diminution of peak currents of the MB upon the hybridisation or denaturalisation event at the modified electrode surface is studied. Molecular Diversity Preservation International (MDPI) 2005-11-14 /pmc/articles/PMC3933879/ Text en © 2005 by MDPI (http://www.mdpi.org). Reproduction is permitted for noncommercial purposes.
spellingShingle Article
Loaiza, Óscar A.
Campuzano, Susana
López-Berlanga, María
Pedrero, María
Pingarrón, José M.
Development of a DNA Sensor Based on Alkanethiol Self-Assembled Monolayer-Modified Electrodes
title Development of a DNA Sensor Based on Alkanethiol Self-Assembled Monolayer-Modified Electrodes
title_full Development of a DNA Sensor Based on Alkanethiol Self-Assembled Monolayer-Modified Electrodes
title_fullStr Development of a DNA Sensor Based on Alkanethiol Self-Assembled Monolayer-Modified Electrodes
title_full_unstemmed Development of a DNA Sensor Based on Alkanethiol Self-Assembled Monolayer-Modified Electrodes
title_short Development of a DNA Sensor Based on Alkanethiol Self-Assembled Monolayer-Modified Electrodes
title_sort development of a dna sensor based on alkanethiol self-assembled monolayer-modified electrodes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3933879/
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