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Hematoxylin Staining Reveals a Decrease in Nucleolar Diameter of Pig Oocytes Before Germinal Vesicle Breakdown
During oocyte growth, the morphology of the nucleolus changes into a compact and homogenous structure. The compact nucleoli in full-grown oocytes are not stained by aceto-orcein staining or immunofluorescence staining. In this study, we developed a hematoxylin staining method for pig oocytes in whol...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Society for Reproduction and Development
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3934123/ https://www.ncbi.nlm.nih.gov/pubmed/23856597 http://dx.doi.org/10.1262/jrd.2012-182 |
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author | Nobata, Tadatoshi Kyougoku, Hirohisa Miyano, Takashi |
author_facet | Nobata, Tadatoshi Kyougoku, Hirohisa Miyano, Takashi |
author_sort | Nobata, Tadatoshi |
collection | PubMed |
description | During oocyte growth, the morphology of the nucleolus changes into a compact and homogenous structure. The compact nucleoli in full-grown oocytes are not stained by aceto-orcein staining or immunofluorescence staining. In this study, we developed a hematoxylin staining method for pig oocytes in whole-mount preparations to visualize the nucleoli. Nucleoli of growing and full-grown oocytes were stained blue with hematoxylin. Using this staining method, the changes in the oocyte nucleolus during maturation were examined. The nucleolar diameter gradually decreased in maturing oocytes (10.7 ± 0.1 μm to 9.0 ± 0.7 μm, P<0.05) before germinal vesicle breakdown (GVBD). The results suggest that the nucleolar volume of oocytes decreases before GVBD. |
format | Online Article Text |
id | pubmed-3934123 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | The Society for Reproduction and Development |
record_format | MEDLINE/PubMed |
spelling | pubmed-39341232014-03-06 Hematoxylin Staining Reveals a Decrease in Nucleolar Diameter of Pig Oocytes Before Germinal Vesicle Breakdown Nobata, Tadatoshi Kyougoku, Hirohisa Miyano, Takashi J Reprod Dev Technology Report During oocyte growth, the morphology of the nucleolus changes into a compact and homogenous structure. The compact nucleoli in full-grown oocytes are not stained by aceto-orcein staining or immunofluorescence staining. In this study, we developed a hematoxylin staining method for pig oocytes in whole-mount preparations to visualize the nucleoli. Nucleoli of growing and full-grown oocytes were stained blue with hematoxylin. Using this staining method, the changes in the oocyte nucleolus during maturation were examined. The nucleolar diameter gradually decreased in maturing oocytes (10.7 ± 0.1 μm to 9.0 ± 0.7 μm, P<0.05) before germinal vesicle breakdown (GVBD). The results suggest that the nucleolar volume of oocytes decreases before GVBD. The Society for Reproduction and Development 2013-06-15 2013-10 /pmc/articles/PMC3934123/ /pubmed/23856597 http://dx.doi.org/10.1262/jrd.2012-182 Text en ©2013 Society for Reproduction and Development http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. |
spellingShingle | Technology Report Nobata, Tadatoshi Kyougoku, Hirohisa Miyano, Takashi Hematoxylin Staining Reveals a Decrease in Nucleolar Diameter of Pig Oocytes Before Germinal Vesicle Breakdown |
title | Hematoxylin Staining Reveals a Decrease in Nucleolar Diameter of Pig
Oocytes Before Germinal Vesicle Breakdown |
title_full | Hematoxylin Staining Reveals a Decrease in Nucleolar Diameter of Pig
Oocytes Before Germinal Vesicle Breakdown |
title_fullStr | Hematoxylin Staining Reveals a Decrease in Nucleolar Diameter of Pig
Oocytes Before Germinal Vesicle Breakdown |
title_full_unstemmed | Hematoxylin Staining Reveals a Decrease in Nucleolar Diameter of Pig
Oocytes Before Germinal Vesicle Breakdown |
title_short | Hematoxylin Staining Reveals a Decrease in Nucleolar Diameter of Pig
Oocytes Before Germinal Vesicle Breakdown |
title_sort | hematoxylin staining reveals a decrease in nucleolar diameter of pig
oocytes before germinal vesicle breakdown |
topic | Technology Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3934123/ https://www.ncbi.nlm.nih.gov/pubmed/23856597 http://dx.doi.org/10.1262/jrd.2012-182 |
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