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In Vitro Fertilization and Development of Porcine Oocytes Matured in Follicular Fluid

This study was conducted to assess the fertilization and development of porcine oocytes matured in a solo follicular fluid (pFF) using different in vitro culture systems and insemination periods. Cumulus-oocyte complexes (COCs), follicular cells (FCs), and pFF were collected from the follicles of ov...

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Autores principales: AGUNG, Budiyanto, OTOI, Takeshige, FUCHIMOTO, Dai-ichiro, SENBON, Shoichiro, ONISHI, Akira, NAGAI, Takashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Society for Reproduction and Development 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3934192/
https://www.ncbi.nlm.nih.gov/pubmed/23428620
http://dx.doi.org/10.1262/jrd.2011-050
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author AGUNG, Budiyanto
OTOI, Takeshige
FUCHIMOTO, Dai-ichiro
SENBON, Shoichiro
ONISHI, Akira
NAGAI, Takashi
author_facet AGUNG, Budiyanto
OTOI, Takeshige
FUCHIMOTO, Dai-ichiro
SENBON, Shoichiro
ONISHI, Akira
NAGAI, Takashi
author_sort AGUNG, Budiyanto
collection PubMed
description This study was conducted to assess the fertilization and development of porcine oocytes matured in a solo follicular fluid (pFF) using different in vitro culture systems and insemination periods. Cumulus-oocyte complexes (COCs), follicular cells (FCs), and pFF were collected from the follicles of ovaries. The pFF was used as a maturation medium (MpFF) after supplementation with follicle stimulating hormone (FSH) and antibiotics. The COCs were matured in a 15 ml test tube containing 3.5 ml of MpFF with FCs (5.2 × 10(6) cells/ml; rotating culture system) or 2 ml of MpFF without FCs in a 35-mm petri dish (static culture system) for 44 to 48 h. After maturation culture, oocytes were co-incubated with frozen-thawed spermatozoa for 5 h and then cultured for 7 days. The total mean rates of sperm penetration, normal fertilization, male pronucleus (MPN) formation, cleavage, and development to the blastocyst stage of oocytes after insemination were significantly higher (P<0.01) in the rotating culture system than in the static culture system. In conclusion, compared with the static culture system, the rotating culture system is adequate for the production of developmentally competent porcine oocytes when MpFF is used as a maturation medium.
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spelling pubmed-39341922014-03-06 In Vitro Fertilization and Development of Porcine Oocytes Matured in Follicular Fluid AGUNG, Budiyanto OTOI, Takeshige FUCHIMOTO, Dai-ichiro SENBON, Shoichiro ONISHI, Akira NAGAI, Takashi J Reprod Dev Original Article This study was conducted to assess the fertilization and development of porcine oocytes matured in a solo follicular fluid (pFF) using different in vitro culture systems and insemination periods. Cumulus-oocyte complexes (COCs), follicular cells (FCs), and pFF were collected from the follicles of ovaries. The pFF was used as a maturation medium (MpFF) after supplementation with follicle stimulating hormone (FSH) and antibiotics. The COCs were matured in a 15 ml test tube containing 3.5 ml of MpFF with FCs (5.2 × 10(6) cells/ml; rotating culture system) or 2 ml of MpFF without FCs in a 35-mm petri dish (static culture system) for 44 to 48 h. After maturation culture, oocytes were co-incubated with frozen-thawed spermatozoa for 5 h and then cultured for 7 days. The total mean rates of sperm penetration, normal fertilization, male pronucleus (MPN) formation, cleavage, and development to the blastocyst stage of oocytes after insemination were significantly higher (P<0.01) in the rotating culture system than in the static culture system. In conclusion, compared with the static culture system, the rotating culture system is adequate for the production of developmentally competent porcine oocytes when MpFF is used as a maturation medium. The Society for Reproduction and Development 2013-02-20 2013-04 /pmc/articles/PMC3934192/ /pubmed/23428620 http://dx.doi.org/10.1262/jrd.2011-050 Text en ©2013 Society for Reproduction and Development http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
spellingShingle Original Article
AGUNG, Budiyanto
OTOI, Takeshige
FUCHIMOTO, Dai-ichiro
SENBON, Shoichiro
ONISHI, Akira
NAGAI, Takashi
In Vitro Fertilization and Development of Porcine Oocytes Matured in Follicular Fluid
title In Vitro Fertilization and Development of Porcine Oocytes Matured in Follicular Fluid
title_full In Vitro Fertilization and Development of Porcine Oocytes Matured in Follicular Fluid
title_fullStr In Vitro Fertilization and Development of Porcine Oocytes Matured in Follicular Fluid
title_full_unstemmed In Vitro Fertilization and Development of Porcine Oocytes Matured in Follicular Fluid
title_short In Vitro Fertilization and Development of Porcine Oocytes Matured in Follicular Fluid
title_sort in vitro fertilization and development of porcine oocytes matured in follicular fluid
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3934192/
https://www.ncbi.nlm.nih.gov/pubmed/23428620
http://dx.doi.org/10.1262/jrd.2011-050
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