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Daily variation in the electrophysiological activity of mouse medial habenula neurones
AbstractIntrinsic daily or circadian rhythms arise through the outputs of the master circadian clock in the brain's suprachiasmatic nuclei (SCN) as well as circadian oscillators in other brain sites and peripheral tissues. SCN neurones contain an intracellular molecular clock that drives these...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Blackwell Publishing Ltd
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3934703/ https://www.ncbi.nlm.nih.gov/pubmed/24247982 http://dx.doi.org/10.1113/jphysiol.2013.263319 |
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author | Sakhi, Kanwal Belle, Mino D C Gossan, Nicole Delagrange, Philippe Piggins, Hugh D |
author_facet | Sakhi, Kanwal Belle, Mino D C Gossan, Nicole Delagrange, Philippe Piggins, Hugh D |
author_sort | Sakhi, Kanwal |
collection | PubMed |
description | AbstractIntrinsic daily or circadian rhythms arise through the outputs of the master circadian clock in the brain's suprachiasmatic nuclei (SCN) as well as circadian oscillators in other brain sites and peripheral tissues. SCN neurones contain an intracellular molecular clock that drives these neurones to exhibit pronounced day–night differences in their electrical properties. The epithalamic medial habenula (MHb) expresses clock genes, but little is known about the bioelectric properties of mouse MHb neurones and their potential circadian characteristics. Therefore, in this study we used a brain slice preparation containing the MHb to determine the basic electrical properties of mouse MHb neurones with whole-cell patch clamp electrophysiology, and investigated whether these vary across the day–night cycle. MHb neurones (n = 230) showed heterogeneity in electrophysiological state, ranging from highly depolarised cells (∼ −25 to −30 mV) that are silent with no membrane activity or display depolarised low-amplitude membrane oscillations, to neurones that were moderately hyperpolarised (∼40 mV) and spontaneously discharging action potentials. These electrical states were largely intrinsically regulated and were influenced by the activation of small-conductance calcium-activated potassium channels. When considered as one population, MHb neurones showed significant circadian variation in their spontaneous firing rate and resting membrane potential. However, in recordings of MHb neurones from mice lacking the core molecular circadian clock, these temporal differences in MHb activity were absent, indicating that circadian clock signals actively regulate the timing of MHb neuronal states. These observations add to the extracellularly recorded rhythms seen in other brain areas and establish that circadian mechanisms can influence the membrane properties of neurones in extra-SCN sites. Collectively, the results of this study indicate that the MHb may function as an intrinsic secondary circadian oscillator in the brain, which can shape daily information flow in key brain processes, such as reward and addiction. |
format | Online Article Text |
id | pubmed-3934703 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Blackwell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-39347032014-05-22 Daily variation in the electrophysiological activity of mouse medial habenula neurones Sakhi, Kanwal Belle, Mino D C Gossan, Nicole Delagrange, Philippe Piggins, Hugh D J Physiol Neuroscience: Cellular/Molecular AbstractIntrinsic daily or circadian rhythms arise through the outputs of the master circadian clock in the brain's suprachiasmatic nuclei (SCN) as well as circadian oscillators in other brain sites and peripheral tissues. SCN neurones contain an intracellular molecular clock that drives these neurones to exhibit pronounced day–night differences in their electrical properties. The epithalamic medial habenula (MHb) expresses clock genes, but little is known about the bioelectric properties of mouse MHb neurones and their potential circadian characteristics. Therefore, in this study we used a brain slice preparation containing the MHb to determine the basic electrical properties of mouse MHb neurones with whole-cell patch clamp electrophysiology, and investigated whether these vary across the day–night cycle. MHb neurones (n = 230) showed heterogeneity in electrophysiological state, ranging from highly depolarised cells (∼ −25 to −30 mV) that are silent with no membrane activity or display depolarised low-amplitude membrane oscillations, to neurones that were moderately hyperpolarised (∼40 mV) and spontaneously discharging action potentials. These electrical states were largely intrinsically regulated and were influenced by the activation of small-conductance calcium-activated potassium channels. When considered as one population, MHb neurones showed significant circadian variation in their spontaneous firing rate and resting membrane potential. However, in recordings of MHb neurones from mice lacking the core molecular circadian clock, these temporal differences in MHb activity were absent, indicating that circadian clock signals actively regulate the timing of MHb neuronal states. These observations add to the extracellularly recorded rhythms seen in other brain areas and establish that circadian mechanisms can influence the membrane properties of neurones in extra-SCN sites. Collectively, the results of this study indicate that the MHb may function as an intrinsic secondary circadian oscillator in the brain, which can shape daily information flow in key brain processes, such as reward and addiction. Blackwell Publishing Ltd 2014-02-15 2013-12-16 /pmc/articles/PMC3934703/ /pubmed/24247982 http://dx.doi.org/10.1113/jphysiol.2013.263319 Text en © 2013 The Authors. The Journal of Physiology © 2013 The Physiological Society http://creativecommons.org/licenses/by/3.0/ This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Neuroscience: Cellular/Molecular Sakhi, Kanwal Belle, Mino D C Gossan, Nicole Delagrange, Philippe Piggins, Hugh D Daily variation in the electrophysiological activity of mouse medial habenula neurones |
title | Daily variation in the electrophysiological activity of mouse medial habenula neurones |
title_full | Daily variation in the electrophysiological activity of mouse medial habenula neurones |
title_fullStr | Daily variation in the electrophysiological activity of mouse medial habenula neurones |
title_full_unstemmed | Daily variation in the electrophysiological activity of mouse medial habenula neurones |
title_short | Daily variation in the electrophysiological activity of mouse medial habenula neurones |
title_sort | daily variation in the electrophysiological activity of mouse medial habenula neurones |
topic | Neuroscience: Cellular/Molecular |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3934703/ https://www.ncbi.nlm.nih.gov/pubmed/24247982 http://dx.doi.org/10.1113/jphysiol.2013.263319 |
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