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Polypeptide Multilayer Self-Assembly Studied by Ellipsometry

A polypeptide nanofilm made by layer-by-layer (LbL) self-assembly was built on a surface that mimics nonwoven, a material commonly used in wound dressings. Poly-L-lysine (PLL) and poly-L-glutamic acid (PLGA) are the building blocks of the nanofilm, which is intended as an enzymatically degradable li...

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Detalles Bibliográficos
Autores principales: Craig, Marina, Holmberg, Krister, Le Ru, Eric, Etchegoin, Pablo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3934801/
https://www.ncbi.nlm.nih.gov/pubmed/24660065
http://dx.doi.org/10.1155/2014/424697
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author Craig, Marina
Holmberg, Krister
Le Ru, Eric
Etchegoin, Pablo
author_facet Craig, Marina
Holmberg, Krister
Le Ru, Eric
Etchegoin, Pablo
author_sort Craig, Marina
collection PubMed
description A polypeptide nanofilm made by layer-by-layer (LbL) self-assembly was built on a surface that mimics nonwoven, a material commonly used in wound dressings. Poly-L-lysine (PLL) and poly-L-glutamic acid (PLGA) are the building blocks of the nanofilm, which is intended as an enzymatically degradable lid for release of bactericides to chronic wounds. Chronic wounds often carry infection originating from bacteria such as Staphylococcus aureus and a release system triggered by the degree of infection is of interest. The dry nanofilm was studied with ellipsometry. The thickness of the nanofilm was 60% less in its dry state than in its wet state. The measurements showed that a primer was not necessary to build a stable nanofilm, which is practically important in our case because a nondegradable primer is highly unwanted in a wound care dressing. Added V8 (glutamyl endopeptidase) enzymes only showed adsorption on the nanofilm at room temperature, indicating that the PLL/PLGA “lid” may remain intact until the dressing has been filled with wound exudate at the elevated temperature typical of that of the wound.
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spelling pubmed-39348012014-03-23 Polypeptide Multilayer Self-Assembly Studied by Ellipsometry Craig, Marina Holmberg, Krister Le Ru, Eric Etchegoin, Pablo J Drug Deliv Research Article A polypeptide nanofilm made by layer-by-layer (LbL) self-assembly was built on a surface that mimics nonwoven, a material commonly used in wound dressings. Poly-L-lysine (PLL) and poly-L-glutamic acid (PLGA) are the building blocks of the nanofilm, which is intended as an enzymatically degradable lid for release of bactericides to chronic wounds. Chronic wounds often carry infection originating from bacteria such as Staphylococcus aureus and a release system triggered by the degree of infection is of interest. The dry nanofilm was studied with ellipsometry. The thickness of the nanofilm was 60% less in its dry state than in its wet state. The measurements showed that a primer was not necessary to build a stable nanofilm, which is practically important in our case because a nondegradable primer is highly unwanted in a wound care dressing. Added V8 (glutamyl endopeptidase) enzymes only showed adsorption on the nanofilm at room temperature, indicating that the PLL/PLGA “lid” may remain intact until the dressing has been filled with wound exudate at the elevated temperature typical of that of the wound. Hindawi Publishing Corporation 2014 2014-02-10 /pmc/articles/PMC3934801/ /pubmed/24660065 http://dx.doi.org/10.1155/2014/424697 Text en Copyright © 2014 Marina Craig et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Craig, Marina
Holmberg, Krister
Le Ru, Eric
Etchegoin, Pablo
Polypeptide Multilayer Self-Assembly Studied by Ellipsometry
title Polypeptide Multilayer Self-Assembly Studied by Ellipsometry
title_full Polypeptide Multilayer Self-Assembly Studied by Ellipsometry
title_fullStr Polypeptide Multilayer Self-Assembly Studied by Ellipsometry
title_full_unstemmed Polypeptide Multilayer Self-Assembly Studied by Ellipsometry
title_short Polypeptide Multilayer Self-Assembly Studied by Ellipsometry
title_sort polypeptide multilayer self-assembly studied by ellipsometry
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3934801/
https://www.ncbi.nlm.nih.gov/pubmed/24660065
http://dx.doi.org/10.1155/2014/424697
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