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Improvement of Functional Recovery of Donor Heart Following Cold Static Storage with Doxycycline Cardioplegia

Injury to the donor heart during cold preservation has a negative impact on graft survival before transplantation. This study aims to examine whether doxycycline, known as an MMP-2 inhibitor, has a positive effect on donor heart preservation via its antioxidant action when added to standard preserva...

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Autores principales: Ozcinar, Evren, Okatan, Esma N., Tuncay, Erkan, Eryilmaz, Sadik, Turan, Belma
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3936127/
https://www.ncbi.nlm.nih.gov/pubmed/24104944
http://dx.doi.org/10.1007/s12012-013-9231-1
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author Ozcinar, Evren
Okatan, Esma N.
Tuncay, Erkan
Eryilmaz, Sadik
Turan, Belma
author_facet Ozcinar, Evren
Okatan, Esma N.
Tuncay, Erkan
Eryilmaz, Sadik
Turan, Belma
author_sort Ozcinar, Evren
collection PubMed
description Injury to the donor heart during cold preservation has a negative impact on graft survival before transplantation. This study aims to examine whether doxycycline, known as an MMP-2 inhibitor, has a positive effect on donor heart preservation via its antioxidant action when added to standard preservation solution. Hearts were obtained from 3-month-old male Wistar rats and randomly divided into three groups: hearts stored for 1 h at 4 °C (1) with doxycycline preservation solution (DOX cardioplegia) with low Ca(2+); (2) with standard cardioplegia with low Ca(2+); and (3) unstored hearts. All hearts were perfused in working mode, arrested at 37 °C, removed from the perfusion system, reattached in Langendorff perfusion system, and converted to working mode for 1 h. At the end of the storage period, hearts preserved in DOX cardioplegia had significantly less weight gain than those preserved in the standard cardioplegia. DOX cardioplegia-induced preservation resulted in significantly higher heart rates and better recovery quality during reperfusion in aortic flow compared to the standard cardioplegia group. Recovery in the left ventricular function and Lambeth Convention Arrhythmia scores during 1 h reperfusion were also significantly better in the DOX cardioplegia group. Biochemical data showed that DOX cardioplegia prevented an increase in MMP-2 activity and blocked apoptosis through increased activity of the pro-survival kinase Akt in the donor heart homogenates. DOX cardioplegia also led to a balanced oxidant/antioxidant level in the heart homogenates. This is the first study to report that cardioplegia solution containing doxycycline provides better cardioprotection via the preservation of heart function, through its role in controlling cellular redox status during static cold storage.
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spelling pubmed-39361272014-03-05 Improvement of Functional Recovery of Donor Heart Following Cold Static Storage with Doxycycline Cardioplegia Ozcinar, Evren Okatan, Esma N. Tuncay, Erkan Eryilmaz, Sadik Turan, Belma Cardiovasc Toxicol Article Injury to the donor heart during cold preservation has a negative impact on graft survival before transplantation. This study aims to examine whether doxycycline, known as an MMP-2 inhibitor, has a positive effect on donor heart preservation via its antioxidant action when added to standard preservation solution. Hearts were obtained from 3-month-old male Wistar rats and randomly divided into three groups: hearts stored for 1 h at 4 °C (1) with doxycycline preservation solution (DOX cardioplegia) with low Ca(2+); (2) with standard cardioplegia with low Ca(2+); and (3) unstored hearts. All hearts were perfused in working mode, arrested at 37 °C, removed from the perfusion system, reattached in Langendorff perfusion system, and converted to working mode for 1 h. At the end of the storage period, hearts preserved in DOX cardioplegia had significantly less weight gain than those preserved in the standard cardioplegia. DOX cardioplegia-induced preservation resulted in significantly higher heart rates and better recovery quality during reperfusion in aortic flow compared to the standard cardioplegia group. Recovery in the left ventricular function and Lambeth Convention Arrhythmia scores during 1 h reperfusion were also significantly better in the DOX cardioplegia group. Biochemical data showed that DOX cardioplegia prevented an increase in MMP-2 activity and blocked apoptosis through increased activity of the pro-survival kinase Akt in the donor heart homogenates. DOX cardioplegia also led to a balanced oxidant/antioxidant level in the heart homogenates. This is the first study to report that cardioplegia solution containing doxycycline provides better cardioprotection via the preservation of heart function, through its role in controlling cellular redox status during static cold storage. Springer US 2013-10-09 2014 /pmc/articles/PMC3936127/ /pubmed/24104944 http://dx.doi.org/10.1007/s12012-013-9231-1 Text en © The Author(s) 2013 https://creativecommons.org/licenses/by/2.0/ Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Article
Ozcinar, Evren
Okatan, Esma N.
Tuncay, Erkan
Eryilmaz, Sadik
Turan, Belma
Improvement of Functional Recovery of Donor Heart Following Cold Static Storage with Doxycycline Cardioplegia
title Improvement of Functional Recovery of Donor Heart Following Cold Static Storage with Doxycycline Cardioplegia
title_full Improvement of Functional Recovery of Donor Heart Following Cold Static Storage with Doxycycline Cardioplegia
title_fullStr Improvement of Functional Recovery of Donor Heart Following Cold Static Storage with Doxycycline Cardioplegia
title_full_unstemmed Improvement of Functional Recovery of Donor Heart Following Cold Static Storage with Doxycycline Cardioplegia
title_short Improvement of Functional Recovery of Donor Heart Following Cold Static Storage with Doxycycline Cardioplegia
title_sort improvement of functional recovery of donor heart following cold static storage with doxycycline cardioplegia
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3936127/
https://www.ncbi.nlm.nih.gov/pubmed/24104944
http://dx.doi.org/10.1007/s12012-013-9231-1
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