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Application of Next Generation Sequencing Upon the Molecular Genetic Diagnosis of Deafness
The main objective of this review is to describe the new sequencing technologies called next generation sequencing (NGS) and its utility as a molecular genetic diagnosis tool in a medical field. Sanger method has dominated the genome sequencing industry for the past 30 years since its invention in 1...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Korean Audiological Society
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3936532/ https://www.ncbi.nlm.nih.gov/pubmed/24653861 http://dx.doi.org/10.7874/kja.2012.16.1.1 |
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author | Choi, Byung Yoon Kim, Bong Jik |
author_facet | Choi, Byung Yoon Kim, Bong Jik |
author_sort | Choi, Byung Yoon |
collection | PubMed |
description | The main objective of this review is to describe the new sequencing technologies called next generation sequencing (NGS) and its utility as a molecular genetic diagnosis tool in a medical field. Sanger method has dominated the genome sequencing industry for the past 30 years since its invention in 1975. It produced first human genome and still remains the gold standard for genome sequencing. However, it cannot meet the needs for enormous genetic data gathering and process because of its relatively long sequencing time and high cost per sample. NGS which parallelise the sequencing process, thereby increasing processing speed at a reduced cost per sample emerged to compensate for the weakness of the previous method. Currently NGS is used in some medical areas and its use is being widened. NGS also plays an important role in a study of genetically heterogenous hearing diseases. NGS is expected to mark a significant milestone in genomic research filed in a near future. |
format | Online Article Text |
id | pubmed-3936532 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | The Korean Audiological Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-39365322014-03-20 Application of Next Generation Sequencing Upon the Molecular Genetic Diagnosis of Deafness Choi, Byung Yoon Kim, Bong Jik Korean J Audiol Review The main objective of this review is to describe the new sequencing technologies called next generation sequencing (NGS) and its utility as a molecular genetic diagnosis tool in a medical field. Sanger method has dominated the genome sequencing industry for the past 30 years since its invention in 1975. It produced first human genome and still remains the gold standard for genome sequencing. However, it cannot meet the needs for enormous genetic data gathering and process because of its relatively long sequencing time and high cost per sample. NGS which parallelise the sequencing process, thereby increasing processing speed at a reduced cost per sample emerged to compensate for the weakness of the previous method. Currently NGS is used in some medical areas and its use is being widened. NGS also plays an important role in a study of genetically heterogenous hearing diseases. NGS is expected to mark a significant milestone in genomic research filed in a near future. The Korean Audiological Society 2012-04 2012-04-30 /pmc/articles/PMC3936532/ /pubmed/24653861 http://dx.doi.org/10.7874/kja.2012.16.1.1 Text en Copyright © 2012 The Korean Audiological Society http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Review Choi, Byung Yoon Kim, Bong Jik Application of Next Generation Sequencing Upon the Molecular Genetic Diagnosis of Deafness |
title | Application of Next Generation Sequencing Upon the Molecular Genetic Diagnosis of Deafness |
title_full | Application of Next Generation Sequencing Upon the Molecular Genetic Diagnosis of Deafness |
title_fullStr | Application of Next Generation Sequencing Upon the Molecular Genetic Diagnosis of Deafness |
title_full_unstemmed | Application of Next Generation Sequencing Upon the Molecular Genetic Diagnosis of Deafness |
title_short | Application of Next Generation Sequencing Upon the Molecular Genetic Diagnosis of Deafness |
title_sort | application of next generation sequencing upon the molecular genetic diagnosis of deafness |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3936532/ https://www.ncbi.nlm.nih.gov/pubmed/24653861 http://dx.doi.org/10.7874/kja.2012.16.1.1 |
work_keys_str_mv | AT choibyungyoon applicationofnextgenerationsequencinguponthemoleculargeneticdiagnosisofdeafness AT kimbongjik applicationofnextgenerationsequencinguponthemoleculargeneticdiagnosisofdeafness |