One-step zero-background IgG reformatting of phage-displayed antibody fragments enabling rapid and high-throughput lead identification

We describe a novel cloning method, referred to as insert-tagged (InTag) positive selection, for the rapid one-step reformatting of phage-displayed antibody fragments to full-length immunoglobulin Gs (IgGs). InTag positive selection enables recombinant clones of interest to be directly selected with...

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Detalles Bibliográficos
Autores principales: Chen, Chao-Guang, Fabri, Louis J., Wilson, Michael J., Panousis, Con
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2014
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3936716/
https://www.ncbi.nlm.nih.gov/pubmed/24253301
http://dx.doi.org/10.1093/nar/gkt1142

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3936716/
https://www.ncbi.nlm.nih.gov/pubmed/24253301
http://dx.doi.org/10.1093/nar/gkt1142

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