Cargando…

Phylogeny of Cas9 determines functional exchangeability of dual-RNA and Cas9 among orthologous type II CRISPR-Cas systems

The CRISPR-Cas-derived RNA-guided Cas9 endonuclease is the key element of an emerging promising technology for genome engineering in a broad range of cells and organisms. The DNA-targeting mechanism of the type II CRISPR-Cas system involves maturation of tracrRNA:crRNA duplex (dual-RNA), which direc...

Descripción completa

Detalles Bibliográficos
Autores principales:	Fonfara, Ines, Le Rhun, Anaïs, Chylinski, Krzysztof, Makarova, Kira S., Lécrivain, Anne-Laure, Bzdrenga, Janek, Koonin, Eugene V., Charpentier, Emmanuelle
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2014
Materias:	Nucleic Acid Enzymes
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3936727/ https://www.ncbi.nlm.nih.gov/pubmed/24270795 http://dx.doi.org/10.1093/nar/gkt1074

Ejemplares similares

PpCas9 from Pasteurella pneumotropica — a compact Type II-C Cas9 ortholog active in human cells
por: Fedorova, Iana, et al.
Publicado: (2020)

Key sequence features of CRISPR RNA for dual-guide CRISPR-Cas9 ribonucleoprotein complexes assembled with wild-type or HiFi Cas9
por: Okada, Keita, et al.
Publicado: (2022)

The tracrRNA and Cas9 families of type II CRISPR-Cas immunity systems
por: Chylinski, Krzysztof, et al.
Publicado: (2013)

Distinct patterns of Cas9 mismatch tolerance in vitro and in vivo
por: Fu, Becky X.H., et al.
Publicado: (2016)

DNA targeting by Clostridium cellulolyticum CRISPR–Cas9 Type II-C system
por: Fedorova, Iana, et al.
Publicado: (2020)

Cas6 specificity and CRISPR RNA loading in a complex CRISPR-Cas system
por: Sokolowski, Richard D., et al.
Publicado: (2014)

In-depth assessment of the PAM compatibility and editing activities of Cas9 variants
por: Zhang, Weiwei, et al.
Publicado: (2021)

Probing the stability of the SpCas9–DNA complex after cleavage
por: Aldag, Pierre, et al.
Publicado: (2021)

Discovery of potent and versatile CRISPR–Cas9 inhibitors engineered for chemically controllable genome editing
por: Song, Guoxu, et al.
Publicado: (2022)

Guide-specific loss of efficiency and off-target reduction with Cas9 variants
por: Zhang, Liang, et al.
Publicado: (2023)

Cas1–Cas2 physically and functionally interacts with DnaK to modulate CRISPR Adaptation
por: Killelea, Tom, et al.
Publicado: (2023)

Kinetics of the CRISPR-Cas9 effector complex assembly and the role of 3′-terminal segment of guide RNA
por: Mekler, Vladimir, et al.
Publicado: (2016)

Systematic in vitro specificity profiling reveals nicking defects in natural and engineered CRISPR–Cas9 variants
por: Murugan, Karthik, et al.
Publicado: (2021)

Bi-PE: bi-directional priming improves CRISPR/Cas9 prime editing in mammalian cells
por: Tao, Rui, et al.
Publicado: (2022)

Characterization of Cas12a nucleases reveals diverse PAM profiles between closely-related orthologs
por: Jacobsen, Thomas, et al.
Publicado: (2020)

Casposon integration shows strong target site preference and recapitulates protospacer integration by CRISPR-Cas systems
por: Béguin, Pierre, et al.
Publicado: (2016)

Targeted DNA transposition in vitro using a dCas9-transposase fusion protein
por: Bhatt, Shivam, et al.
Publicado: (2019)

Discovery and engineering of small SlugCas9 with broad targeting range and high specificity and activity
por: Hu, Ziying, et al.
Publicado: (2021)

DNA binding specificities of Escherichia coli Cas1–Cas2 integrase drive its recruitment at the CRISPR locus
por: Moch, Clara, et al.
Publicado: (2017)

Structure and specificity of the RNA-guided endonuclease Cas9 during DNA interrogation, target binding and cleavage
por: Josephs, Eric A., et al.
Publicado: (2015)

High-fidelity KKH variant of Staphylococcus aureus Cas9 nucleases with improved base mismatch discrimination
por: Yuen, Chaya T L, et al.
Publicado: (2022)

DNA rehybridization drives product release from Cas9 ribonucleoprotein to enable multiple-turnover cleavage
por: Pan, Juan, et al.
Publicado: (2023)

5′ modifications to CRISPR–Cas9 gRNA can change the dynamics and size of R-loops and inhibit DNA cleavage
por: Mullally, Grace, et al.
Publicado: (2020)

Efficient DNA interrogation of SpCas9 governed by its electrostatic interaction with DNA beyond the PAM and protospacer
por: Zhang, Qian, et al.
Publicado: (2021)

Active site plasticity enables metal-dependent tuning of Cas5d nuclease activity in CRISPR-Cas type I-C system
por: Punetha, Ankita, et al.
Publicado: (2014)

Mechanics of CRISPR-Cas12a and engineered variants on λ-DNA
por: Paul, Bijoya, et al.
Publicado: (2021)

Classification and evolution of type II CRISPR-Cas systems
por: Chylinski, Krzysztof, et al.
Publicado: (2014)

Asymmetric positioning of Cas1–2 complex and Integration Host Factor induced DNA bending guide the unidirectional homing of protospacer in CRISPR-Cas type I-E system
por: Yoganand, K.N.R., et al.
Publicado: (2017)

Modulating the Cascade architecture of a minimal Type I-F CRISPR-Cas system
por: Gleditzsch, Daniel, et al.
Publicado: (2016)

CRISPR-Cas12a targeting of ssDNA plays no detectable role in immunity
por: Marino, Nicole D, et al.
Publicado: (2022)

Enhanced Cas12a editing in mammalian cells and zebrafish
por: Liu, Pengpeng, et al.
Publicado: (2019)

In vitro assembly and activity of an archaeal CRISPR-Cas type I-A Cascade interference complex
por: Plagens, André, et al.
Publicado: (2014)

Interference activity of a minimal Type I CRISPR–Cas system from Shewanella putrefaciens
por: Dwarakanath, Srivatsa, et al.
Publicado: (2015)

Different modes of spacer acquisition by the Staphylococcus epidermidis type III-A CRISPR-Cas system
por: Aviram, Naama, et al.
Publicado: (2022)

Molecular basis of cyclic tetra-oligoadenylate processing by small standalone CRISPR-Cas ring nucleases
por: Molina, Rafael, et al.
Publicado: (2022)

A type III-B CRISPR-Cas effector complex mediating massive target DNA destruction
por: Han, Wenyuan, et al.
Publicado: (2017)

Enhancement of target specificity of CRISPR–Cas12a by using a chimeric DNA–RNA guide
por: Kim, Hanseop, et al.
Publicado: (2020)

A DNA unwinding equilibrium serves as a checkpoint for CRISPR-Cas12a target discrimination
por: Singh, Jaideep, et al.
Publicado: (2023)

PAM recognition by miniature CRISPR–Cas12f nucleases triggers programmable double-stranded DNA target cleavage
por: Karvelis, Tautvydas, et al.
Publicado: (2020)

Bacteriophage DNA glucosylation impairs target DNA binding by type I and II but not by type V CRISPR–Cas effector complexes
por: Vlot, Marnix, et al.
Publicado: (2018)

Cannot write session to /tmp/vufind_sessions/sess_n682a8jpn2p8paqeuo9bv5lj6o