Method for site-specific detection of m6A nucleoside presence in RNA based on high-resolution melting (HRM) analysis

Chemical landscape of natural RNA species is decorated with the large number of modified nucleosides. Some of those could easily be detected by reverse transcription, while others permit only high-performance liquid chromatography or mass-spectrometry detection. Presence of m(6)A nucleoside at a par...

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Autores principales: Golovina, Anna Y., Dzama, Margarita M., Petriukov, Kirill S., Zatsepin, Timofei S., Sergiev, Petr V., Bogdanov, Alexey A., Dontsova, Olga A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2014
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3936739/
https://www.ncbi.nlm.nih.gov/pubmed/24265225
http://dx.doi.org/10.1093/nar/gkt1160
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author Golovina, Anna Y.
Dzama, Margarita M.
Petriukov, Kirill S.
Zatsepin, Timofei S.
Sergiev, Petr V.
Bogdanov, Alexey A.
Dontsova, Olga A.
author_facet Golovina, Anna Y.
Dzama, Margarita M.
Petriukov, Kirill S.
Zatsepin, Timofei S.
Sergiev, Petr V.
Bogdanov, Alexey A.
Dontsova, Olga A.
author_sort Golovina, Anna Y.
collection PubMed
description Chemical landscape of natural RNA species is decorated with the large number of modified nucleosides. Some of those could easily be detected by reverse transcription, while others permit only high-performance liquid chromatography or mass-spectrometry detection. Presence of m(6)A nucleoside at a particular position of long RNA molecule is challenging to observe. Here we report an easy and high-throughput method for detection of m(6)A nucleosides in RNA based on high-resolution melting analysis. The method relies on the previous knowledge of the modified nucleoside position at a particular place of RNA and allows rapid screening for conditions or genes necessary for formation of that modification.
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spelling pubmed-39367392014-03-04 Method for site-specific detection of m6A nucleoside presence in RNA based on high-resolution melting (HRM) analysis Golovina, Anna Y. Dzama, Margarita M. Petriukov, Kirill S. Zatsepin, Timofei S. Sergiev, Petr V. Bogdanov, Alexey A. Dontsova, Olga A. Nucleic Acids Res Methods Online Chemical landscape of natural RNA species is decorated with the large number of modified nucleosides. Some of those could easily be detected by reverse transcription, while others permit only high-performance liquid chromatography or mass-spectrometry detection. Presence of m(6)A nucleoside at a particular position of long RNA molecule is challenging to observe. Here we report an easy and high-throughput method for detection of m(6)A nucleosides in RNA based on high-resolution melting analysis. The method relies on the previous knowledge of the modified nucleoside position at a particular place of RNA and allows rapid screening for conditions or genes necessary for formation of that modification. Oxford University Press 2014-02 2013-11-20 /pmc/articles/PMC3936739/ /pubmed/24265225 http://dx.doi.org/10.1093/nar/gkt1160 Text en © The Author(s) 2013. Published by Oxford University Press. http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Methods Online
Golovina, Anna Y.
Dzama, Margarita M.
Petriukov, Kirill S.
Zatsepin, Timofei S.
Sergiev, Petr V.
Bogdanov, Alexey A.
Dontsova, Olga A.
Method for site-specific detection of m6A nucleoside presence in RNA based on high-resolution melting (HRM) analysis
title Method for site-specific detection of m6A nucleoside presence in RNA based on high-resolution melting (HRM) analysis
title_full Method for site-specific detection of m6A nucleoside presence in RNA based on high-resolution melting (HRM) analysis
title_fullStr Method for site-specific detection of m6A nucleoside presence in RNA based on high-resolution melting (HRM) analysis
title_full_unstemmed Method for site-specific detection of m6A nucleoside presence in RNA based on high-resolution melting (HRM) analysis
title_short Method for site-specific detection of m6A nucleoside presence in RNA based on high-resolution melting (HRM) analysis
title_sort method for site-specific detection of m6a nucleoside presence in rna based on high-resolution melting (hrm) analysis
topic Methods Online
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3936739/
https://www.ncbi.nlm.nih.gov/pubmed/24265225
http://dx.doi.org/10.1093/nar/gkt1160
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