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Method for site-specific detection of m6A nucleoside presence in RNA based on high-resolution melting (HRM) analysis

Chemical landscape of natural RNA species is decorated with the large number of modified nucleosides. Some of those could easily be detected by reverse transcription, while others permit only high-performance liquid chromatography or mass-spectrometry detection. Presence of m(6)A nucleoside at a par...

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Detalles Bibliográficos
Autores principales:	Golovina, Anna Y., Dzama, Margarita M., Petriukov, Kirill S., Zatsepin, Timofei S., Sergiev, Petr V., Bogdanov, Alexey A., Dontsova, Olga A.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2014
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3936739/ https://www.ncbi.nlm.nih.gov/pubmed/24265225 http://dx.doi.org/10.1093/nar/gkt1160

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